ISOLATION AND CHARACTERIZATION OF MANNANOLYTIC THERMOPHILIC BACTERIA FROM PALM OIL SHELL AND THEIR MANNANASE ENZYME PRODUCTION PROPERTIES

Authors

  • ANTONIUS SUWANTO 2 Department of Biology, Faculty of Science and Mathematics, Bogor Agricultural University,
  • SUMARDI Department of Biology, Faculty of Science and Mathematics, University of Lampung, Jl. S. Brojonegoro No. 1, Bandar Lampung 35145, Indonesia
  • MAGGY T HENAWIDJAJA Department of Food Science and Human Nu trition, Bogor Agricultural University, Darmaga, 16680, Bogor, Indonesia
  • T RESNAWATI P URWADARIA Indonesian Research Institute for Animal Production, P.O. Box 221, Bogor 16002, Indonesia

DOI:

https://doi.org/10.11598/btb.2005.0.25.211

Abstract

A mannanolytic thermophilic bacterium (L-07) was isolated from palm oil shell after 2 days ofenrichment in liquid medium supplemented with 1% palm kernel meal as mannan source. Sequenceanalysis of 16S-rRNA indicated that L-07 was similar (98%) to  Geobacillus stearothermophilus, aspecies of thermophilic aerobic bacteria. We found that  G. stearothermophilus L-07 producedextracellular β-1,4-mannanases, but no β-manosidase and α-galactosidase activities. The growth of L-07reached its maximum (3.0 x 106 cell/ml) at 12-20 hours, while the highest  β-mannanase activity (0.52U/ml) was observed in culture medium after 36 hours of cultivation at 60oC. The medium containinglocust bean gum was the best for producing extracellular β-1,4-mannanases compared with kolang kaling,konjak, and palm kernel meal. SDS-PAGE and zymogram analysis demonstrated that crude mannanasecomplex of L-07 from locust bean gum containing medium comprised three active bands with molecularweight of 85, 73 and 50 kDa.   Keywords :  Extracellular enzyme/mannanase/Geobacillus stearothermophilus

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