ISOLATION AND CHARACTERIZATION OF MANNANOLYTIC THERMOPHILIC BACTERIA FROM PALM OIL SHELL AND THEIR MANNANASE ENZYME PRODUCTION PROPERTIES
A mannanolytic thermophilic bacterium (L-07) was isolated from palm oil shell after 2 days of
enrichment in liquid medium supplemented with 1% palm kernel meal as mannan source. Sequence
analysis of 16S-rRNA indicated that L-07 was similar (98%) toÂ Geobacillus stearothermophilus, a
species of thermophilic aerobic bacteria. We found thatÂ G. stearothermophilus L-07 produced
extracellular Î²-1,4-mannanases, but no Î²-manosidase and Î±-galactosidase activities. The growth of L-07
reached its maximum (3.0 x 106
Â cell/ml) at 12-20 hours, while the highestÂ Î²-mannanase activity (0.52
U/ml) was observed in culture medium after 36 hours of cultivation at 60o
C. The medium containing
locust bean gum was the best for producing extracellular Î²-1,4-mannanases compared with kolang kaling,
konjak, and palm kernel meal. SDS-PAGE and zymogram analysis demonstrated that crude mannanase
complex of L-07 from locust bean gum containing medium comprised three active bands with molecular
weight of 85, 73 and 50 kDa.Â
Keywords :Â Extracellular enzyme/mannanase/Geobacillus stearothermophilus
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How to Cite
SUWANTO, A., SUMARDI, HENAWIDJAJA, M. T., & URWADARIA, T. R. P. (2011). ISOLATION AND CHARACTERIZATION OF MANNANOLYTIC THERMOPHILIC BACTERIA FROM PALM OIL SHELL AND THEIR MANNANASE ENZYME PRODUCTION PROPERTIES. BIOTROPIA - The Southeast Asian Journal of Tropical Biology, (25). https://doi.org/10.11598/btb.2005.0.25.211
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