CHARACTERIZATION OF THREE BENZOATE DEGRADING ANOXYGENIC PHOTOSYNTHETIC BACTERIA ISOLATED FROM THE ENVIRONMENT
AbstractThreeÂ anoxygenic photosynthetic bacteria, DS-1, DS-4 and Cas-13, have been examinatedÂ forÂ themorphological and physiologicalÂ properties. All strains were rod-shape cells withÂ a swollen terminalÂ endGramÂ negative, motile, non-halophilic, non-alkalophilicÂ and non-acidophilic,Â and capable of utilizinbenzoate aerobically and photo-anaerobically. Sequence analysis of part of 16S rRNA genes showed that DS1 and Cas-13 were closely related to Rhodopseudomonas palustris Strain 7 with a similarity of 97%, whereaDS-4 may not be closely related to the former two strains with a similarity of 78% based on the constructephylogenicÂ tree. Spectral analysis indicated that the threeÂ bacteriaÂ hadÂ bacteriochlorophylÂ aÂ and normaspirilloxanthin series. Growth in medium enriched with vitamin and supplemented with benzoate as their sole C-sources wabetter than in medium without vitamin. Benzoate degradation in medium with vitamin was accelerated. ThabilityÂ to grow on benzoate without added vitamins indicatedÂ thatÂ the bacteria were able to synthesizeÂ theown vitamins. Key words: anoxygenic photosynthetic bacteria/ benzoate degradation/ 16S rRNA gene.
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