GENETIC DIVERSITY OF AMPICILLIN-RESISTANT Vibrio ISOLATED FROM VARIOUS STAGES OF TIGER SHRIMP LARVAE DEVELOPMENT
AbstractThis research was carriedÂ out toÂ studyÂ geneticÂ diversity of ampicillin-resistant VibrioÂ from
variousÂ stages of tigerÂ shrimp larvaeÂ (Penaeus Monodon) development from,Tambak Inti Rakyat
hatchery, near Labuan, West Java, Indonesia. A total of 25 ampicillin-resistant Vibrio isolates were
isolated using thiosulphateÂ citrate bile-saltÂ sucroseÂ agar (TCBS-Agar) and seawaterÂ complete
agar (SWC-Agar). Physiological and biochemical characterization showed that the isolates could be
grouped into only two species, i.e. V. harveyi from the egg stage; and V. metschnikovii from larvae
and post-larval stage (i.e nauplius, zoea, mysis, PLi, PL5, PL,0, and PL,5). These isolates were also
present in their respective rearing waterÂ ofÂ each stageÂ andÂ some natural feed.Â Schizotyping
analysis employing restriction endonuclease Noll (5'-GC4GGCCGC) indicated that the isolates
could be grouped intoÂ at leastÂ 13 differentÂ genotypes.Â Therefore,Â schizotypingÂ wasÂ more
discriminative than physiological characterization. This study showed that particular groups of Vibrio
colonized all stages of shrimp larvae and demonstrated closed phylogenetic relationship. These groups
of Vibrio might beÂ the dominant microbiota which could suppress the development of other Vibrio
including the pathogenic Vibrio.
Key words : Shrimp/ampicillin-resistant K/fcno/schizotyping
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How to Cite
WIDANARNI, W., & SUWANTO, A. (2011). GENETIC DIVERSITY OF AMPICILLIN-RESISTANT Vibrio ISOLATED FROM VARIOUS STAGES OF TIGER SHRIMP LARVAE DEVELOPMENT. BIOTROPIA, (15). https://doi.org/10.11598/btb.2000.0.15.160
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