A NOVEL INTEGRON IN THE GENOME OF ESCHERICHIA COLI ISOLATED FROM INDONESIAN MONITOR LIZARD (VARANUS SPP).
AbstractThe genotype of antibiotic resistanceÂ in natural isolates of Escherichia coli was determinedÂ through integron detection andÂ characterization of the associated antibioticÂ resistance. E. coli SG2 isolated from Varanus salvatorÂ of Java demonstratedÂ resistanceÂ to spectinomycin (50ng/ml)Â and streptomycin (SOng/ml). Integron detection indicatedÂ that eight isolates out of nine E. coliÂ isolates possessed a conserved segment of the integron. Amplification ofÂ the inserted cassette of the integron in this SG2 isolate yielded a 1-kb DNA fragment. Sequence analyses indicated that this fragment was homologous with aad gene, which confirmedÂ the resistanceÂ to spectinomycin/streptomycin. This is the first report on the presence of integron in the E. coli isolated from the environment. Key words: Integron / antibiotic resistance / Escherichia coli
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