HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENS
AbstractA transcriptional fusion for an Aeromonas caviae chitinase gene was constructed under the control of a constitutive
promoter of the kanaraycin resistance gene (PKmR
). The construct was inserted into a medium copy number broad host
range plasmid vector to yield recombinant plasmid pAM340, which harbored transcriptional fusion PKmR
- chi. Another
transcriptional fusion, Ptac-chi, in a recombinant plasmid pAM630, was conducted as comparison. Triparental mating of E.
coliÂ carrying the recombinant plasmids with Pseudomotws fluorescensÂ 5100, a phyllosphere bacterium, was performed.
Pseudomonas fluorescensÂ 5100 exconjugants were examined for constitutive expression of chitinase employing a
spectrophotometric assay; they showed stronger chitin degradation activity than Escherichia coli transformants. Using a fungal
antagonism plate assay, this chitinolytic P. fluorescens, however, could not inhibit selected phytopathogenic fungi.
Keywords:Â Â Â AeromonasÂ Â caviae/Â chitinaseÂ Â gene/transcriptionalÂ Â fusion/PKm'VÂ Â Vtac-chilPseudomonas fluorescens
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How to Cite
SUWANTO, A., MALIK, A., TJAHJONO, B., & HARLING, R. (2011). HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENS. BIOTROPIA - The Southeast Asian Journal of Tropical Biology, (20). https://doi.org/10.11598/btb.2003.0.20.182
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