SOLATION AND CHARACTERIZATION OF A NOVEL BENZOATE- UTILIZING Serratia marcescens
No. 21 (2003)
Research Paper
November 17, 2011
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A new benzoate-utilizing strain, Serratia marcescens DS-8, isolated from the environment was characterized. The
strain was enterobacilli, Gram negative, mesophilic, non halophilic, and aerobic bacterium that showed motile ovale-
rod shaped cells. The isolate produced extracellular chitinase, protease, and prodigiosin (a red pigment produced by
several Serratia strains yielding bright red or pink colonies). A physiological assay using Microbact* test showed that the
strain was closely related to Klebsiella ozaenae (49.85%) and Serratia liquefaciens (24.42%), respectively. However,
16S rRNA sequence analysis indicated that the strain was closely related to S. marcescens DSM 30121 with
similarity level of 98%. DS-8 strain was able to synthesize its own vitamins. Optimum growth in benzoate was
obtained at pH between 7-8.5 and NaCl concentration of 1-1.5% (w/v). The isolate could grow in benzoate-containing
medium up to 10 mM. Other carbon sources that could support the growth of DS-8 were casamino acid, glutamate, glucose,
acetate, potato starch, and ethanol.
Keywords: Serratia marcescens/aromatic degradation/168 rRNA sequence
SURYANTO, D., & SUWANTO, A. (2011). SOLATION AND CHARACTERIZATION OF A NOVEL BENZOATE- UTILIZING Serratia marcescens. BIOTROPIA, (21). https://doi.org/10.11598/btb.2003.0.21.184
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