OPTIMIZATION BACTERIAL DENSITY AND FERTILIZER DOSAGE FOR BIOREMEDIATION OF OIL CONTAMINATED SANDY BEACH: A CASE OF CILACAP, INDONESIA

Article Sidebar

PDF
Published: Dec 31, 2017
DOI: https://doi.org/10.11598/btb.2017.24.3.611
Keywords:
Bacteria, bioremediation, coastal, fertilizer, Indonesia, oil, optimal

Main Article Content

Yeti Darmayati
Research Center for Oceanography- Indonesian Institute of Sciences
Harpasis S. Sanusi
Tri Prartono
Dwi Andreas Santosa
Ruyitno Nuchsin

Abstract

Bioremediation, involving biostimulation and/or bioaugmentation, is a promising method to overcome oil spills in Cilacap coastal waters. Cilacap coastal area has high risk on oil pollution. This study investigated the stimulatory effect of nitrogen concentration, bacterial density and the composition of bacterial culture in enhancing oil degradation in this area. The applications of 4 different concentrations of Slow Release Fertilizer (SRF) and 2 different densities of bacterial cells in the form of single (RCO/B/08_008) and mixed culture were employed in microcosm experiments for 28 days. The efficacy of combining bacterial culture and fertilizer application in various concentrations was also tested. Oil degradation, bacterial growth and environmental parameters were monitored periodically during the experiments. The results showed that oil degradation rate was more influenced by nutrient concentration (biostimulation) than bacterial number or culture composition (bioaugmentation) added. The efficacy of biostimulation in degrading oil was better than that of bioaugmentation. Biostimulation increased oil degradation up to 6.4 times higher than the control. The optimum of fertilizer concentration added was 7.5 mg N/g (C:N ratio of 1,000:75), which increased depletion rate both in biostimulation-only and the combination of biostimulation with bioaugmentation up to 6.4 and 7.5 times higher than the control, respectively. It is suggested that bioremediation of oil-contaminated sandy beach in Cilacap would be optimal by employing a combination of Slow Release Fertilizer at concentration having C/N ratio = 1,000 : 75 and RCO/B/08_008 culture at density of 0.5 x 108 cells/mL (100% homology with Alcanivorax sp. TE-9).

Downloads

Download data is not yet available.

Article Details

How to Cite
Darmayati, Y., Sanusi, H. S., Prartono, T., Santosa, D. A., & Nuchsin, R. (2017). OPTIMIZATION BACTERIAL DENSITY AND FERTILIZER DOSAGE FOR BIOREMEDIATION OF OIL CONTAMINATED SANDY BEACH: A CASE OF CILACAP, INDONESIA. BIOTROPIA, 24(3), 182–191. https://doi.org/10.11598/btb.2017.24.3.611
Issue
Vol. 24 No. 3 (2017)
Section
Research Paper

Authors who publish with this journal agree with the following terms:

  1. Authors retain copyright and grant the journal right of first publication, with the work 1 year after publication simultaneously licensed under a Creative Commons attribution-noncommerical-noderivates 4.0 International License that allows others to share, copy and redistribute the work in any medium or format, but only where the use is for non-commercial purposes and an acknowledgement of the work's authorship and initial publication in this journal is mentioned.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).