GENETICS IDENTIFICATION OF SEA HOLLY (Acanthus ilicifolius) THROUGH DNA BARCODING FROM COASTAL CILACAP, CENTRAL JAVA, INDONESIA
Keywords:A. ilicifolius, DNA barcode, matK, rbcL, sea holly
AbstractSea holly (Acanthus ilicifolius) is an important true mangrove species commonly growing on wetlands at the river mouths and coastal areas. Very limited information is available on the molecular taxonomy of sea holly growing along the coasts of Cilacap, Central Java, Indonesia. The present study aimed to identify the sea holly in coastal Cilacap and to produce a reference library on the molecular characteristics of the species. The recently recorded species were utilized for the barcoding investigation. Genetic identification was evaluated through the rbcL and matK gene. Young leaf samples of A. ilicifolius were collected for DNA extraction, isolation and amplification using the rbcL and matK primer. The length of rbcL gene was 608 bp, and the matK gene was 970 bp.The evolutionary history was build using the Neighbor-Joining Method. The barcode sequences rbcL and matK were analyzed using BLAST and MULTALIN. The sequences were also submitted to NCBI.Â Genus Acanthus (Acanthaceae) and other genera were clustered in the same clade with high bootstrap value. The results indicated that locus of rbcL and matK gene cannot be used for species differentiation in Acanthus, however, these genes can be used for distinguishing the genus level within Acanthaceae.
LicenseAuthors who publish with this journal agree with the following terms:
- Authors retain copyright and grant the journal right of first publication, with the work 1 year after publication simultaneously licensed under a Creative Commons attribution-noncommerical-noderivatesÂ 4.0 InternationalÂ License that allows others to share, copy and redistribute the work in any medium or format,Â but only where the use is for non-commercial purposesÂ and an acknowledgement of the work's authorship and initial publication in this journal is mentioned.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).