ENHANCEMENT OF TOMATO GROWTH AND BIOCONTROL OF FUSARIUM SOLANI ROOT ROT DISEASE BY STREPTOMYCES ROCHEI BT02
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This study investigated the dual role of Streptomyces rochei BT02 in promoting tomato growth and suppressing Fusarium solani, the causative agent of tomato root rot disease. Various assays were conducted to evaluate the plant growth-promoting attributes of strain BT02 including indole-3-acetic acid (IAA) biosynthesis, phosphate solubilization, biofilm formation, and siderophore production. Under in vitro conditions, strain BT02 exhibited plant growth-promoting characteristics such as cellulose degradation, phosphate solubilization, IAA synthesis, biofilm formation, and siderophore production. Application of strain BT02 significantly improved tomato seed germination (26.7%), with enhanced sprout and radicle lengths and seed vigor (38.6%). Greenhouse experiments revealed substantial increases in plant height, leaf and branch numbers, inflorescence, and flower number, emphasizing the positive impact of strain BT02 on overall plant growth. The BT02 strain demonstrated antagonistic efficacy of 45.3 to 58.9% against Fusarium solani, as evidenced by the dual culture and agar well diffusion methods. Under greenhouse conditions, strain BT02 significantly reduced disease incidence and demonstrated control efficacy of 41.6% against Fusarium solani, highlighting its biocontrol potential. This study provides insights into the mechanisms employed by Streptomyces rochei BT02 to enhance plant growth and suppress root rot disease, paving the way for its application as a biocontrol agent in sustainable agriculture.
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Abbott WS. 1925. A Method of Computing the Effectiveness of an Insecticide. Journal of Economic Entomology. 18(2):265–267. doi:10.1093/jee/18.2.265a. DOI: https://doi.org/10.1093/jee/18.2.265a
de Andrade LA, Santos CHB, Frezarin ET, Sales LR, Rigobelo EC. 2023. Plant Growth-Promoting Rhizobacteria for Sustainable Agricultural Production. Microorganisms. 11(4). doi:10.3390/microorganisms11041088. DOI: https://doi.org/10.3390/microorganisms11041088
Azman N, Sijam K, Hata E, Othman R, Saud H. 2017. Screening of Bacteria as Antagonist against Xanthomonas oryzae pv. oryzae, the Causal Agent of Bacterial Leaf Blight of Paddy and as Plant Growth Promoter. Journal of Experimental Agriculture International. 16(4):1–15. doi:10.9734/jeai/2017/33697. DOI: https://doi.org/10.9734/JEAI/2017/33697
Bashan Y, Puente ME, Myrold DD, Toledo G. 1998. In vitro transfer of fixed nitrogen from diazotrophic filamentous cyanobacteria to black mangrove seedlings. FEMS Microbiology Ecology. 26(3):165–170. doi:10.1111/j.1574-6941.1998.tb00502.x. DOI: https://doi.org/10.1111/j.1574-6941.1998.tb00502.x
Berríos J, Illanes A, Aroca G. 2004. Spectrophotometric method for determining gibberellic acid in fermentation broths. Biotechnology Letters. 26(1):67–70. doi:10.1023/B:BILE.0000009463.98203.8b. DOI: https://doi.org/10.1023/B:BILE.0000009463.98203.8b
Bubici G. 2018. Streptomyces spp. as biocontrol agents against Fusarium species. CABI Reviews.:1–15. doi:10.1079/PAVSNNR201813050. DOI: https://doi.org/10.1079/PAVSNNR201813050
Dias MP, Bastos MS, Xavier VB, Cassel E, Astarita L V., Santarém ER. 2017. Plant growth and resistance promoted by Streptomyces spp. in tomato. Plant Physiology and Biochemistry. 118:479–493. doi:10.1016/j.plaphy.2017.07.017. DOI: https://doi.org/10.1016/j.plaphy.2017.07.017
Elshafie HS, Camele I. 2022. Rhizospheric Actinomycetes Revealed Antifungal and Plant-Growth-Promoting Activities under Controlled Environment. Plants. 11(14). doi:10.3390/PLANTS11141872. DOI: https://doi.org/10.3390/plants11141872
El-Sharkawy EES, Abdelrazik E. 2022. Biocontrol of Fusarium root rot in squash using mycorrhizal fungi and antagonistic microorganisms. Egyptian Journal of Biological Pest Control. 32(1):13. doi:10.1186/s41938-022-00513-x. DOI: https://doi.org/10.1186/s41938-022-00513-x
Jeger MJ, Viljanen-Rollinson SLH. 2001. The use of the area under the disease-progress curve (AUDPC) to assess quantitative disease resistance in crop cultivars. Theoretical and Applied Genetics. 102(1):32–40. doi:10.1007/s001220051615. DOI: https://doi.org/10.1007/s001220051615
Jog R, Nareshkumar G, Rajkumar S. 2012. Plant growth promoting potential and soil enzyme production of the most abundant Streptomyces spp. from wheat rhizosphere. Journal of Applied Microbiology. 113(5):1154–1164. doi:10.1111/j.1365-2672.2012.05417.x. DOI: https://doi.org/10.1111/j.1365-2672.2012.05417.x
Kanini GS, Katsifas EA, Savvides AL, Karagouni AD. 2013. Streptomyces rochei ACTA1551, an indigenous greek isolate studied as a potential biocontrol agent against fusarium oxysporum f.sp. lycopersici. BioMed Research International. 2013. doi:10.1155/2013/387230. DOI: https://doi.org/10.1155/2013/387230
Mazlan NA, Santhanam J, Zin NM, Santiago C. 2020. Effects of combination of phenazine-1-carboxylic acid from Streptomyces kebangsaanensis with amphotericin B on clinical Fusarium solani species. Malaysian Journal of Microbiology. 16(5):432–437. doi:10.21161/mjm.190603. DOI: https://doi.org/10.21161/mjm.190603
Moayedi G. 2010. Antagonistic Activities of Trichoderma spp . on Phytophthora Root Rot of Sugar Beet. Iran Agricultural Research. 29(2):21–37.
Nautiyal CS. 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters. 170(1):265–270. doi:10.1016/S0378-1097(98)00555-2. DOI: https://doi.org/10.1016/S0378-1097(98)00555-2
Nazari MT, Schommer VA, Braun JCA, dos Santos LF, Lopes ST, Simon V, Machado BS, Ferrari V, Colla LM, Piccin JS. 2023. Using Streptomyces spp. as plant growth promoters and biocontrol agents. Rhizosphere. 27:100741. doi:https://doi.org/10.1016/j.rhisph.2023.100741. DOI: https://doi.org/10.1016/j.rhisph.2023.100741
Nguyen HNT, Ha TTM, Luong TTD, Nguyen VP. 2023. Biological characteristics and ability of Streptomyces rochei BT02 to inhibit the Ralstonia solanacearum causing bacterial wilt disease. Ministry of Science and Technology, Vietnam. 65(7):67–72. doi:10.31276/vjst.65(7).67-72. DOI: https://doi.org/10.31276/VJST.65(7).67-72
O’Toole G, Kaplan HB, Kolter R. 2000. Biofilm formation as microbial development. Annual review of microbiology. 54:49–79. doi:10.1146/annurev.micro.54.1.49. DOI: https://doi.org/10.1146/annurev.micro.54.1.49
Ryu CM, Faragt MA, Hu CH, Reddy MS, Wei HX, Paré PW, Kloepper JW. 2003. Bacterial volatiles promote growth in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America. 100(8):4927–4932. doi:10.1073/pnas.0730845100. DOI: https://doi.org/10.1073/pnas.0730845100
Sasirekha B, Shivakumar S, Sullia SB. 2012. Statistical optimization for improved indole-3-acetic acid (iaa) production by pseudomonas aeruginosa and demonstration of enhanced plant growth promotion. Journal of Soil Science and Plant Nutrition. 12(4):863–873. doi:10.4067/s0718-95162012005000038. DOI: https://doi.org/10.4067/S0718-95162012005000038
Sharma C, Singh BP, Thakur N, Gulati S, Gupta S, Mishra SK, Panwar H. 2017. Antibacterial effects of Lactobacillus isolates of curd and human milk origin against food-borne and human pathogens. 3 Biotech. 7(1):31. doi:10.1007/s13205-016-0591-7. DOI: https://doi.org/10.1007/s13205-016-0591-7
Soytong K. 2010. Evaluation of Chaetomium-Biological Fungicide to Control Phytophthora Stem and Root Rot of Durian.
Srinivas V, Gopalakrishnan S, Kamidi JP, ... 2020. Effect Of Plant Growth-Promoting Streptomyces Sp. On Plant Growth And Yield Of Tomato And Chilli. Andhra Pradesh J Agril Sci. 6(2):65–70.
Sudha A, Durgadevi D, Archana S, Muthukumar A, Suthin Raj T, Nakkeeran S, Poczai P, Nasif O, Ansari MJ, Sayyed RZ. 2022. Unraveling the tripartite interaction of volatile compounds of Streptomyces rochei with grain mold pathogens infecting sorghum. Frontiers in Microbiology. 13(July):1–15. doi:10.3389/fmicb.2022.923360. DOI: https://doi.org/10.3389/fmicb.2022.923360
Thilagam R, Hemalatha N. 2019. Plant growth promotion and chilli anthracnose disease suppression ability of rhizosphere soil actinobacteria. Journal of Applied Microbiology. 126(6):1835–1849. doi:10.1111/jam.14259. DOI: https://doi.org/10.1111/jam.14259
Venkatramani M, Jayaprakashvel M. 2023. Biological Control of sheath blight disease in rice using Marine Actinomycetes (Streptomycetes rochei) AMET A311 strain. International Journal of Agricultural Sciences and Veterinary Medicine. 11(2):18–22. doi:10.25303/1102ijasvm018022. DOI: https://doi.org/10.25303/1102ijasvm018022
Wang S, Hu T, Jiao Y, Wei J, Cao K. 2009. Isolation and characterization of Bacillus subtilis EB-28, an endophytic bacterium strain displaying biocontrol activity against Botrytis cinerea Pers. Frontiers of Agriculture in China. 3(3):247–252. doi:10.1007/s11703-009-0042-x. DOI: https://doi.org/10.1007/s11703-009-0042-x
Woith E, Guerriero G, Hausman J-F, Renaut J, Leclercq CC, Weise C, Legay S, Weng A, Melzig MF. 2021. Plant Extracellular Vesicles and Nanovesicles: Focus on Secondary Metabolites, Proteins and Lipids with Perspectives on Their Potential and Sources. International journal of molecular sciences. 22(7). doi:10.3390/ijms22073719. DOI: https://doi.org/10.3390/ijms22073719
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