THE POSSIBILITY OF CONTROLLING SCLEROTIUM ROLFSII ON SOYBEAN (GLYCINE MAX) USING TRICHODERMA AND TEBUCONAZOLE*)
AbstractABSTRACT The possibility ofÂ controlling S.Â rolfsii on soybeanÂ (Glycine max) var. Rinjani using T. aureovirideÂ and TebuconazoleÂ underÂ fieldÂ conditions wasÂ studied. TheÂ experiment wasÂ conductedÂ atÂ theÂ experimentalÂ plotÂ of SEAMEO BIOTROP. The pathogen was mixed with the soil (2 kg/plot) 4 days before the inoculation of the antagonistÂ (2.25 kg/plot). The measurement of each plot was 2.5 x 6 m2. N, P and K (120 kg/ha) were applied at the same day with the inoculation of the pathogen. Soybean seeds were planted 7 days after the inoculation of the antagonist. The distance between plants and between plots were 20 and 40 cm, respectively. TheÂ fungicideÂ atÂ concentrationÂ ofÂ 100Â g/haÂ (inÂ vitroÂ concentration)Â andÂ 210Â g/haÂ (fieldÂ or recommended concentration) were applied using 2 methods, i.e. 1) spraying on the planting hole at the same day as the planting of soybean seeds, and 2) spraying on the soil surrounding the plants 7 days after planting. Soils that were neither inoculated with the antagonist nor the fungicide were used as controls. Three replications (3 plots) were used for each treatment (including the control). The results showed that the inoculation of the antagonist, the concentrations of the fungicide, and time of application gave very significant differences in the percentages of the plants infected by the pathogen and significant differences in seed production; while the interaction between the inoculation of the antagonist and the concentrations of the fungicide, between the concentrations of the fungicide and the time of application, and between the inoculation of the antagonist, the concentrations of the fungicide and the time of application did notÂ giveÂ significantÂ differencesÂ eitherÂ inÂ theÂ percentagesÂ ofÂ theÂ plantsÂ infectedÂ byÂ theÂ pathogenÂ orÂ seed production. The percentage of plants infected by the pathogen was lower on soil inoculated with the antagonist (31.6%) than on soil not inoculated with the antagonist (52.9%). The percentage of plants infected by the pathogen was lower on soil treated with the fungicide either at in vitro concentration (37.5%) or at field concentration (37.4%) than on the soil not treated
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