PRODUCTION AND CHARACTERIZATION OF BIOSURFACTANTS PRODUCED BY Pseudomonas aeruginosa B031 ISOLATED FROM A HYDROCARBON PHYTOREMEDIATION FIELD
Downloads
The biosurfactants are used by several industrial sectors such as petroleum, agriculture, food production, chemistry, cosmetics, and pharmaceuticals. Because of their hydrophobic and hydrophilic moieties, they have potency to reduce surface tension, interfacial tension between water-hydrocarbon systems, and low micelle concentration. Their characteristics strongly depend on the producer strain as well as on the medium composition, such as carbon and nitrogen sources. This study was conducted to investigate the influence of different sources of carbon (n-hexadecane, glycerol and glucose) and nitrogen (urea, NH4Cl and NaNO3) for the production of biosurfactants by a new strain of Pseudomonas aeruginosa B031 isolated from a rhizosphere of Paraserianthes falcataria L. Nielsen, a hardwood plant species at a phytoremediation field. The biosurfactant characteristics of the strain were evaluated, particularly its surface-active properties and potential to remove hydrocarbon. Glycerol was found to be the optimum carbon source, with rhamnose concentration, emulsification index, and critical micelle concentration (CMC) of 718 mg/L, 37%, and 35 mN/m, respectively. NaNO3 was observed as the optimum nitrogen source, with rhamnose concentration, emulsification index, and CMC of 290 mg/L, 30%, and 24 mN/m, respectively. These biosurfactants efficiently reduced surface tension of culture broth from 42 mN/m to 31 mN/m for the glycerol treatment and from 37 mN/m to 24 mN/m for the sodium nitrate treatment. The crude biosurfactants from the glycerol and sodium nitrate treatments also removed 87.5% and 84%, respectively, of crude oil from sand. These rates were higher than those of the chemical surfactants (SDS and Triton X-100). These findings indicate that the biosurfactants produced by the strain from both glycerol and NaNO3 treatments can efficiently decrease the interfacial tension of culture broth dilution and have a high emulsion index, thus hold promise in hydrocarbon bioremediation application.
Agbogidi MO, Dolor ED, Okechukwu EM. 2007. Evaluation of Tectona grandis (Linn.) and Gmelina arborea (Roxb.) for phytoremediation in crude oil contaminated soils. Agric Conspec Sci 72(2):149-52.
Al-Bahry SN, Al-Wahaibi YM, Elshafie AE, Al-Bemani AS, Joshi SJ, Al-Makhmari HS, Al-Sulaimani HS. 2013. Biosurfactant production by Bacillus subtilis B20 using date molasses and its possible application in enhanced oil recovery. Int Biodeterior Biodegradation 81:141-6.
Amani H, Müller MM, Syldatk C, Hausmann R. 2013. Production of microbial rhamnolipid by Pseudomonas aeruginosa MM1011 for ex situ enhanced oil recovery. Appl Biochem Biotechnol 170:1080-93.
Aparna A, Srinikethan G, Smitha H. 2012. Production and characterization of biosurfactant produced by a novel Pseudomonas sp. 2B. Colloids Surf B 95:23-9.
Arslan G, Kipcak E, Dirik C, Aktu T, Peker S. 2017. Surface adsorption and spontaneous aggregation of rhamnolipid mixtures in aqueous solutions. Colloids Surfaces A Physicochem Eng As 519:125-36.
Barakat KM, Hassan SWM, Darwesh OM. 2017. Biosurfactant production by haloalkaliphilic Bacillus strains isolated from Red Sea, Egypt. Egypt J Aquat Res 43:205-11.
Brenner DJ, Krieg NR, Staley JR. 2005. Bergey's manual systematic bacteriology. New York (US): Springer.
Burgos-Díaz C, Pons R, Teruel JA, Aranda FJ, Ortiz A, Manresa AM, Marqués J. 2013. The production and physicochemical properties of a biosurfactant mixture obtained from Sphingobacterium detergens. Colloid Interface Sci 394:368-79.
Calvo C, Manzanera M, Silva-Castro GA, Uad I, González-López J. 2009. Application of bioemulsifiers in soil oil bioremediation processes. Future prospects Sci Total Environ 407:3634-40.
Cheng F. 2013. Characterization of a blend-biosurfactant of glycolipid and lipopeptide produced by Bacillus subtilis TU2 isolated from underground oil-extraction wastewater. J Microbiol Biotechnol 23:390-6.
Costa SGVAO, Nitschke M, Lépine F, Déziel E, Contieroa J. 2010. Structure, properties and applications of rhamnolipids produced by Pseudomonas aeruginosa L2-1 from cassava wastewater. Process Biochem 45:1511-6.
Cristaldi A, Conti GO, Jho EH, Zuccarello P, Grasso A, Copat C, Ferrante M. 2017. Phytoremediation of contaminated soils by heavy metals and PAHs. A brief review. Environ Technol Innov 8:309-26.
Deepika KV, Kalam S, Sridhar PR, Podile AR, Bramhachari PV. 2016. Optimization of rhamnolipid biosurfactant production by mangrove sediment bacterium KVD-HR42 using Pseudomonas aeruginosa response surface methodology. Biocatal Agric Biotechnol 5:38-47.
Fatima K, Imran A, Amin I, Khan QM, Afzal M. 2018. Successful phytoremediation of crude-oil contaminated soil at an oil exploration and production company by plant-bacterial synergism. Int J Phytoremediation 20:675-81.
Geetha SJ, Banat IM, Joshi SJ. 2018. Biosurfactants: Production and potential applications in microbial enhanced oil recovery (MEOR). Biocat Agric Biotechnol 14:23-32.
Guo M, Gong Z, Miao R, Rookes J, Cahill D, Zhuang J. 2017. Microbial mechanisms controlling the rhizosphere effect of ryegrass on degradation of polycyclic aromatic hydrocarbons in an aged-contaminated agricultural soil. Soil Biol Biochem 113:130-42.
Hassanshahian M, Emitazi G. 2008. Investigation of alkane biodegradation using the microtiter plate method and correlation between biofilm formation, biosurfactant production and crude oil biodegradation. Int Biodeterior Biodegrad 62:170-8.
Hou J, Liu W, Wang B, Wang Q, Luo Y, Franks AE. 2015. PGPR enhanced phytoremediation of petroleum-contaminated soil and rhizosphere microbial community response. Chemosphere 138:592-8.
İkizler B, Arslan G, Kipcak E, Dirik Ç, Çelenk D, Aktuğlu T, ... Peker S. 2017. Surface adsorption and spontaneous aggregation of rhamnolipid mixtures in aqueous solutions. Colloids Surfaces A Physicochem Eng Aspects 519:125-36.
Janek T, Lukaszewicz M, Rezanka M, Krasowska A. 2010. Isolation and characterization of two new lipopeptide biosurfactants produced by Pseudomonas fluorescens BD5 isolated from water from Arctic Archipelago of Svalbard. Biores Technol 101:6118-23.
Joshi PA, Shekhawat DB. 2014. Screening and isolation of biosurfactant producing bacteria from petroleum-contaminated soil. Eur J Exp Biol 4:164-9.
Khan S, Afzal M, Iqbal S, Khan QM. 2013. Plant–bacteria partnerships for the remediation of hydrocarbon-contaminated soils. Chemosphere 90:1317-32.
Kłosowska-Chomiczewska IE, Mędrzycka K, Hallmann E, Karpenko E, Pokynbroda T, Macierzanka A, Jungnickel C. 2017. Rhamnolipid CMC prediction. J Colloid Interface Sci 488:10-9.
Kong Q, Wang Z, Niu P, Miao M. 2016. Greenhouse gas emission and microbial community dynamics during simultaneous nitrification and denitrification process. Bioresour Technol 210:94-100.
Kryachko Y, Semler D, Vogrinetz J, Lemke M, Links MG, Mccarthy EL, ... Hemmingsen SM. 2016. Enrichment and identification of biosurfactant-producing oil field microbiota utilizing electron acceptors other than oxygen and nitrate. J Biotechnol 231:9-15.
Lai C, Huang YC, Wei YH, Chang JS. 2009. Biosurfactant-enhanced removal of total petroleum hydrocarbons from contaminated soil. J Hazard Mater 167:609-14.
Liu Q, Lin J, Wang W, Huang H, Li S. 2015. Production of surfactin isoforms by Bacillus subtilis BS-37 and its applicability to enhanced oil recovery under laboratory conditions. Biochem Eng J 93:31-7.
Ma KY, Sun MY, Dong W, He CQ, Chen FL, Ma YL. 2016. Effects of nutrition optimization strategy on rhamnolipid production in a Pseudomonas aeruginosa strain DN1 for bioremediation of crude oil. Biocatal Agric Biotechnol 6:144-51.
Ma Z, Liu J, Dick RP, Li H, Shen D, Gao Y, ... Ling W. 2018. Rhamnolipid influences biosorption and biodegradation of phenanthrene by phenanthrene-degrading strain Pseudomonas sp. Ph6. Environ Poll 240:359-67.
Mnif I, Ghribi D. 2015. High molecular weight bioemulsifiers, main properties and potential environmental and biomedical applications. World J Microbiol Biotechnol 31:691-706.
Monteiro MR, Kugelmeier CL, Pinheiro RS, Batalha MO, da Silva César A. 2018. Glycerol from biodiesel production: Technological paths for sustainability. Renew Sustain Energy Rev 88:109-22.
Moya RI, Tsaousi K, Rudden M, Marchant R, Alameda EJ, García RM, Banat IM. 2015. Rhamnolipid and surfactin production from olive oil mill waste as sole carbon source. Bioresour Technol 198:231-6.
Müller MM, Kügler JH, Henkel M, Gerlitzki M, Hörmann B, Pöhnlein M, ... Hausmann R. 2012. Rhamnolipids – Next Generation Surfactants? J Biotechnol 162:366-80.
Pacwa-Płociniczak M, Płaza GA, Piotrowska-Seget Z, Cameotra SS. 2011. Environmental applications of biosurfactants: Recent advances. Int J Mol Sci 12:633-54.
Panjiar N, Sachan SG, Sachan A. 2015. Screening of bioemulsifier-producing microorganisms isolated from oil-contaminated sites. Ann Microbiol 65:753-64.
Patowary R, Patowary K, Chandra Kalita M, Deka S. 2018. Application of biosurfactant for enhancement of bioremediation process of crude oil contaminated soil. Int Biodeterior Biodegradation 129:50-60.
Petrikov K, Delegan Y, Surin A, Ponamoreva O, Puntus I, Filonov A, Boronin A. 2013. Glycolipids of Pseudomonas, Rhodococcus and oil-degrading bacteria used in bioremediation preparations: Formation and structure. Process Biochem 48:31-5.
Rizzo MG, Chines V, Franco D, Nicolò MS, Guglielmino SPP. 2017. The role of glutamine in Pseudomonas mediterranea in biotechnological processes. New Biotechnol 37:144-51.
Rufino RD, Luna JM, Sarubbo LA, Rodrigues LRM, Teixeira JAC, Campos-Takaki GM. 2011. Antimicrobial and anti-adhesive potential of a biosurfactant Rufisan produced by Candida lipolytica UCP 0988. Colloids Surf B 84:1-5.
Sakthipriya N, Doble M, Sangwai JS. 2015. Biosurfactant from Pseudomonas species with waxes as carbon source – Their production, modeling and properties. J Ind Eng Chem 31:100-11.
Seedevi P, Moovendhan M, Sudharsan S, Sivasankar P, Sivakumar L, Vairamani S, Shanmugam A. 2018. Isolation and chemical characteristics of rhamnose enriched polysaccharide from Grateloupia lithophila. Carbohydr Polym 195:486-94.
Shavandi M, Mohebali G, Haddadi A, Shakarami H, Nuhi A. 2011. Emulsification potential of a newly isolated biosurfactant-producing bacterium, Rhodococcus sp. strain TA6. Colloids Surf B 82:477-82.
Sodagari M, Invally K, Ju LK. 2018. Maximize rhamnolipid production with low foaming and high yield. Enzyme Microb Technol 110:79-86.
Thavasi R. 2011. Microbial biosurfactants: From an environmental application point of view. J Bioremediat Biodegrad 2:104-12.
Thavasi R, Sharma S, Jayalakshmi S. 2011. Evaluation of screening methods for the isolation of biosurfactant producing marine bacteria. J Pet Environ Biotechnol S1:1-6.
Thorsen MK, Brandt KK, Nybroe O. 2013. Abundance and diversity of culturable Pseudomonas constitute sensitive indicators for adverse long-term copper impacts in soil. Soil Biol Biochem 57:933-5.
Varjani SJ. 2017. Microbial degradation of petroleum hydrocarbons. Bioresour Technol 223:277-86.
Varjani SJ, Upasani VN. 2016. Carbon spectrum utilization by an indigenous strain of Pseudomonas aeruginosa NCIM 5514: Production, characterization and surface-active properties of biosurfactant. Bioresour Technol 221:510-6.
Villalobos M, Avila-Forcada AP, Gutierrez-Ruiz ME. 2008. Improved gravimetric method to determine total petroleum hydrocarbons in contaminated soils. Water Air Soil Pollut 194:151-61.
Vinogradov E, Sadovskaya I, Grard T, Chapot-Chartier MP. 2016. Structural studies of the rhamnose-rich cell wall polysaccharide of Lactobacillus casei BL23. Carbohydr Res 435:156-61.
Vijayakumar S, Saravanan V. 2015. Biosurfactants-types, sources and applications. Research J Microbiology 10:181-92.
Yenn R, Borah M, Boruah HD, Roy AS, Baruah R, Saikia N, Tamuli AK. 2014. Phytoremediation of abandoned crude oil contaminated drill sites of Assam with the aid of a hydrocarbon-degrading bacterial formulation. Int J Phytoremediation 16:909-25.
Zdziennicka A, Jańczuk B. 2018. Wetting and adhesion properties of rhamnolipid and surfactin. Int J Adhesion Adhesives 84:275-82.
Copyright (c) 2018 BIOTROPIA - The Southeast Asian Journal of Tropical Biology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Authors who publish with this journal agree with the following terms:
- Authors retain copyright and grant the journal right of first publication, with the work 1 year after publication simultaneously licensed under a Creative Commons attribution-noncommerical-noderivates 4.0 International License that allows others to share, copy and redistribute the work in any medium or format, but only where the use is for non-commercial purposes and an acknowledgement of the work's authorship and initial publication in this journal is mentioned.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
