CHARACTERIZATION AND POLYPHASIC IDENTIFICATION OF NOVEL RHIZOBACTERIA STRAIN ISOLATED FROM SAND DUNES ECOSYSTEM
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The coastal sand dune ecosystem at the Parangtritis Coast of Yogyakarta, Indonesia has unique characteristics such as low moisture sandy soil, high salinity and low nutrient content. Fimbristylis cymosa is one of the plant species having the capability to survive in that unique ecosystem. In this study, rhizobacteria isolated from the rhizosphere of F. cymosa were isolated to be further analyzed on their phosphate solubilizing and antagonistic properties against Fusarium oxysporum which cause the Wilt disease. The isolates of Phosphate Solubilizing Rhizobacteria (PSR) having the most potential capabilities were then polyphasically identified based on phenotypic and genotypic characters followed by 16S rDNA sequencing. The results showed that four PSR isolates (I8, I11, I12 and I24) have high phosphate dissolution indices. The highest indices were observed in isolates I11 (3.08) and I12 (3.44), respectively. Analysis of the dual plate experiments for PSR I11 and PSR I12 isolates against the growth of F. oxysporum also showed quite high inhibitory activities, i.e., isolate PSR I11 was 42.40%, while isolate PSR I12 was 42.08%. The two isolates were polyphasically identified as Burkholderia dolosa. This study clearly showed that PSR I11 and PSR I12 isolates are very potential and prospective to be used as marginal land inoculants and as providers of phosphorus. This study also showed that the isolates are useful as biocontrol agents against F. oxysporum in plants.
Ahluwalia O, Singh PC, Bhatia R. 2021. A review on drought stress in plants: Implications, mitigation and the role of plant growth promoting rhizobacteria. Res Environ Sustain 5:100032.
Ávila AC, Ochoa J, Proaño K, Martínez MC. 2019. Jasmonic acid and nitric oxide protect naranjilla (Solanum quitoense) against infection by Fusarium oxysporum f. sp. quitoense by eliciting plant defense responses. Physiol Mol Plant Pathol 106:129–136.
Babu AN, Jogaiah S, Ito S, Nagaraj AK, Tran LP. 2015. Improvement of growth, fruit weight and early blight disease protection of tomato plants by rhizosphere bacteria correlated with their beneficial traits and induced biosynthesis of antioxidant peroxidase and polyphenol oxidase. Plant Sci 231:62–73.
Bauernfeind A, Schneider I, Jungwirth R, Roller C. 1999. Discrimination of Burkholderia multivorans and Burkholderia vietnamiensis from Burkholderia cepacia genomovars I, III, and IV by PCR. J Clin Microbiol 37(5):1335–1339.
Behera BC, Yadav H, Singh SK, Sethi BK, Mishra RJ, Kumari S, Thatoi H. 2017. Alkaline phosphatase activity of a phosphate-solubilizing Alcaligenes faecalis isolated from mangrove soil. Biotechnol Res Innov 1:101–110.
Blanco-Vargas A, Rodríguez-Gacha LM, Sanchez-Castro N, Garzon-Jaramillo R, Pedroza-Camacho LD, Poutou-Pinales RA, Pedroza-Rodríguez AM. 2020. Phosphate-solubilizing Pseudomonas sp. and Serratia sp. co-culture for Allium cepa L. growth promotion. Heliyon 6:e05218.
Brenner DJ, Krieg NR, Staley JR. 2005. Bergey’s Manual of Systematic Bacteriology. New York (US): Springer. p. 52–150.
Campos CA, Suarez MG, Laborde J. 2020. Analyzing vegetation cover-induced organic matter mineralization dynamics in sandy soils from tropical dry coastal ecosystems. Catena 185:104264.
Cherchali A, Boukhelata N, Kaci Y, Abrous-Belbachir O, Djebbar R. 2019. Isolation and identification of a phosphate-solubilizing Paenibacillus polymyxa strain GOL 0202 from durum wheat (Triticum durum Desf.) rhizosphere and its effect on seedling morphophysiological parameters. Biocatal Agric Biotechnol 19:101087.
Chowdhury SK, Majumdar S, Mandal V. 2020. Application of Bacillus sp. LBF-01 in Capsicum annuum plant reduces fungicide use against Fusarium oxysporum. Biocatal Agric Biotechnol 27:101714.
Cui W, He P, Munir S, Li X, Li Y, He Y. 2019. Efficacy of plant growth promoting bacteria Bacillus amyloliquefaciens B9601-Y2 for biocontrol of southern corn leaf blight. Biol Control 139:104080.
da Silva K, Cassetari AD, Lima AS, Brandt ED, Pinnock E, Vandamme P, Moreira FMD. 2012. Diazotrophic Burkholderia species isolated from the Amazon region exhibit phenotypical, functional and genetic diversity. Syst Appl Microbiol 35:253–262.
de Castilho CL, Longoni L, Sampaio J, Lisboa BB, Vargas LK, Beneduzi A. 2020. The rhizosphere microbiome and growth-promoting rhizobacteria of the Brazilian juçara palm. Rhizosphere 15:100233.
De Smet B, Mayo M, Peeters C, Zlosnik JE, Spilker T, Hird TJ, Vandamme P. 2015. Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources. Int J Syst Evol Microbiol 65(7):2265–2271.
Guleria S, Walia A, Chauhan A, Shirkot CK. 2016. Molecular characterization of alkaline protease of Bacillus amyloliquefaciens SP1 involved in biocontrol of Fusarium oxysporum. Int J Food Microbiol 232:134–143.
Haile D, Mekbib F, Assefa F. 2016. Isolation of phosphate solubilizing bacteria from white lupin (Lupinus albus L.) rhizosphere soils collected from Gojam, Ethiopia. J Fertil Pestic 7(2):1000172.
Hillis DM, Bull JJ. 1993. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Syst Biol 42(2):182–192.
Hossain MI, Mizan MFR, Ashrafudoulla M, Nahar S, Joo HJ, Jahid IK, Ha SD. 2020. Inhibitory effects of probiotic lactic acid bacteria isolated from kimchi against Listeria monocytogenes biofilm. LWT–Food Sci Technol 118:108864.
Jangir M, Pathak R, Sharma S, Sharma S. 2018. Biocontrol mechanisms of Bacillus sp. isolated from tomato rhizosphere against Fusarium oxysporum f. sp. lycopersici. Biol Control 123:60–70.
Joe MM, Deivaraj S, Abitha Benson A, Henry AJ, Narendrakumar G. 2018. Soil extract calcium phosphate media for screening of phosphate-solubilizing bacteria. Agric Nat Resour 52:305–308.
Karthika S, Midhun SJ, Jisha MS. 2020. A potential antifungal and growth-promoting bacterium Bacillus sp. KTMA4 from tomato rhizosphere. Microb Pathog 142:104049.
Khedhera SB, Mejdoub-Trabelsi B, Tounsi S. 2021. Biological potential of Bacillus subtilis V26 for control of Fusarium wilt and tuber dry rot on potato and promotion of plant growth. Biol Control 152:104444.
Kumar H, Dubey RC, Maheshwari DK. 2017. Seed-coating fenugreek with Burkholderia rhizobacteria enhances yield and combats Fusarium wilt. Rhizosphere 3:92–99.
Lau ET, Tani A, Khew CY, Chua YQ, Hwang SS. 2020. Plant growth-promoting bacteria as potential bio-inoculants and biocontrol agents to promote black pepper cultivation. Microbiol Res 240:126549.
LiPuma JJ, Dulaney BJ, McMenamin JD, Whitby PW, Stull TL, Coenye T, Vandamme P. 1999. Development of rRNA-based PCR assays for identification of Burkholderia cepacia complex isolates. J Clin Microbiol 37(10):3167–3170.
Liu Z, Li YC, Zhang S, Fu Y, Fan X, Patel JS, Zhang M. 2015. Characterization of phosphate-solubilizing bacteria isolated from calcareous soils. Appl Soil Ecol 96:217–224.
Ludueña SLM, Anzuay MS, Angelini JG, McIntosh M, Becker A, Rupp O, Taurian T. 2018. Serratia sp. S119: A potential biofertilizer for peanut and maize. Appl Soil Ecol 126:107–112.
Mahdavi P, Bergmeier E. 2016. Plant functional traits and diversity in sand dune ecosystems across biogeographic regions. Acta Oecol 74:37–45.
Mardad IA, Serrano A, Soukri A. 2013. Solubilization of inorganic phosphate and organic acid production by bacteria from Moroccan phosphate deposits. Afr J Microbiol Res 7(8):626–635.
Nautiyal CS. 1999. An efficient microbiological growth medium for screening phosphate-solubilizing microorganisms. FEMS Microbiol Lett 170:265–270.
Panda B, Rahman H, Panda J. 2016. Phosphate solubilizing bacteria from acidic soils and antagonism against fungal pathogens. Rhizosphere 2:62–71.
Pastore G, Kernchen S, Spohn M. 2020. Microbial solubilization of silicon and phosphorus from bedrock. Soil Biol Biochem 151:108050.
Paul D, Sinha SN. 2013. Isolation of phosphate solubilizing bacteria from river water. Adv Appl Sci Res 4:409–412.
Rasool A, Mir MI, Zulfajri M, Hanafiah MM, Unnisa SA, Mahboob M. 2021. Plant growth promoting and antifungal traits of rhizobacteria from saffron rhizosphere. Microb Pathog 150:104734.
Rasul M, Yasmina S, Zubaira M, Mahreena N, Yousaf S, Arifa M, Mirza MS. 2019. Phosphate solubilizers as antagonists for bacterial leaf blight with improved rice growth. Biol Control 136:103997.
Rasul M, Yasmin S, Yahya M, Breitkreuz C, Tarkka M, Reitz T. 2021. Wheat growth-promoting traits of Ochrobactrum and Pantoea species. Microbiol Res 246:126703.
Royse DJ, Ries SM. 1978. Influence of fungi isolated from peach twigs on pathogenicity of Cytospora cincta. Phytopathology 68(4):603–607.
Sahandi MA, Mehrafarin A, Badi HN, Khalighi-Sigaroodi F, Sharifi M. 2019. Improving growth and antioxidant characteristics of peppermint by phosphate-solubilizing bacteria. Ind Crops Prod 141:111777.
Santos-Torres M, Romero-Perdomo F, Mendoza-Labrador J, Gutierrez AY, Vargas C, Castro-Rincon E, Estrada-Bonilla GA. 2021. Genomic and phenotypic analysis of rock phosphate-solubilizing rhizobacteria. Rhizosphere 17:100290.
Schlaberg R, Simmon KE, Fisher MA. 2012. A systematic approach for discovering novel clinically relevant bacteria. Emerg Infect Dis 18(3):422–430.
Simonetti E, Roberts IN, Montecchia MS, Gutierrez-Boem FH, Gomez FM, Ruiz JA. 2018. A novel Burkholderia ambifaria strain degrading fusaric acid. Microbiol Res 206:50–59.
Srinivas C, Devi DM, Murthy KN, Mohan CD, Lakshmeesha TR, Singh BP, Srivastava RK. 2019. Fusarium oxysporum f. sp. lycopersici: Biology to diversity. Saudi J Biol Sci 26:1315–1324.
Tagele SB, Kim SW, Lee HG, Kim HS, Lee YS. 2018. Effectiveness of multi-trait Burkholderia contaminans KNU17BI1. Microbiol Res 214:8–18.
Tapia-García EY, Hernández-Trejo V, Guevara-Luna J, Rojas-Rojas FU, Arroyo-Herrera I, Meza-Radilla G, Santos PE. 2020. Plant growth-promoting bacteria from wild legume nodules. Microbiol Res 239:126522.
Teymouri M, Akhtari J, Karkhane M, Marzban A. 2016. Phosphate solubilization activity of rhizobacteria in mangrove forest. Biocatal Agric Biotechnol 5:168–172.
Valetti L, Liliana IL, Fabra A. 2018. Growth promotion of rapeseed by phosphate solubilizing bacteria. Appl Soil Ecol 132:1–10.
Vanlaere E, LiPuma JJ, Baldwin A, Henry D, De Brandt E, Mahenthiralingam E, Vandamme P. 2008. Novel Burkholderia species within the Burkholderia cepacia complex. Int J Syst Evol Microbiol 58:1580–1590.
Viallard V, Poirier I, Cournoyer B, Haurat J, Wiebkin S, Ophel-Keller K, Balandreau J. 1998. Burkholderia graminis sp. nov. Int J Syst Bacteriol 48:549–563.
Widane KA, Retnaningrum E. 2018. Kemampuan bakteri pelarut fosfat dan antipatogen Fusarium oxysporum dari gumuk pasir. Final Research Project. Yogyakarta (ID): Universitas Gadjah Mada.
Yang F, Sui L, Tang C, Li J, Cheng K, Xue Q. 2021. Sustainable advances on phosphorus utilization via biochar and humic substances. Sci Total Environ 768:145106.
Yarza P, Sproer C, Swiderski J, Mrotzek N, Spring S, Tindall BJ, Rossello-Mora R. 2013. Sequencing orphan species initiative (SOS). Syst Appl Microbiol 36(1):69–73.
Zhang X, Zhou Y, Li Y, Fu X, Wang Q. 2017. Screening and characterization of endophytic Bacillus for biocontrol of grapevine downy mildew. Crop Prot 96:173–179.
Zhao K, Penttinen P, Zhang X, Ao X, Liu M, Yu X, Chen Q. 2014. Maize rhizosphere hosts plant growth-promoting Burkholderia cepacia. Microbiol Res 169:76–82.
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