PRODUCTION OF MALTOOLIGOSACCHARIDES FROM HUTAN JATI VARIETY CULTIVAR TACCA (Tacca leontopetaloides) STARCH
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This research aimed to extract and characterize the physicochemical properties of starch from Tacca tuber, to determine the optimum conditions for enzymatic hydrolysis to produce maltooligosaccharides, and to analyze the character of these maltooligosaccharides. The analysis was conducted by calculating the amount of reducing sugar, total sugar, and the degree of polymerization, and by using the TLC (Thin Layer Chromatography) and HPLC (High-Performance Liquid Chromatography) analyses. The Hutan Jati variety cultivar of Tacca was selected from three Tacca variety cultivars (Hutan Jati, Pulau Katang, and Gunung Batur) to produce maltooligosaccharides by enzymatic hydrolysis of crude Bacillus sp. α-amylase. The optimum conditions for the enzymatic hydrolysis of Hutan Jati variety cultivar Tacca starch for the production of maltooligosaccharides were obtained at a substrate concentration of 3% (w/v) and a ratio of enzyme and substrate at 6 hours incubation time. From 250 mL of fresh hydrolysate, 34.49 grams of powdered maltooligosaccharide were produced. The TLC and HPLC results showed a similar yield of both the liquid and powdered maltooligosaccharides with maltose, maltotriose, and maltotetraose as the main products. Considering its physicochemical characteristics and the product of its maltooligosaccharides, the starch from the tuber of Hutan Jati variety cultivar Tacca possessed strong potential for the future production of maltooligosaccharides, particularly maltotriose and maltotetraose, in food industries.
Aiyer PV. 2005. Amylases and their applications. J Biotechnol 4(13):1525-9.
Bal E, Pinar O, Kazan D, Öztürk HÜT, Sayar AA. 2016. Immobilization of α-amylase from Bacillus subtilis SDPI isolated from the rhizosphere of Acacia. J Elect of Biology.
AOAC. 1990. Official methods of analysis, 15th ed. Arlington (VA, US): Association of Official Analytical Chemists.
Bernfeld P. 1955. Amylase α- and β-methods. Enzymol:149-58.
Crittenden RG, Playne MJ. 1996. Production, properties and applications of food grade oligosaccharides. Trends Food Sci Technol.
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugar and related substances. J Anal Chem 28:350-6.
Hayashi T, Akiba T, Horikoshi K. 1988. Properties of new alkaline maltohexaose-forming amylases. Appl Microbiol Biotechnol.
Hwang SY, Nakashima K, Okai N, Okazaki F, Miyake M, Harazono K, ... Kondo A. 2013. Thermal stability and starch degradation profile of α-amylase from Streptomyces avermitilis. Biosci Biotechnol Biochem.
Jana M, Chiranjit MB, Saptadip SB, Bikos RPB, Syed SIC, Prodeep KDM, Keshab CM. 2013. Salt-independent thermophilic α-amylase from Bacillus megaterium VUMBI 09: An efficacy testing for preparation of maltooligosaccharide. Industrial Crops Products 41:1386-91.
Kanpiengjai A, Lumyong S, Nguyen TH, Haltrich D, Khanongnuch C. 2015. Characterization of a maltose-forming amylase from an amylolytic lactic acid bacterium Lactobacillus plantarum S21. J Mol Catal B Enzym 120:11-18.
Kandra L, Gyemant G, Liptak A. 2002. Action pattern of α-amylases on modified maltooligosaccharides. J Biol Chem.
Kashiwagi N, Miyake M, Hirose S, Sota M, Ogino C, Kondo A. 2014. Cloning and starch degradation profile of maltotriose-producing amylases from Streptomyces species. Biotechnol Lett 36(11):2311-7.
Lee JW, Kwon TO, Moon IS. 2003. Adsorption of monosaccharides, disaccharides, and maltooligosaccharides on activated carbon for separation of maltopentaose. Biotechnol Bioprocess Eng 8(1):47-53.
Li Z, Wu J, Zhang B, Wang F, Ye X, Huang Y, ... Cui Z. 2015. AmyM, a novel maltohexaose-forming α-amylase from Corallococcus sp. strain EGB. Appl Environ Microbiol 81(6).
Maalej H, Ayed HB, Bellaaj OG, Nasri M, Hmidet N. 2014. Production and biochemical characterization of a high maltotetraose (G4) producing amylase from Pseudomonas stutzeri AS22. BioMed Research International.
Murakami S, Nagasaki H, Nishimoto R, Shigematu J, Umesaki S, Takenaka R. 2008. Purification and characterization of five alkaline, thermotolerant and maltotetraose-producing α-amylases from Bacillus halodurans MS-2-5, and production of recombinant enzymes in Escherichia coli. Enzyme Microb Technol 43:321-8.
Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. J Anal Chem 31:426-8.
Olesen LD, Pedersen LH, Larsen KL. 2000. Suitability and limitations of methods for characterization of activity of maltooligosaccharide-forming amylases. Carbohydr Res 329:109-19.
Pan S, Ning D, Junyan R, Zhengbiao G, Caiming L, Yan H, ... Zhaofeng L. 2017. Maltooligosaccharide-forming amylase: Characteristics, preparation, and application. Biotechnol Advances 35:619-32.
Rastall RA. 2010. Functional oligosaccharides: Application and manufacture. Annu Rev Food Sci Technol 1:305-39.
Rastall RA, Maitin V. 2002. Prebiotics and synbiotics: Towards the next generation. Curr Opin Biotechnol 13:490-6.
Rahmani N, Yopi, Andriani A, Prima A. 2011. Production and characterization of amylase enzyme from marine bacteria. Proceedings of the 2nd International Seminar on Chemistry for a Better Future. p. 255-9.
Rahmani N, Rohanah, Sukarno, Andriani A, Yopi. 2013. Production of maltooligosaccharides from black potato (Coleus tuberosus) starch by α-amylase from a marine bacterium (Brevibacterium sp.). Microbiol Indonesia.
Rahmani N, Andriani A, Yopi, Hartati S. 2015. Production of maltooligosaccharide from cassava cultivar Kuning. J Penelitian Pascapanen Pertanian.
Rahmani N, Andriani A, Hartati S, Yopi. 2016. Enzymatic hydrolysis of the FEC 25 and ROTI cassava starch (Manihot esculenta) varieties by α-amylase from a marine bacterium (Brevibacterium sp.). J Teknologi Indonesia 39(3):150-7.
Sowbhagya CM, Bhattacharya KR. 1979. Simplified determination of amylose in milled rice. Starch 31:159-63.
Ukpabi UJ, Ukerye E, Olojede AO. 2009. Raw material potentials of Nigerian wild Polynesian arrowroot (Tacca leontopetaloides) tubers and starch. J Food Technol.
Yang CH, Liu WH. 2004. Purification and properties of a maltotriose-producing α-amylase from Thermoanaerobacterium fusca. Enzyme Microb Technol 35:254-60.
Yang CH, Liu WH. 2007. Cloning and characterization of a maltotriose-producing α-amylase gene from Thermobifida fusca. J Ind Microbiol Biotechnol 34:325-30. doi:10.1007/s10295-006-0200-6.
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