ISOLATION OF ENDOPHYTIC FUNGI FROM THE COASTAL PLANT TERONG PUNGO (Solanum sp.) AND ITS ANTIBACTERIAL ACTIVITY AGAINST ORAL PATHOGENIC BACTERIA
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Coastal plant locally known as terong pungo (Solanum sp.) was used by Aceh community as traditional medicine for toothache. The objectives of this study were to isolate endophytic fungi from the leaves of terong pungo and determine the antibacterial activity of the fungi against bacteria causing dental caries and infection. Eight endophytic fungi were isolated. Antagonism test among the isolates was performed to select the most dominant fungus. TP6 was the selected fungus based on the antagonism assay. Crude extracts of the fungus were macerated from the culture broth using ethyl acetate. The strongest antibacterial activity of the extracts was obtained when the fungus was at 12 days of cultivation. The fungal crude extract was strongly active against Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa with inhibition zone diameter of 20, 21 and 23 mm, respectively.
Al-Bayati FA, Sulaiman KD. 2007. In vitro antimicrobial activity of Salvadora persica L. extracts against some isolated oral pathogens in Iraq. Turkish Journal of Biology 32:57–62.
Arnold AE, Mejia LC, Kyllo D, Rojas EI, Maynard Z, Robbins N, Herre EA. 2003. Fungal endophytes limit pathogen damage in a tropical tree. PNAS 100(26):15649–54.
Barnett HL, Hunter HH. 1972. Illustrated Genera of Imperfect Fungi. Minneapolis (US): Burgers Company. 218 p.
Bean R, Straif K, Grosse Y, Secretan B, El Ghissassi F, Bouvard V. 2007. Carcinogenicity of alcoholic beverages. Lancet Oncology 8:292–3.
Demain AL, Fang A. 2000. The natural functions of secondary metabolites. In: Scheper T, editor. Advances in Biochemical Engineering Biotechnology. Vol. 69. Berlin (DE): Springer-Verlag. p. 20–9.
Gandjar I, Samson RA, Vermeulen, Oetari A, Santoso I. 2000. Pengenalan Kapang Tropik Umum. Jakarta (ID): Yayasan Obor Indonesia. p. 33–40.
Guo LD, Hyde KD, Liew ECY. 2000. Identification of endophytic fungi from Livistona chinensis based on morphology and rDNA sequences. New Phytologist 147:617–30.
Hardjito L. 2008. Antibacterial and topoisomerase-I inhibitor activities of the coastal ethnomedicinal plant terong pungo (Solanum sp.). Jurnal Mikrobiologi Indonesia 2(2).
Holo H, Nilssen, Ness IF. 1991. Lactococcin A: a new bacteriocin from Lactococcus lactis subsp. cremoris: isolation and characterization of the protein and its gene. Journal of Bacteriology 38:79–87.
Iversen BG, Jacobsen T, Eriksen HM, Bukholm G, Melby KK, Nygård K, Aavitsland P. 2007. An outbreak of Pseudomonas aeruginosa infection caused by contaminated mouth swabs. Clinical Infectious Diseases 44:794–801.
Jena SK, Tayung K. 2013. Endophytic fungal communities associated with two ethno-medicinal plants of Similipal Biosphere Reserve, India and their antimicrobial prospective. JAPS 3(4):S7–S12.
Komiyama K, Tynan JJ, Habbick BF, Duncan DE, Liepert DJ. 1985. Pseudomonas aeruginosa in the oral cavity and sputum of patients with cystic fibrosis. Oral Surg Oral Med Oral Pathol 59(6):590–4.
Kusumaningtyas E, Natasia M, Darmono. 2010. Potensi metabolit kapang endofit rimpang lengkuas merah dalam menghambat pertumbuhan Escherichia coli dan Staphylococcus aureus dengan media fermentasi PDB dan PDY. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. 20 Mei 2010. Bogor, Indonesia. p. 819–24.
Lachenmeier DW, Gumbel-Mako S, Sohnius EM, Keck-Wilhelm A, Kratz E, Mildau G. 2009. Salivary acetaldehyde increase due to alcohol-containing mouthwash use: a risk factor for oral cancer. International Journal of Cancer 125:730–5.
Marcotte H, Lavoie MC. 1998. Oral microbial ecology and the role of salivary immunoglobulin A. Microbiology and Molecular Biology Reviews 62(1):71–109.
McCullough MJ, Farah CS. 2008. The role of alcohol in oral carcinogenesis with particular reference to alcohol-containing mouthwashes. Australian Dental Journal 53:302–5.
Meletiadis J, Meis FGM, Mouton JW, Vermeij PE. 2001. Analysis of growth characteristics of filamentous fungi in different nutrient media. Journal of Clinical Microbiology 39(2):478–84.
Millar MR, Walsh TR, Linton CJ. 2001. Carriage of antibiotic-resistant bacteria by healthy children. Journal of Antimicrobial Chemotherapy 47:605–10.
More G, Tshikalange TE, Lall N, Botha F, Meyer JJM. 2008. Antimicrobial activity of medicinal plants against oral microorganisms. Journal of Ethnopharmacology 119(3):473–7.
Sheeba E. 2010. Antibacterial activity of Solanum surattense Burm. F. Kathmandu University Journal of Science, Engineering and Technology 6(1):1–4.
Shulman ST, Phair JP, Somers HM. 1992. The Biological and Clinical Basis of Infectious Disease. 5th Edition. Philadelphia (US): WB Saunders Company. p. 110–6.
Simanjuntak P, Melliawati R, Soekmanto A, Parwati T, Bustanussalam. 2002. Pengembangan bahan baku zat bioaktif antimalaria dari kapang endofit tumbuhan obat Indonesia. Laporan Teknik Penelitian Puslit Biotek-LIPI, Cibinong, Bogor 8(1):49–54.
Srikandace Y, Hapsari Y, Simanjuntak P. 2007. Seleksi mikroba endofit dalam Curcuma zedoaria memproduksi senyawa kimia antimikroba. JIFI 5(2):77–84.
Strobel G, Daisy B, Castillo U, Harper J. 2004. Natural products from endophytic microorganisms. Journal of Natural Products 67:257–68.
Sudantha IM, Abadi AL. 2007. Identifikasi kapang endofit dan mekanisme antagonismenya terhadap Fusarium oxysporum f.sp. vanillae pada tumbuhan vanili. Jurnal Agroteksos 17(1):23–38.
Tarman K. 2011. Biological and Chemical Investigations of Indonesian Marine-Derived Fungi and Their Secondary Metabolites. Göttingen (DE): Cuvillier Verlag. p. 32–5.
Torabinejad M, Walton RE. 2009. Principles and Practice of Endodontics. 4th Edition. Philadelphia (US): Saunders Company. p. 58–63.
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