SELECTION AND CHARACTERIZATION OF SIDEROPHORE-PRODUCING RHIZOBACTERIA AND POTENTIAL ANTAGONISTIC ACTIVITY TOWARD Ralstonia solanacearum
Downloads
Ralstonia solanacearum is an important pathogen of tomato. An alternative method to control this disease is through the application of biocontrol agents. Plant Growth-Promoting Rhizobacteria (PGPR) can be used as potential biocontrol agents, and PGPR producing siderophores play an important role in disease suppression. This experiment was conducted to select and characterize siderophore-producing rhizobacteria from tomato and to determine their potential as antagonistic agents against R. solanacearum. Candidates of PGPR were isolated from tomato plants grown in West Java Province, Indonesia. The isolates were screened for siderophore production using CAS medium. Among the 29 siderophore-producing isolates that showed a negative hypersensitivity reaction, two isolates exhibited the widest inhibition zones against R. solanacearum. These isolates were CP1C and CP2D, with inhibition zone diameters of up to 3.6 mm and 7.0 mm, respectively. Based on 16S rDNA sequencing, isolate CP1C was identified as Brevundimonas sp., while isolate CP2D was identified as Enterobacter sp. Both isolates did not negatively affect plant height or plant dry weight when compared with the control.
Agrios GN. 2005. Plant pathology. Fifth edition. New York (US): Academic Press. 992 p.
Bhattacharya A. 2010. Siderophore-mediated metal uptake by Pseudomonas fluorescens and its comparison to iron (III) chelation. Cey J Sci (Bio Sci) 39(2):147–55.
Gross M. 1990. Siderophores and fluorescent pigments. In: Klement Z, Rudolph K, Sands DC, editors. Methods in phytobacteriology. Budapest (HU): Akadémiai Kiadó. 568 p.
Hu QP, Xu JG. 2011. A simple double-layered Chrome Azurol S agar (SD-CASA) plate assay to optimize the production of siderophores by a potential biocontrol agent. Afr J Microbiol Res 5(25):4321–7.
Jenifer MRA, Reena A, Aysha OS, Valli S, Nirmala P, Vinothkumar P. 2013. Isolation of siderophore-producing bacteria from rhizosphere soil and their antagonistic activity against selected fungal plant pathogens. Int J Curr Microbiol App Sci 2(1):59–65.
Jeung Y, Kim J, Kang Y. 2007. Genetic diversity and distribution of Korean isolates of Ralstonia solanacearum. Plant Dis 91(10):1277–87.
Klement Z, Rudolph K, Sands DC. 1990. Methods in phytobacteriology. Budapest (HU): Akadémiai Kiadó. 568 p.
Louden BC, Haarmann D, Lynne AM. 2011. Use of blue agar CAS assay for siderophore detection. J Microbiol Biol Educ 12(1):51–3.
Neilands JB. 1995. Siderophore: Structure and function of microbial iron transport compounds. J Biol Chem 270(45):26723–6.
Pal RP, Gokarn K. 2010. Siderophores and pathogenicity of microorganisms. J Biosci Tech 1:127–34.
Rachid D, Ahmed B. 2005. Effect of iron and growth inhibitors on siderophore production by Pseudomonas fluorescens. Afr J Biotechnol 4(7):697–702. Available from: http://www.academicjournals.org/AJB
Saharan BS, Nehra V. 2011. Plant growth-promoting rhizobacteria: A critical review. Life Sciences and Medicine Research 2011: LSMR-21. Available from: http://astonjournals.com/lsmr
[Retrieved 24 October 2011].
Sambrook J, Russell DW. 2001. Molecular cloning: A laboratory manual. Third edition. New York (US): Cold Spring Harbor Laboratory Press. p. 6–62.
Sayyed RZ, Badgujar MD, Sonawane HM, Mhaske MM, Chincholkar SB. 2005. Production of microbial iron chelators (siderophores) by fluorescent pseudomonads. Indian J Biotechnol 4:484–90.
Schaad NW, Jones JB, Chun W. 2001. Laboratory guide for identification of plant pathogenic bacteria. Third edition. St. Paul (US): The American Phytopathological Society. 373 p.
Sridevi M, Kumar KG, Mallaiah KV. 2008. Production of catechol-type siderophores by Rhizobium sp. isolated from stem nodules of Sesbania procumbens (Roxb.) W and A. Res J Microbiol 3(4):282–7.
Tian F, Ding Y, Zhu H, Yao L, Du B. 2009. Genetic diversity of siderophore-producing bacteria of tobacco rhizosphere. Braz J Microbiol 40:276–84.
Wang JF, Lin CH. 2005. Integrated management of tomato bacterial wilt. The World Vegetable Center. Available from: http://www.avrdc.org/pdf/PROD5-management_bacterial_wilt.pdf
[Retrieved 26 September 2011].
Yuliar, Nion YA, Toyota K. 2015. Recent trends in control methods for bacterial wilt diseases caused by Ralstonia solanacearum. Microbes Environ 30(1):1–11.
Copyright (c) 2017 BIOTROPIA - The Southeast Asian Journal of Tropical Biology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Authors who publish with this journal agree with the following terms:
- Authors retain copyright and grant the journal right of first publication, with the work 1 year after publication simultaneously licensed under a Creative Commons attribution-noncommerical-noderivates 4.0 International License that allows others to share, copy and redistribute the work in any medium or format, but only where the use is for non-commercial purposes and an acknowledgement of the work's authorship and initial publication in this journal is mentioned.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
