CHARACTERIZATION OF XYLANASE FROM A XYLANOLYTIC- THERMOPHILIC BACTERIUM ISOLATED FROM GUNUNG PANCAR HOT SPRING, WEST JAVA
No. 17 (2001)
Research Paper
November 16, 2011
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days of enrichment in Modified Thermus Medium (MTM) supplemented with 0.5% oat spelt xylan. 16S-
rRNA sequence analysis indicated that IT-08 resembles Bacillus thermoleovorans, a species of thermophilic
bacteria. When grown on xylan containing media, IT-08 produces a thermoactive xylanase. Xylanase from IT-
08 was active at temperatures between 40 and 100°C, at pH values between 4.0 and 9.0 with optimum values
obtained at 80°C and pH 6.0, respectively. SDS-PAGE and zymogram analysis demonstrated that a crude
xylanase complex of IT-08 comprised two active bands with molecular masses of 78 and 60 kDa.
Keywords: xylanase / xylanolytic-thermophilic bacteria
SUWANTO, A., SURYANTO, D., & MERYANDINI, A. (2011). CHARACTERIZATION OF XYLANASE FROM A XYLANOLYTIC- THERMOPHILIC BACTERIUM ISOLATED FROM GUNUNG PANCAR HOT SPRING, WEST JAVA. BIOTROPIA, (17). https://doi.org/10.11598/btb.2001.0.17.167
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