CHARACTERIZATION OF STREPTOMYCES sp. 45I-3 XYLANASE

Authors

  • Anja Meryandini Department of Biology, Faculty of Mathematics and Natural Science, Bogor Agricultural University,Jl. Raya Darmaga, Bogor, Indonesia
  • Deden Saprudin Department of Chemistry, Faculty of Mathematics and Natural Science, Bogor Agricultural University,Jl. Raya Darmaga, Bogor, Indonesia
  • Prima Agung Prihandono Department of Biology, Faculty of Mathematics and Natural Science, Bogor Agricultural University,Jl. Raya Darmaga, Bogor, Indonesia
  • Alina Akhdiya Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Jl. Tentara Pelajar 3A, Bogor, Indonesia
  • Trio Hendarwin PT Sari Husada tbk, Jl. Kusumanegarano 173, Yogyakarta, Indonesia

DOI:

https://doi.org/10.11598/btb.2007.14.2.16

Abstract

Streptomyces sp 45I-3 producing xylanase was isolated from Kalimantan soil sample. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase has an optimum condition at pH 5 and 50 oC.  Crude xylanase have half-life time of 2 hours 9 minute at 50 oC, whereas purified xylanase has half-life time of 12 hours. The molecular mass of purified xylanase was determined to be 43.2 and 39.2 kDa.

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Published

2008-09-02

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Section

Research Paper