AN SNP MARKER POTENTIALLY LINKED TO SOMATIC EMBRYOGENESIS OF OIL PALM (Elaeis guineensis)
DOI:
https://doi.org/10.11598/btb.2017.24.2.678Keywords:
Callogenesis, Elaeis guineensis, SNP, somatic embryogenesisAbstract
Oil palm (Elaeis guineensis) is one of the most important oil-bearing crop in the world. This crop can be vegetatively propagated only using tissue culture technique. Oil palm tissue culture technique has low efficiency, with callogenesis and embryogenesis stages as the limiting factors. Genetic factor has a major role in determining the success rate of these two stages. The use of molecular markers which represent the rate of embryogenesis or callogenesis has the potential to improve the efficiency of oil palm tissue culture process. In this study, SNP mining was conducted on embryogenesis transcriptome data, oil palm cDNA database, oil palm genome database, and oil palm SNPs marker database in NCBI.  The objective of this study was  to obtain SNP marker which represents the embryogenesis potential, to be further used in marker assisted selection of oil palm ortets. One SNP (EMB6) showed significant association with embryogenesis rate. This SNP was found in one of Auxin Response Factor (ARF) family gene. Nucleotide replacement from Adenine to Guanine changed the 307th amino acid from Isoleucine to Methionine. Oil palms with Adenine homozygote (A/A) pattern on the EMB6 showed 8-fold higher chance to produce significantly higher embryogenesis rate than Adenine-Guanine heterozygote (A/G).Downloads
Download data is not yet available.
Downloads
Published
2017-08-31
How to Cite
Wahyunanto, I., Waturangi, D. E., Toruan-Mathius, N., & Yulandi, A. (2017). AN SNP MARKER POTENTIALLY LINKED TO SOMATIC EMBRYOGENESIS OF OIL PALM (Elaeis guineensis). BIOTROPIA - The Southeast Asian Journal of Tropical Biology, 24(2), 153–160. https://doi.org/10.11598/btb.2017.24.2.678
Issue
Section
Research Paper
License
Authors who publish with this journal agree with the following terms:
- Authors retain copyright and grant the journal right of first publication, with the work 1 year after publication simultaneously licensed under a Creative Commons attribution-noncommerical-noderivates 4.0 International License that allows others to share, copy and redistribute the work in any medium or format, but only where the use is for non-commercial purposes and an acknowledgement of the work's authorship and initial publication in this journal is mentioned.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).