RAPD Analysis to Detect Somaclonal Variation of Pineapple In Vitro Cultures during Micropropagation
AbstractPlant off-types still become a concern in pineapple micropropagation. Reliable methods are needed to detect and to reduce plant off-types. This research was conducted to confirm the occurrence of somaclonal variation during micropropagation, to know the level of variation in three different populations, to detect and to reduce the somaclonal variation of pineapple clone Simadu. The first population was the acclimatized plants and plantlets of four-year old cultures. The second population was the plantlets yielded from three kinds of regeneration methods (direct organogenesis, indirect organogenesis, somatic embryogenesis) derived from the normal phenotype shoots selected from the first population. The third population was the new in vitro cultures regenerated by shoot proliferation and direct organogenesis methods. RAPD analysis by using 10 primers was performed to confirm genetic variation. The result showed that the phenotypic variation occurred in the four year-old pineapple plants and plantlets were due to genetic variation. This research has proved that the long-period of cultures is the main contributor of somaclonal variation, while the regeneration method and plant growth regulator could also induce genetic variation. The new cultures showed higher level of similarity. Therefore, it needs a correct strategy to apply micropropagation method.
LicenseAuthors who publish with this journal agree with the following terms:
- Authors retain copyright and grant the journal right of first publication, with the work 1 year after publication simultaneously licensed under a Creative Commons attribution-noncommerical-noderivatesÂ 4.0 InternationalÂ License that allows others to share, copy and redistribute the work in any medium or format,Â but only where the use is for non-commercial purposesÂ and an acknowledgement of the work's authorship and initial publication in this journal is mentioned.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).