ADDITION OF ESSENTIAL OIL SOURCE, Amomum compactum Soland. ex Maton, AND ITS EFFECT ON RUMINAL FEED FERMENTATION IN-VITRO
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Essential oil (EO), as a feed additive, is known to increase feed efficiency and reduce methane production in ruminants. This research was conducted to study the effect of Java cardamom (Amomum compactum Soland ex Maton) essential oil as a feed additive on ruminal feed fermentation. The in vitro gas production technique was used in this research to determine the effect of cardamom on nutrient digestibility or fermentation in the rumen. Cardamom meal was added into the feed sample to obtain final EO concentrations in the fermentation medium of 0, 25, 50, 75, and 100 mg/L. The substrate consisted of Pennisetum purpureum, rice bran, and wheat pollard. The addition of cardamom did not significantly affect dry matter digestibility, except at 100 mg/L, where it decreased. Protein digestibility decreased when the diet was supplemented with cardamom, whereas organic matter and crude fiber digestibility increased by up to 13.5% and 24% at the 100 mg/L EO level, respectively. The production of volatile fatty acids (acetate, propionate, butyrate), pH, and microbial protein synthesis, except ammonia concentration, were not affected by cardamom addition. Similarly, methane production and protozoa population did not significantly change. The utilization of Java cardamom as a feed additive positively affected ruminal fermentation by increasing organic matter and crude fiber digestibility, while reducing protein digestibility.
Abreu A, Camila JE, Lascano CE, Diaz TE, Kreuzer M, Hess HD. 2004. Effects of Sapindus saponaria fruits on ruminal fermentation and duodenal nitrogen flow of sheep fed a tropical grass diet with and without legume. J Anim Sci 82:1392–400.
Alexander RR, Griffith JM. 1993. Basic Biochemical Methods. 2nd ed. New York (US): Wiley-Liss, Inc.
Araujo RC, Pires AV, Mourio GB, Abdalla AL, Sallam SMA. 2011. Use of blanks to determine in vitro net gas and methane production when using rumen fermentation modifiers. Anim Feed Sci Technol 166-167:155–62.
Bakkali F, Averbeck S, Averbeck D, Idaomar M. 2008. Biological effects of essential oils: A review. Food Chem Toxicol 46:446–75.
Belanche A, De Fuente G, Newbold CJ. 2014. Study of methanogen communities associated with different rumen protozoal populations. FEMS Microbiol Ecol 90:663–77.
Benchaar C, Greathead H. 2011. Essential oils and opportunities to mitigate enteric methane emissions from ruminants. Anim Feed Sci Technol 166-167:338–55.
Bodas R, Prieto N, Garcia-González R, Andrés S, Giráldez FJ, López S. 2012. Manipulation of rumen fermentation and methane production with plant secondary metabolites. Anim Feed Sci Technol 176:78–93.
Busquet M, Calsamiglia S, Ferret A, Kamel C. 2006. Plant extracts affect in vitro rumen microbial fermentation. J Dairy Sci 89:761–71.
Calsamiglia S, Busquet M, Cardozo PW, Castillejos L, Ferret A. 2007. Invited review: Essential oils as modifiers of rumen microbial fermentation. J Dairy Sci 90:2580–95.
Cardozo PW, Calsamiglia S, Ferret A, Kamel C. 2006. Effects of alfalfa extract, anise, capsicum, and a mixture of cinnamaldehyde and eugenol on ruminal fermentation and protein degradation in beef heifers fed a high-concentrate diet. J Anim Sci 84:2801–8.
Castillejos L, Calsamiglia S, Ferret A, Losa R. 2005. Effects of a specific blend of essential oil compounds and the type of diet on rumen microbial fermentation and nutrient flow from a continuous culture system. Anim Feed Sci Technol 119:29–41.
Castillejos L, Calsamiglia S, Ferret A. 2006. Effect of essential oil active compounds on rumen microbial fermentation and nutrient flow in in vitro systems. J Dairy Sci 89:2649–58.
Chaney AL, Marbach EP. 1962. Modified reagents for determination of urea and ammonia. Clin Chem 8:130–2.
Chao SC, Young DG, Oberg CJ. 2012. Screening for inhibitory activity of essential oils on selected bacteria, fungi and viruses. J Essent Oil Res 12:37–41.
Chaudhry AS, Mehedi M, Khan H. 2012. Impacts of different spices on in vitro rumen dry matter disappearance, fermentation, and methane of wheat or ryegrass hay-based substrates. Livest Sci 146:84–90.
Chempakam B, Sindhu S. 2008. Large cardamom. In: Parthasarathy VA, Chempakam B, Zachariah T, editors. Chemistry of Spices. London (UK): CAB International. p. 59–69.
Choudhury PK, Salem AZM, Jena R, Kumar S, Singh AKR. 2015. Rumen microbiology: An overview. In: Puniya AK, Singh R, Kamra DN, editors. Rumen Microbiology: From Evolution to Revolution. India: Springer India.
Cobellis G, Trabalza-Marinucci M, Yu Z. 2016a. Evaluation of different essential oils in modulating methane and ammonia production, rumen fermentation, and rumen bacteria in vitro. Anim Feed Sci Technol 215:25–36.
Cobellis G, Trabalza-Marinucci M, Yu Z. 2016b. Critical evaluation of essential oils as rumen modifiers in ruminant nutrition: A review. Sci Total Environ 545–546:556–68.
Compiani R, Sgoifo Rossi CA, Pizzi A, Dell’Orto V. 2013. Administration of essential oils to beef cattle: Effects on health status and growth performance. In: Boiti C, Ferlazzo A, Gaiti A, Pugliese A, editors. Trends in Veterinary Sciences. Berlin (DE): Springer. p. 117–180.
Cosentino S, Tuberoso C, Pisano B, Satta M, Mascia V, Arzedi E, Palmas F. 1999. In vitro antimicrobial activity and chemical composition of Sardinian flowers essential oils. Lett Appl Microbiol 29:130–5.
Deans SG, Ritchie G. 1987. Antibacterial properties of plant essential oils. Int J Food Microbiol 5:165–80.
Eckard RJ, Grainger C, de Klein CAM. 2010. Options for the abatement of methane and nitrous oxide from ruminant production: A review. Livest Sci 130:47–56.
Ferme D, Banjac M, Calsamiglia S, Busquet M, Kamel C, Avgustin G. 2004. Effects of extracts on microbial community structure in a rumen-simulating continuous culture system. Folia Microbiol 49:151–5.
Fraser GRS, Chaves AV, Wang Y, McAllister TA, Beauchemin KA, Benchaar C. 2007. Assessment of cinnamon leaf oil on rumen fermentation using continuous culture systems. J Dairy Sci 90:2315–28.
Geraci JI, Garciarena AD, Gagliostro GA, Beauchemin KA, Colombatto D. 2012. Effects of cinnamaldehyde, eugenol and capsicum oleoresin supplementation on rumen environment and animal performance. Anim Feed Sci Technol 176:123–30.
Jouany JP, Morgavi DP. 2007. Use of natural products as alternatives to antibiotic feed additives in ruminant production. Animal 1:1443–66.
Kalemba D, Kunicka A. 2003. Antibacterial and antifungal properties of essential oils. Curr Med Chem 10:813–29.
Kalemba D, Matla M, Smetek A. 2012. Antimicrobial activities of essential oils. In: Patra AK, editor. Dietary Phytochemicals and Microbes. New York (US): Springer. p.153–84.
Khorrami B, Vakili AR, Mesgaran MD, Klevenhusen F. 2015. Thyme and cinnamon essential oils as alternatives to monensin in beef cattle. Anim Feed Sci Technol 200:8–16.
Kotzekidou P, Giannakidis P, Boulamatsis A. 2008. Antimicrobial activity of plant extracts and essential oils against foodborne pathogens. Food Sci Technol 41:119–27.
Kumar S, Puniya AK, Puniya M, Dagar SS, Sirohi SK, Singh K, Griffith GW. 2009. Factors affecting rumen methanogens and methane mitigation strategies. World J Microbiol Biotechnol 25:1557–66.
Macheboeuf D, Morgavi DP, Papon Y, Mousset JL, Arturo-Schaan M. 2008. Dose-response effects of essential oils on rumen microbial fermentation. Anim Feed Sci Technol 145:335–50.
McIntosh FM, Williams P, Losa R, Wallace RJ, Newbold CJ, Beever DA. 2003. Effects of essential oils on ruminal microorganisms and protein metabolism. Appl Environ Microbiol 69:5011–4.
Moran J. 2005. Tropical Dairy Farming: Feeding Management for Smallholder Dairy Farmers in the Humid Tropics. England (UK): Landlinks Press. p.41–49.
Morgavi DP, Forano E, Martin C, Newbold CJ. 2010. Microbial ecosystem and methanogenesis in ruminants. Animal 4:1024–36.
Nagaraja TG. 2016. Microbiology of the rumen. In: Millen D, Arrigoni MB, Pacheco RDL, editors. Rumenology. Cham (CH): Springer. p. 39–61.
Newbold CJ, McIntosh FM, Williams P, Losa R, Wallace RJ. 2004. Effects of a specific blend of essential oils on rumen fermentation. Anim Feed Sci Technol 114:105–12.
Patra AK. 2011. Effects of essential oils on rumen fermentation, microbial ecology and ruminant production. Asian J Anim Vet Adv 6:416–28.
Patra AK, Saxena J. 2009. Dietary phytochemicals as rumen modifiers: Effects on microbial populations. Antonie van Leeuwenhoek Int J Gen Mol Microbiol 96:363–75.
Patra AK, Yu Z. 2012. Effects of essential oils on methane production and rumen microbial communities. Appl Environ Microbiol 78:4271–80.
Russell JB, Strobel HJ. 1989. Effect of ionophores on ruminal fermentation. Appl Environ Microbiol 55:1–6.
Salles MSV, Zanetti MA, Titto EAL, Conti RMC. 2008. Effect of monensin on performance of growing ruminants under different environmental temperatures. Anim Feed Sci Technol 147:279–91.
Sardar BR, Tarade KM, Singhal RS. 2013. Stability of cardamom oleoresin in co-crystallized sugar cubes during storage. J Food Eng 117:530–7.
Sivropoulou A, Papanikolaou E, Nikolaou C, Kokkini S, Lanaras T, Arsenakis M. 1996. Antimicrobial and cytotoxic activities of oregano essential oils. J Agric Food Chem 44:1202–5.
Spanghero M, Zanfi C, Fabbro E, Scicutella N, Camellini C. 2008. Effects of a blend of essential oils on rumen fermentation. Anim Feed Sci Technol 145:364–74.
Theodorou MK, Williams BA, Dhanoa MS, McAllan AB, France J. 1994. A simple gas production method using a pressure transducer to determine fermentation kinetics. Anim Feed Sci Technol 48:185–97.
U.S. Food & Drug Administration. 2017. Generally recognized as safe (GRAS). Silver Spring (MD): FDA. Available from: https://www.fda.gov/food/food-ingredients-packaging/generally-recognized-safe-gras
Wallace RJ, McEwan NR, McIntosh FM, Teferedegne B, Newbold CJ. 2002. Natural manipulators for rumen fermentation. Asian-Aust J Anim Sci 15:1458–68.
Wanapat M, Kongmun P, Poungchompu O, Cherdthong A, Khejornsart P, Pilajun R, Kaenpakdee S. 2012. Effects of plants containing secondary compounds and plant oils on rumen fermentation and ecology. Trop Anim Health Prod 44:399–405.
Wanapat M, Kang S, Khejornsart P, Wanapat S. 2013. Effects of plant herb combinations on rumen fermentation and nutrient digestibility in beef cattle. Asian-Aust J Anim Sci 26:1127–36.
Wang SP, Wang WJ. 2016. Effects of dietary supplementation of Chinese herbal mixtures on rumen fermentation and nutrient digestion in goats. S Afr J Anim Sci 46:247–60.
Wang Y, McAllister TA. 2002. Rumen microbes, enzymes and digestion: A review. Asian-Aust J Anim Sci 15:1659–76.
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