ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND PHENOLIC- AND CATECHOL-DEGRADING Enterobacter sp. STRAIN SAW-2

Mohd Khalizan Sabullah, Mohd Fadhil Rahman, Siti Aqlima Ahmad, Mohd Rosni Sulaiman, Mohd Shukri Shukor, Azlan Jualang Gansau, Nor Aripin Shamaan, Mohd Yunus Shukor

Abstract


Molybdenum is an emerging pollutant worldwide. The objective of this study is to isolate molybdenum-reducing bacterium with the ability to grow on phenolic compounds (phenol and catechol). The screening process was carried out on a microplate. The bacterium reduced molybdenum in the form of sodium molybdate to molybdenum blue (Mo-blue). The bacterium required a narrow pH range for optimal reduction of molybdenum, i.e. between  pH 6.3 and 6.8, with temperature between 34 and 37 oC. Molybdate reduction to Mo-blue was best supported by glucose as the carbon source. However, both phenol and catechol could not support molybdate reduction. Other requirements for molybdate reduction included sodium molybdate concentrations between 15 and 30 mM, and phosphate concentration of 5.0 mM. The bacterium exhibited a Mo-blue absorption spectrum with a shoulder at 700 nm and a maximum peak near the infrared region at 865 nm. The Mo-reducing bacterium was partially identified as Enterobacter sp. strain Saw-2. The capability of this bacterium to grow on toxic phenolic compounds and to detoxify molybdenum made it a significant agent for bioremediation.


Keywords


Catechol, Enterobacter sp., Molybdenum, molybdenum blue, phenol

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DOI: http://dx.doi.org/10.11598/btb.2017.24.1.550

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