V. PRELIMINARY STUDY ON ISOZYMES OF SHOREA JAVANICA
AbstractThe detection of genetic variability in natural or man-made populations/ plantations is useful in both basic and applied biology. In addition to the various facets of studies on ShoreaÂ javanicaÂ alreadyÂ initiatedÂ byÂ TorquebiauÂ (1984)Â andÂ alongsideÂ withÂ his recommendationsÂ onÂ focusÂ forÂ futureÂ research,Â aÂ studyÂ onÂ theÂ geneticÂ aspectsÂ ofÂ the species should be givenÂ importantÂ considerations. AsÂ theÂ trees areÂ tappedÂ forÂ resin, an importantÂ forestÂ product,Â theÂ geneticÂ basisÂ ofÂ theÂ productionÂ as wellÂ asÂ theÂ rangeÂ of variation in amount of resin production amongÂ the trees must be known. Coupled with this is a thorough investigation on the differences in pest resistance/susceptability among theÂ trees andÂ their genetic basis. WhileÂ the assumptionÂ (Torquebiau 1984)Â thatÂ treesÂ in naturalÂ forestÂ areasÂ are-rarelyÂ attackedÂ byÂ diseasesÂ becauseÂ ofÂ mycorrhizalÂ fungiÂ is interesting, its confirmation is necessary. If this is true, problems would arise when plants are introduced into a new plantation site as experienced by the Forest Research Institute (Ardikoesuma 1954). Thus, we needÂ toÂ lookÂ for pestÂ resistant plantsÂ i.e.Â thoseÂ that can remain healthy even in the absence of mycorrhizae. TheÂ aboveÂ studiesÂ onÂ possible geneticÂ variationÂ couldÂ giveÂ vitalÂ informationÂ for development of forest plantations of the species and for breeding and tree improvement strategies.Â ByÂ knowingÂ theÂ extentÂ ofÂ geneticÂ variationÂ inÂ naturalÂ populationÂ orÂ in plantations one could be guided to maintain or increase the genetic base in these areas. Biochemical characters such as isozyme banding patterns have been useful in several areasÂ ofÂ plantÂ biology,Â populationÂ genetics,Â evolutionÂ andÂ breeding.Â IsozymesÂ are detected by starch gel electrophoresis and when their genetic controlÂ is established, they couldÂ beÂ geneticÂ markersÂ inÂ analyzingÂ variationÂ inÂ morphologicalÂ orÂ physiological characters. TheÂ presentÂ studyÂ isÂ anÂ attemptÂ toÂ detectÂ theÂ isozymesÂ inÂ leaves,Â seedsÂ and cotyledons of Shorea javanica by gel electrophoresis.
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