ISOLATING MICROSATELLITE FROM Amorphophallus variabilis AND ITS APPLICATION FOR POPULATION STUDY IN DRAMAGA CONSERVATION FOREST, INDONESIA

Araceae genetic population SSR tuber crop white iles-iles

Authors

  • Edi Santosa
    editodai@yahoo.com
    Faculty of Agriculture, Bogor Agricultural University, Indonesia http://orcid.org/0000-0001-5560-4598
  • Chun Lan Lian Asian Natural Environmental Science Center, The University of Tokyo, Midori-cho 1-1-8 Nishitokyoshi, Tokyo 188-0002, Japan, Japan
  • Yoko Mine Faculty of Agriculture, Tokyo University of Agriculture, Atsugi, Kanagawa 243-0034, Japan, Japan
  • Ken Takahata Faculty of Agriculture, Tokyo University of Agriculture, Atsugi, Kanagawa 243-0034, Japan, Japan
  • Nobuo Sugiyama Faculty of Agriculture, Tokyo University of Agriculture, Atsugi, Kanagawa 243-0034, Japan, Japan
Vol. 25 No. 1 (2018)
Research Paper
July 17, 2017
April 26, 2018

Downloads

Amorphophallus variabilis Blume, a member of Araceae, is a fleshy perennial tuber crop endemic in Java Island, Indonesia. The plant produces white edible corm; and it was used as food during famine time before 1960s. Rapid ecological changes and land fragmentations in Java in recent times threaten populations of A. variabilis. Here, compound microsatellite markers were developed in order to develop conservation strategies in the populations. Twelve primers pairs produced high polymorphism ranging from 5 to 22 alleles per locus. The observed and expected heterozygosities ranged from 0.191 to 0.851 and 0.380 to 0.943, respectively. This high allelic diversity indicates that these markers are suitable for the study on population genetic structure. Cross-amplification on related and non-related species was performed. Application of the markers on populations from Dramaga Conservation Forest revealed high allelic richness, high diversity within and among populations. Genetic distance among populations increased with an increase of geographic distance. Present study suggested that, it is important to study population of A. variabilis in Java in order to understand the population genetic structure and develop effective in situ conservation programs.