ISOLATING MICROSATELLITE FROM Amorphophallus variabilis AND ITS APPLICATION FOR POPULATION STUDY IN DRAMAGA CONSERVATION FOREST, INDONESIA

Authors

  • Edi Santosa Faculty of Agriculture, Bogor Agricultural University http://orcid.org/0000-0001-5560-4598
  • Chun Lan Lian Asian Natural Environmental Science Center, The University of Tokyo, Midori-cho 1-1-8 Nishitokyoshi, Tokyo 188-0002, Japan
  • Yoko Mine Faculty of Agriculture, Tokyo University of Agriculture, Atsugi, Kanagawa 243-0034, Japan
  • Ken Takahata Faculty of Agriculture, Tokyo University of Agriculture, Atsugi, Kanagawa 243-0034, Japan
  • Nobuo Sugiyama Faculty of Agriculture, Tokyo University of Agriculture, Atsugi, Kanagawa 243-0034, Japan

DOI:

https://doi.org/10.11598/btb.2018.25.1.652

Keywords:

Araceae, genetic population, SSR, tuber crop, white iles-iles

Abstract

Amorphophallus variabilis Blume, a member of Araceae, is a fleshy perennial tuber crop endemic in Java Island, Indonesia. The plant produces white edible corm; and it was used as food during famine time before 1960s. Rapid ecological changes and land fragmentations in Java in recent times threaten populations of A. variabilis. Here, compound microsatellite markers were developed in order to develop conservation strategies in the populations. Twelve primers pairs produced high polymorphism ranging from 5 to 22 alleles per locus. The observed and expected heterozygosities ranged from 0.191 to 0.851 and 0.380 to 0.943, respectively. This high allelic diversity indicates that these markers are suitable for the study on population genetic structure. Cross-amplification on related and non-related species was performed. Application of the markers on populations from Dramaga Conservation Forest revealed high allelic richness, high diversity within and among populations. Genetic distance among populations increased with an increase of geographic distance. Present study suggested that, it is important to study population of A. variabilis in Java in order to understand the population genetic structure and develop effective in situ conservation programs.

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Author Biographies

Edi Santosa, Faculty of Agriculture, Bogor Agricultural University

Associate Professor, Dept Agronomy and Horticulture

Chun Lan Lian, Asian Natural Environmental Science Center, The University of Tokyo, Midori-cho 1-1-8 Nishitokyoshi, Tokyo 188-0002, Japan

Associate Professor

Yoko Mine, Faculty of Agriculture, Tokyo University of Agriculture, Atsugi, Kanagawa 243-0034, Japan

Dr

Ken Takahata, Faculty of Agriculture, Tokyo University of Agriculture, Atsugi, Kanagawa 243-0034, Japan

Dr

Nobuo Sugiyama, Faculty of Agriculture, Tokyo University of Agriculture, Atsugi, Kanagawa 243-0034, Japan

Professor

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Published

2018-04-26

How to Cite

Santosa, E., Lian, C. L., Mine, Y., Takahata, K., & Sugiyama, N. (2018). ISOLATING MICROSATELLITE FROM Amorphophallus variabilis AND ITS APPLICATION FOR POPULATION STUDY IN DRAMAGA CONSERVATION FOREST, INDONESIA. BIOTROPIA - The Southeast Asian Journal of Tropical Biology, 25(1), 22–32. https://doi.org/10.11598/btb.2018.25.1.652

Issue

Vol. 25 No. 1 (2018)

Section

Research Paper

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