VIABILITY AND PLASMA MEMBRANE INTEGRITY OF THE SPOTTED BUFFALO EPIDIDYMAL SPERMATOZOA AFTER THAWING WITH THE ADDITION OF DEXTROSE INTO THE EXTENDER
Downloads
The objective of this study was to obtain the viability and plasma membrane integrity of the spotted buffalo epididymal sperm after addition of dextrose into AndromedÒ extender. Spermatozoa that have been collected from cauda epididymis were diluted with AndromedÒ extender as control (K) and AndromedÒ + 0.2% dextrose (P1) and AndromedÒ + 0.4% dextrose (P2) as treatments. The results showed that the quality of epididymal spermatozoa decreased during cryopreservation process. The percentage of motility after thawing in P1 (46%) and P2 (46.67%) were significantly higher (P<0.05) compared to K (41%) as well as the percentage of live sperm in P1 (58.8%) and P2 (60%) compared to K (52.2%). The percentage of membrane integrity in P1, P2 and K were 67.4; 66.8 and 68 %, respectively. In conclusion, the addition of 0.2 and 0.4% of dextrose into AndromedÒ acted as an extra cellular cryoprotectant and could maintain the viability and membrane integrity of the spotted buffalo epididymal spermatozoa after thawing.
Â
Key words: epididymal sperm, cryopreservation, dextrose, spotted buffalo
Downloads
Copyright (c) 2017 BIOTROPIA - The Southeast Asian Journal of Tropical Biology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Authors who publish with this journal agree with the following terms:
- Authors retain copyright and grant the journal right of first publication, with the work 1 year after publication simultaneously licensed under a Creative Commons attribution-noncommerical-noderivates 4.0 International License that allows others to share, copy and redistribute the work in any medium or format, but only where the use is for non-commercial purposes and an acknowledgement of the work's authorship and initial publication in this journal is mentioned.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
