DEVELOPMENT OF ANTIBODY TO RALSTONIA SOLANACEARUM AND ITS APPLICATION FOR DETECTION OF BACTERIAL WILT
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The serological assay for the detection of bacterial wilt caused by Ralstonia solanacearum RS
was able to provide information regarding the presence of the pathogen in plant materials The
research is was aimed to develop polyclonal antibody PAb for RS detection Bacterial whole
cells of RS isolates mixed with glutaraldehyde were used to immunize New Zealand female
white rabbit The titre of antibody in culture supernatant was 1 1024 The PAb developed from
a ground nut RS isolates reacted with infected plant samples from various locations It was able
to detect RS antigen of crude extract and pure cultures from tomato and potato plant samples
45 using dot blot ELISA however the minimum detectable concentration of RS antigen was 10
cellsml The PAb obtained in this study is sensitive enough to detect RS isolates in routine
serological assay
Key words Bacterial wilt Ralstonia solanacearum antibody dot blot ELISA
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