DEVELOPMENT OF ANTIBODY TO RALSTONIA SOLANACEARUM AND ITS APPLICATION FOR DETECTION OF BACTERIAL WILT
AbstractThe serological assay forÂ the detection ofÂ bacterial wilt caused by RalstoniaÂ solanacearumÂ (RS) was able to provideÂ information regarding the presence ofÂ the pathogenÂ in plant materials. The research is was aimed to develop polyclonal antibody (PAb) for RS detection. Bacterial whole cells ofÂ RS isolates mixed with glutaraldehyde were used to immunize New Zealand female whiteÂ rabbit. TheÂ titre ofÂ antibodyÂ in culture supernatant was 1: 1024. The PAb developedÂ from a ground nut RSÂ isolates reacted withÂ infected plant samples from variousÂ locations. It was able to detect RS antigen ofÂ crude extract and pure cultures from tomato and potato plant samples 4-5 using dot blot ELISA; however, the minimum detectable concentration ofÂ RS antigen was 10cells/ml. The PAb obtained in this study is sensitive enough to detect RS isolates in routine serological assay.Key words:Â Bacterial wilt, RalstoniaÂ solanacearum, antibody, dot blot ELISA.
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