DEVELOPMENT OF ANTIBODY TO RALSTONIA SOLANACEARUM AND ITS APPLICATION FOR DETECTION OF BACTERIAL WILT

Authors

  • YADI SURYADI
    yshid@yahoo.co.uk
    Bacteriology Laboratory, Department. of Biochemistry, ICABIOGRAD, Indonesia
Vol. 16 No. 2 (2009)
Research Paper
July 29, 2011
July 14, 2011

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The serological assay for  the detection of  bacterial wilt caused by Ralstonia  solanacearum  RS
was able to provide  information regarding the presence of  the pathogen  in plant materials The
research is was aimed to develop polyclonal antibody PAb for RS detection Bacterial whole
cells of  RS isolates mixed with glutaraldehyde were used to immunize New Zealand female
white  rabbit The  titre of  antibody  in culture supernatant was 1 1024 The PAb developed  from
a ground nut RS  isolates reacted with  infected plant samples from various  locations It was able
to detect RS antigen of  crude extract and pure cultures from tomato and potato plant samples
45 using dot blot ELISA however the minimum detectable concentration of  RS antigen was 10
cellsml The PAb obtained in this study is sensitive enough to detect RS isolates in routine
serological assay

Key words  Bacterial wilt Ralstonia  solanacearum antibody dot blot ELISA