CELLULASE PRODUCTION BY Bacillus subtilis M1 USING PRETREATED GROUNDNUT SHELL BASED LIQUID STATE FERMENTATION

Authors

  • Ashish Kr Vyas LPU PHAGWARA
  • Chayanika Putatunda
  • Joginder Singh
  • Deepak Vyas

DOI:

https://doi.org/10.11598/btb.2016.23.1.472

Abstract

Groundnut shell which is rich in natural cellulose was assessed as a substrate for production of cellulase enzyme by cellulolytic bacteria. In the present investigation the bacterial isolate Bacillus subtilis M1 was found to be capable of producing high amount of endoglucanase and exoglucanase on alkali treated groundnut shell. The effect of some nitrogen sources, amino acids and Ca++ ionsin the medium containing pretreated groundnut shell were also evaluated. It was observed that 2% substrate concentration, 1mM calcium concentration were optimum for cellulase production. Ammonium nitrate was found to be the best among nitrogen sources tested. Asparagine, tryptophan and methionine were found to be stimulatory for cellulase activity.

Keywords: Bacillus subtilis, cellulase, endoglucanase, exoglucanase, groundnut shell

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Author Biography

Ashish Kr Vyas, LPU PHAGWARA

DR ASHISH VYAS IS PRESENTLY WORKING AS ASSISTANT PROFESSOR AND HOD DOMAIN MICROBIOLOGY ,SCHOOL OF BIOSCIENCES AND BIOTECHNOLOGYLOVELY PROFESSIONAL UNIVERSITY, PHAGWARA.   

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Published

2016-08-03

How to Cite

Vyas, A. K., Putatunda, C., Singh, J., & Vyas, D. (2016). CELLULASE PRODUCTION BY Bacillus subtilis M1 USING PRETREATED GROUNDNUT SHELL BASED LIQUID STATE FERMENTATION. BIOTROPIA - The Southeast Asian Journal of Tropical Biology, 23(1), 28–34. https://doi.org/10.11598/btb.2016.23.1.472

Issue

Section

Research Paper