Elimination Of CVB (Chrysanthemum Virus B) From A Range Of ChrysanthemumTHE Varieties By Meristem Culture Following Antiviral and Heat Treatments
AbstractCVB elimination for retaining healthy protocols from infected chrysanthemum plant was investigated trough combined treatment of meristem culture with synthetic antiviral ribavirin and thermotherapy under in vitro conditions. The biological material used on the experimentation was constituted of six commercial varieties: Dewi Sartika, Saraswati, Yellow Fiji, White Puma, Yellow Puma and White Reagent.Â Tissue culture initiation was conducted through plantlet establishment using MS supplemented with IAA.Â Ribavirin was added in media treatments with the concentration of 40 mg/l.Â Parallel with this step, heat treatment with different durations (1, 2, and 3 weeks) was also conducted on the plantlets.Â Meristem culture was done following the chemo- and thermotherapy.Â The experiment resumed the failure single treatment of meristem culture in eliminating CVB from the infected chrysanthemum plantlets.Â Under heat treatment, percentage of virus-free plantlets increased along with the duration of thermotherapy, though the survival rate of plantlets decreased in lengthened heat treatment. The best results regarding virus free plant percentage was obtained when meristem culture was applied following ribavirin or three weeks heat treatment which effectively eliminated virus particles from the plant.
Download data is not yet available.
How to Cite
Budiarto, K., Budi, B. M., Lia, L. S., Muchdar, M. S., & Indijarto, I. B. R. (2012). Elimination Of CVB (Chrysanthemum Virus B) From A Range Of ChrysanthemumTHE Varieties By Meristem Culture Following Antiviral and Heat Treatments. BIOTROPIA - The Southeast Asian Journal of Tropical Biology, 18(2). https://doi.org/10.11598/btb.2011.18.2.45
LicenseAuthors who publish with this journal agree with the following terms:
- Authors retain copyright and grant the journal right of first publication, with the work 1 year after publication simultaneously licensed under a Creative Commons attribution-noncommerical-noderivatesÂ 4.0 InternationalÂ License that allows others to share, copy and redistribute the work in any medium or format,Â but only where the use is for non-commercial purposesÂ and an acknowledgement of the work's authorship and initial publication in this journal is mentioned.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).