BACTERIAL COMMUNITY SHIFTS OF A HIGH MOUNTAIN LAKE IN RESPONSE TO VARIABLE SIMULATED CONDITIONS: AVAILABILITY OF NUTRIENTS, LIGHT AND OXYGEN

Authors

  • MUNTI YUHANA 'Department ofAguamlture, Faculty of Fisheries and Marine Science,
  • THOMAS HORATH Department of Microbiology, Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, 8008 Ziirich, Switzerland
  • KURT HANSELMANN Department of Microbiology, Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, 8008 Ziirich, Switzerland

DOI:

https://doi.org/10.11598/btb.2006.13.2.219

Abstract

We studied bacterial population composition shifts by exposing natural water samples to variable simulated environmental conditions.
The samples were taken from Lake Jori XIII (2640 m a.s.l), an oligo-to mesotrophic cold freshwater lake, located in the eastern Swiss
Alps. The Jori lakes are characterized as remote, unpolluted high mountain lakes with a long period of ice cover and typically low
nutrient concentrations. Culture independent techniques (PCR-based analyses) were used for detection and molecular
characterization of a large number of bacteria most of which are still uncultivable. Bacterial community shifts over three ecological
conditions (nutrients, light and oxygen availability) were detected by using Temporal Temperature gradient Gel Electrophoresis (TTGE)
of a PCR-amplified part of the 16S rRNA gene. The bacterial populations responded differently to the variable conditions, as
revealed by TTGE pattern shifts during the experiment.
Key words: Temporal Temperature gradient Gel Electrophoresis (TTGE), ARB, small subunit ribosomal RNA gene (SSU rRNA gene),
alpine freshwater lake JOri XIII, PCR

Downloads

Download data is not yet available.

Downloads

How to Cite

YUHANA, M., HORATH, T., & HANSELMANN, K. (2011). BACTERIAL COMMUNITY SHIFTS OF A HIGH MOUNTAIN LAKE IN RESPONSE TO VARIABLE SIMULATED CONDITIONS: AVAILABILITY OF NUTRIENTS, LIGHT AND OXYGEN. BIOTROPIA, 13(2). https://doi.org/10.11598/btb.2006.13.2.219

Issue

Vol. 13 No. 2 (2006)

Section

Research Paper

License

Authors who publish with this journal agree with the following terms:

  1. Authors retain copyright and grant the journal right of first publication, with the work 1 year after publication simultaneously licensed under a Creative Commons attribution-noncommerical-noderivates 4.0 International License that allows others to share, copy and redistribute the work in any medium or format, but only where the use is for non-commercial purposes and an acknowledgement of the work's authorship and initial publication in this journal is mentioned.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).