RECENT COLLECTION OF THE POISONOUS MUSHROOM, Clarkeinda trachodes (Berk.) FOR INDONESIA

Agaricales Clarkeinda poisonous taxonomy West Java

Authors

  • Ivan Permana Putra
    ivanpermanaputra@apps.ipb.ac.id
    Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Agatis, Dramaga Campus, Bogor,16680, Indonesia
  • Oktan Dwi Nurhayat Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Bogor 16911, Indonesia, Indonesia
  • Wahyu AJi Mahardika Microbiology of Post Graduate Program, Department of Biology, Faculty of Mathematic and Natural Sciences, IPB University, Bogor 16680, Indonesia, Indonesia
  • Okta Yulia Sari Microbiology of Post Graduate Program, Department of Biology, Faculty of Mathematic and Natural Sciences, IPB University, Bogor 16680, Indonesia, Indonesia
February 21, 2024
April 18, 2024
RECENT COLLECTION OF THE POISONOUS MUSHROOM, Clarkeinda trachodes (Berk.) FOR INDONESIA

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Clarkeinda trachodes is an interesting agaric and has been acknowledged as one of the poisonous mushrooms worldwide. This species has mentioned to be found in tropical regions of Asia, including Indonesia. However, no clear record has been found regarding the primary information and herbarium collection of this species in Indonesia. During our regular mushroom foraging at IPB University campus forest Indonesia, the basidiomata of Clarkeinda was encountered. The current study aimed to confirm the taxonomical position of our specimens based on morphological and molecular evidence. Fresh basidomata was observed on the macro- and micromorphological features. The molecular analysis and phylogenetic tree construction were done based on ITS 1/2 sequence. The combination of morphological and molecular evidence confirmed our specimen as C. trachodes. Morphologically, Clarkeinda trachodes BO24637 can be determined by a large basidiocarp of Agaricales, prominent pellicle on disc pileus, notable ring, reddish brown context and stipe when injured, and truncate apex of basidiospores. The BLAST result showed that our specimen has a high similarity to C. trachodes as the top hits. In addition, the phylogenetic tree constructed by ITS sequence committed specimen BO24637 as C. trachodes with 100% BS value. The current study resolves a clear and accessible document of C. trachodes in Indonesia. In addition, we provided the morphological and molecular information as well as herbarium collection of C. trachodes in Indonesia, which can be used for future study of this species.