PRODUCTION AND UTILIZATION OF CELLULASE FROM TRICHODERMA VIRIDE

Authors

  • SURYAHADI SURYAHADI Department of Animal Nutrition and Feed Science, Bogor Agricultural University,
  • R. HIDAYAT Department of Animal Nutrition and Feed Science, Padjadjaran University, Bandung, Indonesia.
  • R. HIDAYAT Department of Animal Nutrition and Feed Science, Padjadjaran University, Bandung, Indonesia.
  • S. WULANDARI Faculty of Animal Science, Jember Agricultural Poly technique, Jember, Indonesia
  • S. WULANDARI Faculty of Animal Science, Jember Agricultural Poly technique, Jember, Indonesia
  • K.G. WIRYAWAN Department of Animal Nutrition and Feed Science, Bogor Agricultural University, Bogor, Indonesia.
  • K.G. WIRYAWAN Department of Animal Nutrition and Feed Science, Bogor Agricultural University, Bogor, Indonesia.

DOI:

https://doi.org/10.11598/btb.2005.0.25.208

Abstract

  An appropriate preservation technology for forage such as silage needs to be developed in order to overcome the shortage of tropical forage during the dry season. A good quality of silage is obtained by decreasing the fibre contents (Neutral Detergent Fibre, Acid Detergent Fibre and lignin). The research was conducted in two stages: 1). Production and activity test of crude enzymes from Trichoderma viride and 2). Comparative test using crude enzymes from  Trichoderma viride strain QM 9414 (CETV) and commercial cellulase (Cellulase “Onozuka R-10â€, heidelberg) on rice straw silage. The treatments were arranged as follows : P0=Untreated rice straw silage, P1=Treated with commercial cellulase at 10 IU kg-1, P2= Treated with CETV at 3.8 IU kg-1, P3=Treated with CETV at 7.6 IU kg-1  and P4= Treated with CETV at 11.4 IU kg-1 of fresh rice straw. All treatments were enriched with 5% molasses. The result of the research indicated that: 1). The activity of crude enzymes from Trichoderma viride strain QM 9414 (CETV) was 1.52 IUml-1. 2). The addition of both enzymes did not decrease dry matter of rice straw silage; however, organic matter significantly decreased (p<0.05) by addition of commercial cellulase. 3). The addition of both enzymes decreased fibre contents (NDF), furthermore increased (p<0.05)  in sacco digestibility of dry matter and organic matter of rice straw silage.  Keywords  :  Cellulase/Trichoderma viride/Rice straw/Silage/Fibre

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