INHIBITORY EFFECT OF ULIN WOOD LIQUID SMOKE AND GOGO RICE ENDOPHYTIC FUNGI AGAINST PATHOGEN Pyricularia oryzae
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Diseases in rice plants (paddy) caused by microorganisms such as Pyricularia oryzae lead to a decrease in rice production. Therefore, it is essential to find out biological agents for protecting paddy and plants in general, against plant diseases. Liquid smoke and endophytic fungi have been known as biological agents to enhance the protection of plants against disease. The purpose of this study was to determine the ability of liquid smoke, endophytic fungi and the concentrations combinations to suppress the growth of P. oryzae. The results showed that liquid smoke concentrations of 0.17% to 1.75% and endophytic fungi filtrate of 2% to 10% showed significant ability against pathogen P. oryzae. However, the combination of liquid smoke and endophytic fungi filtrate at selected concentrations (0.17% liquid smoke combined with 2% endophytic fungi filtrate and 0.34% liquid smoke combined with 2% endophytic fungi filtrate) showed no significant inhibition percentage against P. oryzae compared to control. In conclusion, this study showed that the respective applications of liquid smoke and endophytic fungi filtrate inhibit the growth of P. oryzae.
Andriani Y. 2008. Budidaya Tanaman Padi di Indonesia. Jakarta (ID): Sastra Hudaya.
Barnett HL, Hunter BB. 1998. Illustrated Genera of Imperfect Fungi (4th ed.). St. Paul (US): American Phytopathological Society (APS Press). Available from: https://www.academia.edu/35499449/Illustrated_genera_of_imperfect_fungi_fourth_edition_Barnett_y_Hunter_pdf_pdf
BPPP Jateng. 2014. Kumpulan Deskripsi Varietas Padi. Semarang (ID): Balai Penelitian dan Pengembangan Pertanian.
Darmadji P. 2002. Optimasi pemurnian asap cair dengan metode redistilasi. Jurnal Teknologi Industri Pangan 13:267–271.
Hairmansis A, Yullianida Y, Supartopo S, Suwarno S. 2017. Rice improvement for upland areas. Iptek Tanaman Pangan 11(2):95–106. Available from: http://www.ejurnal.litbang.pertanian.go.id/index.php/ippan/article/view/6078
Hayashi K, Yoshida T, Hayano-Saito Y. 2019. Detection of white head symptoms of panicle blast caused by Pyricularia oryzae using cut-flower dye. Plant Methods 15(1):1–9.
Imaningsih W, Mariana, Junaedi AB, Rasyidah. 2021. Antifungal activities of the combination of ulin wood liquid smoke and Hiyung cayenne pepper root endophyte fungi against Colletotrichum capsici. Agrivita 43(1):69–78. https://doi.org/10.17503/agrivita.v1i1.2458
Junaidi AB, Apriyani H, Abdullah, Santoso UT. 2019. Fraksinasi dan karakterisasi asap cair dari kayu ulin (Eusideroxylon zwageri Teijsm. & Binn.) sebagai pelarut kitosan. Jurnal Riset Industri Hasil Hutan 11(2):53–64. https://doi.org/10.24111/jrihh.v11i2.4861
Junaidi AB, Nursyifa A, Abdullah. 2020. Redistillation and characterization of liquid smoke from ulin wood (Eusideroxylon zwageri Teijsm. & Binn.) and its ability as a chitosan solvent. IOP Conference Series: Materials Science and Engineering 980(1):012024. https://doi.org/10.1088/1757-899X/980/1/012024
Karseno P, Darmadji, Kapti R. 2001. Daya hambat asap cair kayu karet terhadap bakteri pengkontaminan lateks dan ribbed smoke sheet. Agritech 21(1):10–15.
Lalngaihawmi, Banik S, Chakruno P. 2019. Isolation of fungal endophytes of rice and their antagonistic effect against important rice fungal pathogens in vitro. Journal of Pharmacognosy and Phytochemistry 8(4):649–653.
Lee SH, H’ng PS, Chow MJ, Sajap AS, Tey BT, Salmiah U, Sun YL. 2011. Effectiveness of pyroligneous acid from vapour released in charcoal industry against biological attacks under laboratory conditions. Journal of Applied Sciences 11(24):3848–3853.
Luqman, Rizalinda, Khotimah S. 2015. Jamur mikoriza vesikular arbuskular (MVA) pada rhizosfer tanaman langsat (Lansium domesticum Corr.) di lahan gambut. Protobiont 4(3):89–97.
Manurung IR, Pinem MI, Lubis L. 2014. Uji antagonisme jamur endofit terhadap Cercospora oryzae Miyake dan Curvularia lunata (Wakk.) Boed. dari tanaman padi di laboratorium. Jurnal Online Agroekoteknologi 2(4):1563–1571.
Naik BS, Shashikala J, Krishnamurthy YL. 2009. Study on the diversity of endophytic communities from rice (Oryza sativa L.) and their antagonistic activities in vitro. Journal of Microbiology Research 164:290–296.
Norsalis E. 2011. Padi gogo dan sawah. Jurnal Online Agroekoteknologi 1(2):1–6.
Nurzannah SE, Lisnawita, Bakti D. 2014. Potensi jamur endofit asal cabai sebagai agens hayati untuk mengendalikan layu fusarium (Fusarium oxysporum). Jurnal Online Agroekoteknologi 2(3):1230–1238.
Oramahi HA, Diba F, Wahdina. 2011. Aktivitas antijamur asap cair dari serbuk gergaji kayu akasia (Acacia mangium Willd.) dan kayu laban (Vitex pubescens Vahl). Bionatura 13(1):79–84.
Oramahi HA, Yoshimura T. 2013. Antifungal and antitermitic activities of wood vinegar from Vitex pubescens Vahl. Journal of Wood Science 59:344–350.
Pszczola DE. 1995. Tour highlights production and uses of smoke-based flavors. Food Technology 49(1):70–74.
Rabha AJ, Naglot A, Sharma GD, Gogoi HK, Veer V. 2014. In vitro evaluation of antagonism of endophytic Colletotrichum gloeosporioides against fungal pathogens of Camellia sinensis. Indian Journal of Microbiology 54(3):302–309.
Sayer WJ, Shean DB, Ghosseiri J. 1969. Estimation of airborne fungal flora by the Andersen sampler versus gravity settling culture plate. Allergy 44(4):214–227. https://doi.org/10.1016/0021-8707(69)90088-4
Schulz B, Boyle C. 2005. The endophytic continuum. Mycological Research 109:661–687.
Singh A, Singh DK, Kharwar RN, White JF, Gond SK. 2021. Fungal endophytes as efficient sources of bioactive compounds. Microorganisms 9(1):1–42. https://doi.org/10.3390/microorganisms9010197
Soldera S, Sebastianutto N, Bortolomeazzi R. 2008. Composition of phenolic compounds and antioxidant activity of commercial aqueous smoke flavorings. Journal of Agricultural and Food Chemistry 56:2727–2734.
Suganda T, Yulia E, Widiantini F, Hersanti. 2016. Intensitas penyakit blas (Pyricularia oryzae Cav.) pada padi varietas Ciherang. Jurnal Agrikultura 27(3):154–159.
Talukdar D. 2011. Effect of arsenic-induced toxicity on morphological traits of Trigonella foenum-graecum L. and Lathyrus sativus L. Current Research Journal of Biological Sciences 2(3):116–123.
Ting ASY, Mah SW, Tee CS. 2010. Identification of volatile metabolites from fungal endophytes with biocontrol potential. American Journal of Agricultural and Biological Sciences 5(2):177–182.
Tomah AA, Abd Alamer IS, Li B, Zhang JZ. 2020. A new species of Trichoderma and gliotoxin role. Biological Control 145:104261. https://doi.org/10.1016/j.biocontrol.2020.104261
Wang X, Lee S, Wang J, Ma J, Bianco T, Jia Y. 2014. Current advances on genetic resistance to rice blast disease. In: Yan W, Bao J, editors. Rice Germplasm, Genetics and Improvement.
Waruwu AAS, Soekarno BPW, Munif A. 2016. Metabolit cendawan endofit tanaman padi sebagai alternatif pengendalian patogen terbawa benih. Jurnal Fitopatologi Indonesia 12(2):53–61.
White JF, Kingsley KL, Zhang Q, et al. 2019. Endophytic microbes and their potential applications in crop management. Pest Management Science 75(10):2558–2565. https://doi.org/10.1002/ps.5527
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