IN VITRO INOCULATION OF ASPARAGUS OFFICINALIS TISSUE CULTURE SHOOTS WITH FUSARIUM PROLIFERA TUM
Artificially inoculated asparagus tissue culture plantlets with a virulent fungus,Â Fusarium
proliferatum showed signs of infection as early as 4 days after inoculation. Macroscopic observations
revealed presence of early symptoms such as necrotic lesions at the affected area and light microscopic
examinations clearly revealed the post-penetration events that took place including the destruction of
surrounding cells. However, little is known of the hyphal activity or advancement on the host's surface at
the initial stage after inoculation. Scanning electron microscopic examination clearly revealed the hyphal
advancement on the surface and the mode of entrance into the host tissues beneath. Four days after
inoculation, the fungi proceeded to spread out from the inoculation point onto the host surface which
eventually developed into a sparse network of both aerial and non-aerial hyphae. Non-aerial hyphae form a
network of mycelium that adheres to the surface and it's movement appeared to be oriented towards the
stomata. Hyphal penetration occurs more often through the stomata, natural openings or wounds. In some
cases, the hyphae crossed over the stomatal opening without entering the host tissues. At places where the
cuticle layer is absent or not well developed the hyphae successfully grew in between the epidermal cells
into the tissues beneath.
Key words: Tissue culture/Asparagus officinalis/shoots/Artificial inoculstion/Fusarium proliferatum.
Download data is not yet available.
How to Cite
NORULAINI, N. N., SALLEH, B., ISKANDAR, R., & OMAR, A. M. (2011). IN VITRO INOCULATION OF ASPARAGUS OFFICINALIS TISSUE CULTURE SHOOTS WITH FUSARIUM PROLIFERA TUM. BIOTROPIA - The Southeast Asian Journal of Tropical Biology, (12). https://doi.org/10.11598/btb.19188.8.131.52
LicenseAuthors who publish with this journal agree with the following terms:
- Authors retain copyright and grant the journal right of first publication, with the work 1 year after publication simultaneously licensed under a Creative Commons attribution-noncommerical-noderivatesÂ 4.0 InternationalÂ License that allows others to share, copy and redistribute the work in any medium or format,Â but only where the use is for non-commercial purposesÂ and an acknowledgement of the work's authorship and initial publication in this journal is mentioned.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).