KARAMUNTING (Melastoma malabathricum) EXTRACTS ON WHITE SHRIMP (Litopenaeus vannamei) MATURITY
Keywords:Melastoma malabhtricum, Litopenaeus vannamei, Hormone progesterone, Histology Gonadal Maturity, Reproduction
AbstractLanosterol is a phytosterol that is used by crustacea to form animal steroid hormone that plays a role in the reproduction of the shrimp. This research aims to determine the response of ovary development in white shrimp parent after given Karamunting ethanol extract. This research was carried out in several stages: 1. Injecting the white shrimp every 3 days, at the base of the 5th leg, for 15 days with variable control dose 0 (C), 10 mg / kg BW (T1), 7.5 mg / kg BW (T2), 5 mg / kg BW (T3), 2 mg / kg BW (T4) and 1 mg / kg BW (T5), (2). Isolation of white shrimp parent ovary, (3). Measurement of progesterone level in the ovary using the Radioimmunoassay (RIA) method, (4) Histology of white shrimp parent ovary, and (5). Data analysis. The statistical results of measuring the increase in progesterone showed that administration of ethanol extract of karamunting at the end of maintenance had a significant difference (PË‚0.05). Histology observations of gonadal development in control, T5 and T4 showed cells to develop to previtellogenesis oocytes whereas in treatment T1, T2 and T3 ovary cells developed into endogenous vitellogenesis oocytes and only in T1 ovarian cells developed to form exogenous vitellogenesis oocytes. Â Statistical results of oocyte size after administration of the karamunting extract at the end of maintenance were significantly different (PË‚0.05). The average oocyte size at the beginning of maintenance (15.57 Â± 3.15 Âµm), C (25.29 Â± 2.69 Âµm) indicating that at the end of the ovarian treatment development occurred both in control and treatment. T1 (65.65 Â± 2.64 Âµm), T2 (63.98 Â± 3.06 Âµm), T3 (39.12 Â± 6.01 Âµm), T4 (28.08 Â± 0.84 Âµm) and T5 (27.65 Â± 0.71 Âµm) also appear to be greater than the oocyte size at the beginning of maintenance and control. Based on the results of this study it can be concluded that the content of lanosterol in karamunting plants can increase the hormone progesterone which indicates an acceleration of gonadal maturity and enlargement of oocyte size in the parent shrimp of White shrimp.
Download data is not yet available.
How to Cite
Ridwan, A., & Awaludin, A. (2021). KARAMUNTING (Melastoma malabathricum) EXTRACTS ON WHITE SHRIMP (Litopenaeus vannamei) MATURITY. BIOTROPIA - The Southeast Asian Journal of Tropical Biology, 28(2). https://doi.org/10.11598/btb.0.0.0.1297
Copyright (c) 2020 BIOTROPIA - The Southeast Asian Journal of Tropical Biology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.Authors who publish with this journal agree with the following terms:
- Authors retain copyright and grant the journal right of first publication, with the work 1 year after publication simultaneously licensed under a Creative Commons attribution-noncommerical-noderivatesÂ 4.0 InternationalÂ License that allows others to share, copy and redistribute the work in any medium or format,Â but only where the use is for non-commercial purposesÂ and an acknowledgement of the work's authorship and initial publication in this journal is mentioned.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).