KARAMUNTING (Melastoma malabathricum) EXTRACTS ON WHITE SHRIMP (Litopenaeus vannamei) MATURITY
Keywords:Melastoma malabhtricum, Litopenaeus vannamei, Hormone progesterone, Histology Gonadal Maturity, Reproduction
AbstractLanosterol is a phytosterol that is used by crustacea to form animal steroid hormone that plays a role in the reproduction of the shrimp. This research aims to determine the response of ovary development in white shrimp parent after given Karamunting ethanol extract. This research was carried out in several stages: 1. Injecting the white shrimp every 3 days, at the base of the 5th leg, for 15 days with variable control dose 0 (C), 10 mg / kg BW (T1), 7.5 mg / kg BW (T2), 5 mg / kg BW (T3), 2 mg / kg BW (T4) and 1 mg / kg BW (T5), (2). Isolation of white shrimp parent ovary, (3). Measurement of progesterone level in the ovary using the Radioimmunoassay (RIA) method, (4) Histology of white shrimp parent ovary, and (5). Data analysis. The statistical results of measuring the increase in progesterone showed that administration of ethanol extract of karamunting at the end of maintenance had a significant difference (PË‚0.05). Histology observations of gonadal development in control, T5 and T4 showed cells to develop to previtellogenesis oocytes whereas in treatment T1, T2 and T3 ovary cells developed into endogenous vitellogenesis oocytes and only in T1 ovarian cells developed to form exogenous vitellogenesis oocytes. Â Statistical results of oocyte size after administration of the karamunting extract at the end of maintenance were significantly different (PË‚0.05). The average oocyte size at the beginning of maintenance (15.57 Â± 3.15 Âµm), C (25.29 Â± 2.69 Âµm) indicating that at the end of the ovarian treatment development occurred both in control and treatment. T1 (65.65 Â± 2.64 Âµm), T2 (63.98 Â± 3.06 Âµm), T3 (39.12 Â± 6.01 Âµm), T4 (28.08 Â± 0.84 Âµm) and T5 (27.65 Â± 0.71 Âµm) also appear to be greater than the oocyte size at the beginning of maintenance and control. Based on the results of this study it can be concluded that the content of lanosterol in karamunting plants can increase the hormone progesterone which indicates an acceleration of gonadal maturity and enlargement of oocyte size in the parent shrimp of White shrimp.
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