INFLUENCE OF MEDIA GELLING AGENTS ON ROOT BIOMASS AND IN VITRO VA-MYCORRfflZAL SYMBIOSIS OF CARROT WITH GIGASPORA MARGARITA

Authors

  • NAVEEN PAL BHATIA Tata Energy Research Institute, Durban Seth Block, Habitat Place, Lodi Road
  • ALOK ADHOLEYA Tata Energy Research Institute, Durban Seth Block, Habitat Place, Lodi Road, New Delhi 110 003, India
  • ANJALI VERMA Tata Energy Research Institute, Durban Seth Block, Habitat Place, Lodi Road, New Delhi 110 003, India

DOI:

https://doi.org/10.11598/btb.1997.0.10.128

Abstract


An in vitro study with Ri-TDNA transformed roots of carrot  (Daucus carota) was carried out  to evaluate  the role of
macro-elements contributed as impurities in  the gelling agent (phytagel) over and above  those present  in the minimal (M)
medium. Production of root biomass was taken as a measure to quantify the influence of macro-elements added to the minimal
medium. The levels of phosphorus when adjusted to 1.19 mg/1 and 1.09 mg/l, lead to dry root biomass production at par with
the control.
Attempts made to lower the amount of impurities in phytagel by de-ionization using different alkalies, proved NaOH to
give the best results in terms of relatively high amount of root biomass. In an in vitro dual culture system with carrot as host
and Gigaspora margarita as the vesicular-arbuscular mycorrhizal fungus, phytagel impurities helped to produce maximum
number of infection units and auxiliary cells when phytagel was added to the minimal medium.

Key words:    Agrobacterium    rhiiogenesfDaucus    caro/a/Gelling    agents/diaspora    margarita/Macro-
elements/Vesicular-arbuscular mycorrhiza/Transformed roots.

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How to Cite

PAL BHATIA, N., ADHOLEYA, A., & VERMA, A. (2011). INFLUENCE OF MEDIA GELLING AGENTS ON ROOT BIOMASS AND IN VITRO VA-MYCORRfflZAL SYMBIOSIS OF CARROT WITH GIGASPORA MARGARITA. BIOTROPIA - The Southeast Asian Journal of Tropical Biology, (10). https://doi.org/10.11598/btb.1997.0.10.128

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Section

Research Paper