INFLUENCE OF MEDIA GELLING AGENTS ON ROOT BIOMASS AND IN VITRO VA-MYCORRfflZAL SYMBIOSIS OF CARROT WITH GIGASPORA MARGARITA
Key words: Agrobacterium rhiiogenesfDaucus caro/a/Gelling agents/diaspora margarita/Macro-
An in vitro study with Ri-TDNA transformed roots of carrot (Daucus carota) was carried out to evaluate the role of
macro-elements contributed as impurities in the gelling agent (phytagel) over and above those present in the minimal (M)
medium. Production of root biomass was taken as a measure to quantify the influence of macro-elements added to the minimal
medium. The levels of phosphorus when adjusted to 1.19 mg/1 and 1.09 mg/l, lead to dry root biomass production at par with
Attempts made to lower the amount of impurities in phytagel by de-ionization using different alkalies, proved NaOH to
give the best results in terms of relatively high amount of root biomass. In an in vitro dual culture system with carrot as host
and Gigaspora margarita as the vesicular-arbuscular mycorrhizal fungus, phytagel impurities helped to produce maximum
number of infection units and auxiliary cells when phytagel was added to the minimal medium.
elements/Vesicular-arbuscular mycorrhiza/Transformed roots.
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