SOURCES OF MYCORRHIZAL INFECTION OF SHOREA ACUMINATA SEEDLINGS UNDER LABORATORY CONDITIONS*)
Uninoculated dipterocarp seedlings raised in normal field soil in nurseries were always found to have
mycorrhizas after a few months. This study set out to determine whether dipterocarp seedlings could continue
to grow and develop in the absence of mycorrhizas and also to determine possible sources of mycorrhizal infection
ofÂ dipterocarpÂ seedlingsÂ raisedÂ underÂ laboratoryÂ conditionsÂ usingÂ ShoreaÂ acuminataÂ asÂ aÂ typicalÂ example.
Seedlings were planted in capped or uncapped perspex boxes containingÂ sterile or non-sterileÂ field soil and
wateredÂ dailyÂ withÂ sterileÂ waterÂ orÂ tapÂ water.Â SeedlingÂ growthÂ andÂ developmentÂ ofÂ mycorrhizasÂ were
monitored at monthly intervals for up to seven months. Seedlings grown in sterile soil remained uninfected after
seven months while infection was found in some of the seedlings grown in normal soil regardless of whether they
had been watered with tap water or sterile water. This showed that field soil (i.e. under grass) far from the forest
contained suitable inoculum for forest tree seedlings. Tap water and the air were not important sources of infection.
However, mycorrhizalÂ infection wasÂ veryÂ unevenÂ indicatingÂ thatÂ theÂ inoculum wasÂ probablyÂ veryÂ unevenly
distributed in the soil or that the inoculum density was rather low. Seedlings grown in sterile soil showed better
growthÂ thanÂ thoseÂ grownÂ inÂ normalÂ soilÂ andÂ infectionÂ ofÂ rootsÂ byÂ parasiticÂ fungiÂ inÂ theÂ latter wasÂ also
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