DIVERSITY OF ACTINOMYCETES FROM EKA KARYA BOTANICAL GARDEN, BALI

Authors

  • Shanti Ratnakomala Research Center for Biotechnology Indonesian Institute of Sciences
  • Puspita Lisdiyanti Research Center for Biotechnology Indonesian Institute of Sciences
  • Nita Rosalinda Prayitno University Health Network - Toronto General Hospital
  • Evi Triana Research Center for Biology Indonesian Institute of Sciences
  • Yulin Lestari Departement of Biology, Faculty of Mathematics and Natural Sciences, IPB, Darmaga Campus, Bogor
  • Ratih Dwi Hastuti Soil Research Institute, Bogor 16002
  • Yantyati Widyastuti Research Center for Biotechnology Indonesian Institute of Sciences
  • Misa Otoguro University of Yamanashi
  • Katsuhiko Ando NITE Biologycal Resource Center (NBRC)
  • Endang Sukara Research Center for Biotechnology Indonesian Institute of Sciences

DOI:

https://doi.org/10.11598/btb.2016.23.1.504

Abstract

A total of 229 strains of actinomycetes were isolated and identified by full sequence of 16S rRNA gene analysis. Samples consisted of 18 soil and 20 leaf-litter were collected from Eka Karya Botanical Garden, Bali Island, Indonesia. Two isolation methods, i.e. SDS-Yeast Extract (SY) and Rehydration-Centrifugation (RC) were used in this study. Based on 16S rRNA gene analysis, isolated actinomycetes may be grouped into 28 genera. Based on molecular analysis of 16S rRNA gene similarities showed that isolated actinomycetes of Eka Karya Botanical Garden origin is diverse. Analysis on 144 isolates from soil samples, resulted in 24 genera and more than 87 species. Streptomyces is the most dominant genus where 65 isolates or 45% from isolated actinomycetes belong to this genus. It was followed by Actinoplanes (25 isolates =17%). From leaf-littersamples, the total number of 85 isolates may be grouped into 9 genera and more than 41 species. The most dominated genus is Actinoplanes (42 isolates =49%) followed by Catenuloplanes (16 isolates=19%).

Keywords: 16S rRNA gene analysis, actinomycetes, biodiversity, Eka Karya Botanical Garden

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Published

2016-08-03

How to Cite

Ratnakomala, S., Lisdiyanti, P., Prayitno, N. R., Triana, E., Lestari, Y., Hastuti, R. D., Widyastuti, Y., Otoguro, M., Ando, K., & Sukara, E. (2016). DIVERSITY OF ACTINOMYCETES FROM EKA KARYA BOTANICAL GARDEN, BALI. BIOTROPIA, 23(1), 42–51. https://doi.org/10.11598/btb.2016.23.1.504

Issue

Vol. 23 No. 1 (2016)

Section

Research Paper

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