PHYSIOLOGICAL AND METABOLOMIC APPROACH FROM DIFFERENT ACCESSIONS OF MULBERRY TREATED WITH SALT STRESS IN INDONESIA
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ARTICLE HIGHLIGHTS
- Information on salinity-tolerant mulberry accessions is currently unavailable. This research reveals the discovery of salinity-tolerant mulberry accessions from the exploration of various mulberry accessions in Indonesia.
- Salinity-tolerant mulberry accessions can be utilized for sustainable development and biodiversity conservation.
- Salinity stress treatment is known to increase secondary metabolites in mulberry plants.
- Profiling of secondary metabolites in mulberry plants in response to salinity stress is known.
- The integration of metabolomics and ecophysiology offers a new perspective on how plants adapt to stress.
ABSTRACT
Land salinity in Indonesia continues to increase, therefore research is needed to obtain saline-resistant plants such as mulberries. Mulberry plants are well recognized for their resilience to various abiotic stresses, including salinity. This study aimed to identify tolerant accessions to salinity stress, in accordance with their growth characteristics, and to examine the alterations in secondary metabolites as a reaction to salt stress. The experiment was designed using a factorial randomized block design, which included seven mulberry accessions, identified as MB1 through MB7, and varying NaCl concentrations of 0% (control), 0.2%, 0.3%, and 0.4%. The study’s results showed that MB2-3 accession tolerates high salinity stress by accumulating proline, flavonoid compounds, phenolic and dissolved sugars, as well as secondary metabolites, with increasing NaCl concentration. Based on the results of UHPLC-Q-Orbitrap HRMS analysis of mulberry leaf extract, 100 selected metabolites were obtained. The heat map results showed metabolic substances such as phenylpropanoids, organic acids, lipids, and carbohydrates increased in response to salinity stress. The integration of metabolomics and ecophysiology called ecophysiolomics offers a new perspective on how plants adapt to stress. By analyzing the involvement of metabolites that facilitate tolerance to salinity stress, we can gain a valuable understanding of how mulberry plants endure this environmental challenge.
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Copyright (c) 2026 Yasinta Ratna Esti Wulandari, Yohana Caecilia Sulistyaningsih, Agik Suprayogi, Min Rahminiwati, Triadiati Triadiati
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