GENETIC DIVERSITY OF AMPICILLIN-RESISTANT Vibrio ISOLATED FROM VARIOUS STAGES OF TIGER SHRIMP LARVAE DEVELOPMENT
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Abstract
This research was carried out to study genetic diversity of ampicillin-resistant Vibrio from
various stages of tiger shrimp larvae (Penaeus Monodon) development from,Tambak Inti Rakyat
hatchery, near Labuan, West Java, Indonesia. A total of 25 ampicillin-resistant Vibrio isolates were
isolated using thiosulphate citrate bile-salt sucrose agar (TCBS-Agar) and seawater complete
agar (SWC-Agar). Physiological and biochemical characterization showed that the isolates could be
grouped into only two species, i.e. V. harveyi from the egg stage; and V. metschnikovii from larvae
and post-larval stage (i.e nauplius, zoea, mysis, PLi, PL5, PL,0, and PL,5). These isolates were also
present in their respective rearing water of each stage and some natural feed. Schizotyping
analysis employing restriction endonuclease Noll (5'-GC4GGCCGC) indicated that the isolates
could be grouped into at least 13 different genotypes. Therefore, schizotyping was more
discriminative than physiological characterization. This study showed that particular groups of Vibrio
colonized all stages of shrimp larvae and demonstrated closed phylogenetic relationship. These groups
of Vibrio might be the dominant microbiota which could suppress the development of other Vibrio
including the pathogenic Vibrio.
Key words : Shrimp/ampicillin-resistant K/fcno/schizotyping
various stages of tiger shrimp larvae (Penaeus Monodon) development from,Tambak Inti Rakyat
hatchery, near Labuan, West Java, Indonesia. A total of 25 ampicillin-resistant Vibrio isolates were
isolated using thiosulphate citrate bile-salt sucrose agar (TCBS-Agar) and seawater complete
agar (SWC-Agar). Physiological and biochemical characterization showed that the isolates could be
grouped into only two species, i.e. V. harveyi from the egg stage; and V. metschnikovii from larvae
and post-larval stage (i.e nauplius, zoea, mysis, PLi, PL5, PL,0, and PL,5). These isolates were also
present in their respective rearing water of each stage and some natural feed. Schizotyping
analysis employing restriction endonuclease Noll (5'-GC4GGCCGC) indicated that the isolates
could be grouped into at least 13 different genotypes. Therefore, schizotyping was more
discriminative than physiological characterization. This study showed that particular groups of Vibrio
colonized all stages of shrimp larvae and demonstrated closed phylogenetic relationship. These groups
of Vibrio might be the dominant microbiota which could suppress the development of other Vibrio
including the pathogenic Vibrio.
Key words : Shrimp/ampicillin-resistant K/fcno/schizotyping
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WIDANARNI, W., & SUWANTO, A. (2011). GENETIC DIVERSITY OF AMPICILLIN-RESISTANT Vibrio ISOLATED FROM VARIOUS STAGES OF TIGER SHRIMP LARVAE DEVELOPMENT. BIOTROPIA, (15). https://doi.org/10.11598/btb.2000.0.15.160
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