ISOLATING MICROSATELLITE FROM Amorphophallus variabilis AND ITS APPLICATION FOR POPULATION STUDY IN DRAMAGA CONSERVATION FOREST, INDONESIA

Edi Santosa, Chun Lan Lian, Yoko Mine, Ken Takahata, Nobuo Sugiyama

Abstract


Amorphophallus variabilis Blume, a member of Araceae, is a fleshy perennial tuber crop endemic in Java Island, Indonesia. The plant produces white edible corm; and it was used as food during famine time before 1960s. Rapid ecological changes and land fragmentations in Java in recent times threaten populations of A. variabilis. Here, compound microsatellite markers were developed in order to develop conservation strategies in the populations. Twelve primers pairs produced high polymorphism ranging from 5 to 22 alleles per locus. The observed and expected heterozygosities ranged from 0.191 to 0.851 and 0.380 to 0.943, respectively. This high allelic diversity indicates that these markers are suitable for the study on population genetic structure. Cross-amplification on related and non-related species was performed. Application of the markers on populations from Dramaga Conservation Forest revealed high allelic richness, high diversity within and among populations. Genetic distance among populations increased with an increase of geographic distance. Present study suggested that, it is important to study population of A. variabilis in Java in order to understand the population genetic structure and develop effective in situ conservation programs.

Keywords


Araceae, genetic population, SSR, tuber crop, white iles-iles

Full Text:

PDF


DOI: http://dx.doi.org/10.11598/btb.2018.25.1.652

Copyright (c) 2018 BIOTROPIA - The Southeast Asian Journal of Tropical Biology

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

SEAMEO BIOTROP