BIOTROPIA
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description: BIOTROPIA, The Southeast Asian Journal of Tropical Biology (p-ISSN 0215-6334, e-ISSN 1907-770X), is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology. Since 2012, BIOTROPIA has been indexed by SciVerse SCOPUS (Elsevier), CrossRef, DOAJ and Google Scholar. Articles published in the journal are papers reporting results of original research, reviews on specific topics not available elsewhere in up-to-date form, and short communication in which advances in knowledge of significance are briefly announced. Submission of paper is taken to imply that it has not been submitted to other journals and that, if accepted, will not be published elsewhere. All papers submitted for publication in BIOTROPIA are evaluated by subject matter experts and go through editorial revision. Authors may suggest names of potential reviewers. BIOTROPIA uses anonymous reviewing, in which the identities of authors and reviewers are kept confidential.
publisher: SEAMEO BIOTROP
language: en
publication name: BIOTROPIA
issn: 0215-6334
copyright: Authors who publish with this journal agree with the following terms: Authors retain copyright and grant the journal right of first publication, with the work 1 year after publication simultaneously licensed under a Creative Commons attribution-noncommerical-noderivates 4.0 International License that allows others to share, copy and redistribute the work in any medium or format, but only where the use is for non-commercial purposes and an acknowledgement of the work's authorship and initial publication in this journal is mentioned. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
items: CHARACTERISTICS OF GOLDEN APPLE SNAIL BIOSTIMULANTS AND AGRONOMIC EFFECTIVENESS TESTS ON THE COFFEE SEEDLINGS
items: UPDATED CHECKLIST OF HERPETOFAUNA IN BALINSASAYAO TWIN LAKES NATURAL PARK, NEGROS ORIENTAL: A LONG-TERM ECOLOGICAL RESEARCH SITE IN THE PHILIPPINES
items: ETHNOBOTANICAL, NUTRITIONAL, AND FUNCTIONAL PROSPECTS OF INDONESIAN MANGROVE APPLES (Sonneratia spp.)
items: HERPETOFAUNAL COMMUNITIES IN BUTUAN CITY, PHILIPPINES: A SATOYAMA-SATOUMI APPROACH TO BIODIVERSITY ASSESSMENT
items: PHYSIOLOGICAL AND METABOLOMIC APPROACH FROM DIFFERENT ACCESSIONS OF MULBERRY TREATED WITH SALT STRESS IN INDONESIA
items: AGRONOMIC AND GRAIN YIELD TRAITS OF RICE VARIETIES UNDER SEASONAL VARIATION IN TROPICAL LOWLANDS
items: NATURE-BASED SOLUTIONS FOR RESILIENT RICE AGRO-ECOSYSTEMS
items: WILD IN THE CITY: UNAUTHORIZED POSSESSION OF WILD FAUNA IN RESIDENCES OF VALENCIA CITY, BUKIDNON, PHILIPPINES
items: OPTIMIZING eDNA METABARCODING IN INDONESIAN FRESHWATERS: A SCOPING REVIEW OF BEST PRACTICES
items: CARBON STOCK ESTIMATION IN FABACEAE TREE STANDS WITHIN THE URBAN FORESTSOF BANDA ACEH
items: YEAST EXTRACT ELICITATION ENHANCES METABOLITE PRODUCTION IN Alocasia cuprea CALLUS
items: ETHNOBOTANICAL STUDY OF MANGROVES FROM COASTAL COMMUNITIES OF BUNAKEN NATIONAL PARK, INDONESIA
items: DIVERSITY OF SMALL NON-VOLANT MAMMALS IN THE CENTER FOR ECOLOGICAL DEVELOPMENT AND RECREATION (CEDAR), BUKIDNON, PHILIPPINES
items: HEPATOPROTECTIVE EFFECT OF Physalis angulata L. LEAF ETHANOL EXTRACT AGAINST BUSULFAN-INDUCED LIVER DAMAGE IN FEMALE RATS
items: RELATIONSHIP BETWEEN FIBER MORPHOLOGY AND PARALLEL-TO-GRAIN MECHANICAL STRENGTH IN FOUR BAMBOO SPECIES GROWNIN THE PHILIPPINES

CHARACTERISTICS OF GOLDEN APPLE SNAIL BIOSTIMULANTS AND AGRONOMIC EFFECTIVENESS TESTS ON THE COFFEE SEEDLINGS
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description: ARTICLE HIGLIGHTS - Golden apple snail extract was successfully developed into a biostimulant containing NPK nutrients, 12 amino acids, and indole-3-acetic acid (IAA). - The biostimulant significantly enhanced coffee seedling growth, including plant height, root length, leaf area, chlorophyll content, and biomass. - Golden apple snail biostimulant achieved the highest Relative Agronomic Effectiveness (RAE) of 104%, outperforming conventional NPK fertilization. - Formulation without EM4 retained higher concentrations of macronutrients and amino acids, whereas EM4 supplementation increased IAA production. - Utilizing an invasive golden apple snail as a plant biostimulant provides a sustainable alternative to inorganic fertilizers for coffee nurseries. ABSTRACT The use of Golden Apple Snail (GAS), an invasive species, as a potential biostimulant source represents an innovation, particularly in sustainable agriculture and waste management. Therefore, this study aimed to determine the biochemical characteristics of GAS and assess its effectiveness on coffee seedling growth. The first test, detecting biochemical characteristics, was conducted using two formulations, i.e., 1) Formulation 1 = 1,000 g of fresh whole GAS extract + 500 g of sugar and 2) Formulation 2 = 750 g of fresh whole GAS extract + 500 g of sugar + 125 mL of EM 4. The results of the 14-day fermentation showed the presence of nutrients N, P, K, 12 amino acids, and phytohormones. Formulation 1 had a total amount of N-Total (1.76%), P2O5 (0.18%) and K2O (0.29%) and a higher amino acid content than those in Formulation 2. In phytohormone detection, IAA was present in both formulations. The results of this test were used for further tests on the growth of Robusta coffee seedlings. The second test of agronomic effectiveness was carried out using a Randomized Block Design, with 6 types of fertilization treatments: 1) Control (Without GAS and NPK Biostimulants); 2) GAS Biostimulant 50 mL/L; 3) GAS Biostimulant 50…
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creator: Sepdian Luri Asmono
creator: Rahmawati
creator: Nisa Budi Arifiana
creator: Dyah Nuning Erawati
creator: Nurul Sjamsijah
creator: N. Bambang Eko Sulistyono
rights: Copyright (c) 2026 Sepdian Luri Asmono, Rahmawati, Nisa Budi Arifiana, Dyah Nuning Erawati, Nurul Sjamsijah, N. Bambang Eko Sulistyono http://creativecommons.org/licenses/by-nc-nd/4.0
date: 2026-08-03
publication date: 2026-08-03
starting page: 453
ending page: 463
doi: 10.11598/btb.2026.33.3.2466

UPDATED CHECKLIST OF HERPETOFAUNA IN BALINSASAYAO TWIN LAKES NATURAL PARK, NEGROS ORIENTAL: A LONG-TERM ECOLOGICAL RESEARCH SITE IN THE PHILIPPINES
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description: ARTICLE HIGLIGHTS - Updated checklist reveals rich amphibian and reptile diversity. - Twenty-two endemic species found occur only in the Philippines. - Seven threatened species highlight the area's conservation importance. - Species richness is comparable with other long-term research sites. - Continued monitoring may reveal more amphibian and reptile species. ABSTRACT The Balinsasayao Twin Lakes Natural Park (BTLNP) is one the established Long-term Ecological Research Sites (LTER) in the Philippines. LTER sites are valuable for providing underlying causes for changes in the environment, management of natural resources, and conservation of biodiversity. Through this, researchers could quantify simultaneously certain complex ecological interactions. This primary aim of this study is to assess the herpetofauna to update the existing data on herps, and compare its species richness with other LTER sites. Survey was done by using the cruising method in the established 2-hectare plot, and other areas outside of it. A total of three sampling visits with six field days was spent from the sampling visits on. The findings of this study included detecting 29 herpetofauna species, composed of: 11 amphibian and 18 reptile species, 17 of which are endemic and six are resident. This record represents 54.7% of the total number of 53 species listed by Dolino et al., 2004. There are five species described to be threatened, namely: Platymantis negrosensis, Platymantis hazelae, Limnonectes visayanus, Sanguirana acai, Hydrosaurus pustulatus, Tropidonophis negrosensis, and Oligodon modestus. In comparison with the other LTER sites, albeit having a completely different presence of species, the level of species richness at BTLNP is close to Mt. Hamiguitan. This implies that the habitat is similar in conditions, and the demands of each species are well provided. The presence of the lake itself might be the major reason as it can offer a variety of resources for these species. Many egg layin…
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creator: Jean Henri Oracion
creator: Arnold Rabor
creator: Sharmaine Arjona
creator: Victor Amoroso
rights: Copyright (c) 2026 Jean Henri Oracion, Arnold A. Rabor, Sharmaine P. Arjona, Victor B. Amoroso http://creativecommons.org/licenses/by-nc-nd/4.0
date: 2026-08-22
publication date: 2026-08-22
starting page: 438
ending page: 452
doi: 10.11598/btb.2026.33.3.2513

ETHNOBOTANICAL, NUTRITIONAL, AND FUNCTIONAL PROSPECTS OF INDONESIAN MANGROVE APPLES (Sonneratia spp.)
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description: ARTICLE HIGHLIGHTS - Comprehensive review of Indonesian mangrove apple (Sonneratia spp.), focusing on S. alba, S. caseolaris, and S. ovata across major coastal regions. - Nutritional profile of Indonesian mangrove apple characterized by significant levels of vitamins, flavonoids, and organic acids with high bioactive potential. - Documented functional properties of Indonesian mangrove apple, include antioxidant, anti-inflammatory, anti-allergic, and antidiabetic activities. - Emphasizes the role of mangrove apple utilization in promoting supporting food security and public health, sustainable coastal ecosystem conservation and biodiversity preservation. ABSTRACT Mangrove apples (Sonneratia spp.), native to Indonesia’s extensive mangrove forests, is distributed across Sumatra, Kalimantan, Sulawesi, Maluku, and Papua. This review synthesizes ethnobotanical uses, nutritional attributes, and functional potential of three main species S. alba, S. caseolaris, and S. ovata that hold both ecological and cultural significance. A review article search was conducted following the PRISMA 2020 framework and structured in accordance with the standard IMRaD across Scopus, PubMed, ScienceDirect, Web of Science, and Google Scholar, yielding 91 eligible studies from an initial 612 records. Ethnobotanical evidence documents diverse applications in traditional foods, including chips, stick crackers, sweets such as dodol, syrup, candy, ice cream, and marshmallow, reflecting deep-rooted ties between coastal communities and mangrove ecosystems. Nutritional analyses highlight rich contents of vitamins and bioactive compounds such as flavonoids, and organic acids. These constituents exhibit antioxidant, anti-inflammatory, anti-allergic, and antidiabetic properties, underscoring the species’ promise as a functional food ingredient. By integrating traditional culinary heritage with scientific evidence, this review positions Sonneratia spp. as both a symbol of Indonesia’s coastal identity and …
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creator: Muhammad Agung Islamy
creator: Erika Meiliana Sari
creator: Olifia Mutiara Sandi
rights: Copyright (c) 2026 Muhammad Agung Islamy, Erika Meiliana Sari, Olifia Mutiara Sandi http://creativecommons.org/licenses/by-nc-nd/4.0
date: 2026-08-22
publication date: 2026-08-22
starting page: 494
ending page: 503
doi: 10.11598/btb.2026.33.3.2723

HERPETOFAUNAL COMMUNITIES IN BUTUAN CITY, PHILIPPINES: A SATOYAMA-SATOUMI APPROACH TO BIODIVERSITY ASSESSMENT
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description: ARTICLE HIGHLIGHTS - Community-managed landscapes and seascapes support endemic and native amphibian and reptile species - Forty-three amphibian and reptile species were recorded in Butuan City. - The landscape and seascape are dominated by lowland amphibian and reptile species. - This study provides baseline data for biodiversity conservation and monitoring. ABSTRACT This study provides the first systematic assessment of amphibians and reptiles within the Satoyama-Satoumi landscape-seascape mosaic of Butuan City, Agusan del Norte, Philippines. Herpetofaunal surveys were conducted across six habitat types in Barangays Tungao (agroforest, grassland-agroecosystem, riparian) and Masao (landward, middleward, and seaward mangroves) using habitat-stratified transects, diurnal and nocturnal searches, passive trapping, and standardized field recording. A total of 43 species were documented, comprising 14 amphibians and 29 reptiles, including 24 Philippine endemics, of which 13 species are Mindanao Faunal Region endemics. Three invasive amphibians were also recorded. Species diversity was higher in Tungao (H′ = 2.57) than in Masao (H′ = 1.94), reflecting contrasts in habitat condition and disturbance intensity. Most species were classified as Least Concern (n = 36). However, the presence of Near Threatened (n = 1) and nationally recognized Other Threatened Species (n = 11) highlights the conservation importance of the area. Distinct microhabitat associations from forest floors and riparian zones to mangroves and human-modified areas emphasize the ecological heterogeneity supporting these assemblages. This baseline inventory demonstrates that community-managed Satoyama–Satoumi habitats serve as important secondary refugia for herpetofauna and highlights the need for sustained monitoring and habitat protection in Butuan City.
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creator: Leonardo Estano
creator: Jela Rensulat
creator: Jeco Jed Ruales
creator: Inocencio E. Buot Jr.
creator: Jess H. Jumawan
rights: Copyright (c) 2026 Leonardo Estano, Jela Rensulat, Jeco Jed Ruales, Inocencio E. Buot Jr., Jess H. Jumawan http://creativecommons.org/licenses/by-nc-nd/4.0
date: 2026-08-21
publication date: 2026-08-21
starting page: 474
ending page: 493
doi: 10.11598/btb.2026.33.3.2792

PHYSIOLOGICAL AND METABOLOMIC APPROACH FROM DIFFERENT ACCESSIONS OF MULBERRY TREATED WITH SALT STRESS IN INDONESIA
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description: ARTICLE HIGHLIGHTS - Information on salinity-tolerant mulberry accessions is currently unavailable. This research reveals the discovery of salinity-tolerant mulberry accessions from the exploration of various mulberry accessions in Indonesia. - Salinity-tolerant mulberry accessions can be utilized for sustainable development and biodiversity conservation. - Salinity stress treatment is known to increase secondary metabolites in mulberry plants. - Profiling of secondary metabolites in mulberry plants in response to salinity stress is known. - The integration of metabolomics and ecophysiology offers a new perspective on how plants adapt to stress. ABSTRACT Land salinity in Indonesia continues to increase, therefore research is needed to obtain saline-resistant plants such as mulberries. Mulberry plants are well recognized for their resilience to various abiotic stresses, including salinity. This study aimed to identify tolerant accessions to salinity stress, in accordance with their growth characteristics, and to examine the alterations in secondary metabolites as a reaction to salt stress. The experiment was designed using a factorial randomized block design, which included seven mulberry accessions, identified as MB1 through MB7, and varying NaCl concentrations of 0% (control), 0.2%, 0.3%, and 0.4%. The study’s results showed that MB2-3 accession tolerates high salinity stress by accumulating proline, flavonoid compounds, phenolic and dissolved sugars, as well as secondary metabolites, with increasing NaCl concentration. Based on the results of UHPLC-Q-Orbitrap HRMS analysis of mulberry leaf extract, 100 selected metabolites were obtained. The heat map results showed metabolic substances such as phenylpropanoids, organic acids, lipids, and carbohydrates increased in response to salinity stress. The integration of metabolomics and ecophysiology called ecophysiolomics offers a new perspective on how plants adapt to stress. By analyzing the involvement of metabolites t…
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creator: Yasinta Ratna Esti Wulandari
creator: Yohana Caecilia Sulistyaningsih
creator: Agik Suprayogi
creator: Min Rahminiwati
creator: Triadiati Triadiati
rights: Copyright (c) 2026 Yasinta Ratna Esti Wulandari, Yohana Caecilia Sulistyaningsih, Agik Suprayogi, Min Rahminiwati, Triadiati Triadiati http://creativecommons.org/licenses/by-nc-nd/4.0
date: 2026-08-13
publication date: 2026-08-13
starting page: 464
ending page: 473
doi: 10.11598/btb.2026.33.3.2884

AGRONOMIC AND GRAIN YIELD TRAITS OF RICE VARIETIES UNDER SEASONAL VARIATION IN TROPICAL LOWLANDS
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description: ARTICLE HIGHLIGHTS - Planting time in the wet season changed rice growth and grain yield. - Mid rainy season planting reduced grain yield in all tested rice varieties. - Hybrid Intani 602 recorded the highest yield in late rainy season planting. - Grain yield increased with greater plant biomass and faster crop growth. - Longer flowering and maturity were associated with lower grain yield. ABSTRACT Rice (Oryza sativa L.) is a strategic crop in Southeast Asia, where seasonal variation strongly influences its growth, physiology, and yield. This study evaluated the agronomic performance and yield components of three rice genotypes representing local (Pandan Wangi), improved (Inpari 32), and hybrid (Intani 602) types across three planting periods within the wet season (early, mid, and late rainy season) in tropical lowland fields of East Java, Indonesia, during 2023 – 2024. The experiment was arranged in a randomized complete block design within each planting period, and data were analyzed using combined ANOVA, correlation analysis, and genotype by trait (GT) biplot. Results indicated that genotype and planting period significantly affected most agronomic and yield traits, with significant genotype × planting period interactions observed for several key variables. Grain yield was positively associated with biomass accumulation and growth efficiency (DW and CGR) and supported by canopy development (LAI), whereas phenological duration (DTF and DTM) showed negative associations with yield under the tested wet season conditions. GT biplot analysis explained 77.95% of the total variation (PC1 = 64.48%, PC2 = 13.47%) and indicated that hybrid combinations in the late rainy season planting period were closely associated with major yield and biomass traits, while Inpari 32 in the early rainy season planting period was more closely associated with radiation use indices (EPA and RUE) and tiller related traits. Pandan Wangi combinations were positioned nearer to phenological and v…
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creator: Muhammad Muharram
creator: Agus Suryanto
creator: Sudiarso
creator: Anna Satyana Karyawati
rights: Copyright (c) 2026 Muhammad Muharram, Agus Suryanto, Sudiarso, Anna Satyana Karyawati http://creativecommons.org/licenses/by-nc-nd/4.0
date: 2026-09-01
publication date: 2026-09-01
starting page: 303
ending page: 312
doi: 10.11598/btb.2026.33.3.2765

NATURE-BASED SOLUTIONS FOR RESILIENT RICE AGRO-ECOSYSTEMS
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description: ARTICLE HIGHLIGHTS - Nature-based tools restore biodiversity and self-regulating pest control. - Flowering strips and border crops boost beneficial predator populations. - Neem and custard apple biopesticides disrupt pests without toxicity. - Soil biochar and silicon build strong physical barriers in rice plants. - Integrated duck and fish farming suppresses pests while cutting chemical use. ABSTRACT The Green Revolution expanded global rice production but introduced systemic environmental vulnerabilities by eroding biodiversity and natural pest regulation. This critical review examines the strategic transition from pesticide-intensive cultivation to nature-based solutions (NbS) that prioritize ecological resilience. We synthesize diverse strategies, including ecological engineering, microbial tools, and integrated rice-animal farming, to demonstrate their collective efficacy in maintaining stable yields. Environmental manipulation through flowering strips supports a robust natural enemy complex, including the mirid bug (Cyrtorhinus lividipennis (Reuter)) and the egg parasitoid wasp (Trichogramma japonicum Ashmead). Furthermore, we evaluate the pivotal role of botanical insecticides from the neem tree (Azadirachta indica A. Juss.) and the sugar apple (Annona squamosa L)., alongside soil amendments like biochar, to induce systemic host resistance via silicon-mediated defenses. Crucially, we highlight how microbial biopesticides, including the entomopathogens Bt (Bacillus thuringiensis Berliner), green muscardine fungus (Metarhizium anisopliae (Metschn.) Sorokīn), and specialized granuloviruses, work in synergy with animal co-cultures involving the domestic duck (Anas platyrhynchos domesticus L.) and the common carp (Cyprinus carpio L.). Together, these synchronized interventions establish multi-layered barriers against major pests like the brown planthopper (Nilaparvata lugens (Stål)) and the yellow stemborer (Scirpophaga incertulas (Walker)). By aligning cultural, b…
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creator: Ravindra Chandra Joshi
creator: S. Shrivastava
creator: Annamalai Sivapragasam
creator: Anand Prakash
rights: Copyright (c) 2026 Dr. Ravindra C. Joshi, Dr. S. K. Shrivastava, Dr. Annamalai Sivapragasam, Dr. Anand Prakash http://creativecommons.org/licenses/by-nc-nd/4.0
date: 2026-07-17
publication date: 2026-07-17
starting page: 327
ending page: 358
doi: 10.11598/btb.2026.33.3.3052

WILD IN THE CITY: UNAUTHORIZED POSSESSION OF WILD FAUNA IN RESIDENCES OF VALENCIA CITY, BUKIDNON, PHILIPPINES
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description: ARTICLE HIGHLIGHTS - Covert surveys revealed undocumented wildlife possession within Valencia City residences. - Fifteen species were identified, including three threatened endemic species. - Philippine long-tailed macaque was documented in all study sites. - Human-wildlife interactions may have fueled locals to keep wild animals. - Findings advocate amnesty programs to bolster biodiversity conservation in the urban areas. ABSTRACT The illegal wildlife trade is a globally recognized industry, and the unauthorized possession of wildlife by locals is a significantly overlooked facet of this field. While significant seizures of poached animals were recorded in the country, many remain undocumented and undetected, specifically in small-scale collections or privately owned wildlife. This study assessed the ownership of wild fauna in five locations in Valencia City, Bukidnon, Philippines. A covert research approach was used to elicit information from the owners, which was done randomly within the months of March to April 2025. A total of 26 owners were interviewed, the majority are male (19; 73%), aged 40 – 45 years old (12; 46%), low-income farmers (17; 65%), and with primary education (16; 62%). The results revealed 63 individuals from 15 species: 8 species of birds, 4 species of mammals, and 3 species of reptiles. Of which, 12 (80%) species are classified as least concern, two (13%) are endangered, and one is undetermined. Birds are the most frequently recorded group (24 individuals), mainly intended for consumption, while Macaca fascicularis philippensis exist across all five barangays because of their behavioral flexibility and presumed human compatibility. Owners’ responses indicate that they keep such animals mainly for recreation or hobby (15; 25%), which reflects the affinity of locals to keep wild animals in the city may have been associated with human-wildlife interactions and humans’ familiarity with native and endemic animals. Therefore, the study recommends …
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creator: Khean Harvey Acuevas
creator: Adrian Aturo
creator: Dave Buenavista
rights: Copyright (c) 2026 Khean Harvey Acuevas, Adrian Aturo, Dave Buenavista http://creativecommons.org/licenses/by-nc-nd/4.0
date: 2026-06-19
publication date: 2026-06-19
starting page: 313
ending page: 326
doi: 10.11598/btb.2026.33.3.2571

OPTIMIZING eDNA METABARCODING IN INDONESIAN FRESHWATERS: A SCOPING REVIEW OF BEST PRACTICES
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description: ARTICLE HIGLIGHTS - Environmental DNA studies in Indonesian freshwaters remain uneven. - Multi-step filtering improves species detection and reduces false findings in tropical condition. - Taxa-specific primers detect native species better than broad-range primers. - Local databases and manual plausibility checks reduce wrong species matches. ABSTRACT Indonesian freshwater ecosystems harbor immense biodiversity yet remain understudied due to logistical constraints of conventional methods. Environmental DNA (eDNA) metabarcoding offers a noninvasive, high-resolution alternative for biodiversity monitoring, but its uptake in Indonesia is still nascent and methodologically heterogeneous. We reviewed 106 peer-reviewed studies (of which 28 studies were eligible) published between 2015 and 2025 across Google Scholar, PubMed, DOAJ, and GARUDA, charting sampling designs, molecular workflows, and bioinformatics pipelines. Studies were heavily skewed toward Java and West Sumatra (86%) and overwhelmingly employed filtration-based sampling. Broad-range COI and 12S markers dominated (39% and 36% of studies, respectively), whereas fish-specific MiFish-U primers, demonstrating superior sensitivity and specificity, were only used in 14% of cases. Correspondingly, non-specific primer in shotgun metagenomics proven imprudent. Studies using 0.22 µm filters collected a lot of species as much as non-target amplification, while 0.45 µm filters performed inconsistently. Bioinformatics approaches (QIIME2, DADA2, mBRAVE) differed widely but showed no clear impact on detection outcomes. Key limitations included geographic and taxonomic biases, poor reference library coverage, and a lack of expert-driven plausibility checks. We recommend a standardized workflow combining coarse pre-filtration, taxa-specific primers (e.g., MiFish for fish), and manual validation against expanded local databases to strengthen eDNA-based biodiversity assessments in Indonesia.
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creator: I Dewa Agung Panji Dwipayana
creator: Ni Luh Putu Kayika Febryanti
creator: Yan Ramona
rights: Copyright (c) 2026 I Dewa Agung Panji Dwipayana, Ni Luh Putu Kayika Febryanti, Yan Ramona http://creativecommons.org/licenses/by-nc-nd/4.0
date: 2026-07-28
publication date: 2026-07-28
starting page: 403
ending page: 412
doi: 10.11598/btb.2026.33.3.2639

CARBON STOCK ESTIMATION IN FABACEAE TREE STANDS WITHIN THE URBAN FORESTSOF BANDA ACEH
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description: ARTICLE HIGHLIGHTS - Identified four Fabaceae tree species across three urban forests in Banda Aceh. - Total estimated potential carbon stock in Fabaceae stands reached 292.08 tonnes/ha. - Trembesi Urban Forest contributed the highest carbon stock at 124.14 tonnes/ha. - Samanea saman is the dominant carbon contributor, storing 248.44 tonnes/ha. - Stem diameter serves as a highly reliable predictor for urban tree carbon stocks. ABSTRACT Urban forests play a crucial role in absorbing carbon emissions through the biomass carbon stocks stored in vegetation. The Fabaceae family is a potential group of tree for carbon storage, but its contribution to the Banda Aceh urban forest area remains understudied. This study aims to identify tree species from the Fabaceae family and estimate the potential carbon stocks stored in stands of this family in the Banda Aceh urban forest. The study was conducted in three urban forest locations: Putroe Phang (PP), Trembesi (TR), and BNI Urban Forests (BNI). Carbon stocks were estimated using a non-destructive method with an allometric equation approach. The results showed that there were four tree species from the Fabaceae family, namely Samanea saman, Pterocarpus indicus, Tamarindus indica, and Delonix regia, with a total of 197 individuals recorded. The total potential carbon stocks stored in Fabaceae stands in the three urban forest areas reached 292.08 tons/ha, with the largest contribution coming from the Trembesi (TR) Urban Forest at 124.14 tons/ha. The tree species that provides the largest contribution to carbon biomass is Samanea saman with an estimate of 248.44 tons/ha.
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creator: Wira Dharma
creator: Yekki Yasmin
creator: Nada Ariqah
creator: Essy Harnelly
creator: Alia Rizki
creator: Amirunnas
creator: Anita Rauzana
creator: Rahmad Zakaria
rights: Copyright (c) 2026 Wira Dharma, Yekki Yasmin, Nada Ariqah, Essy Harnelly, Alia Rizki, Amirunnas, Anita Rauzana, Rahmad Zakaria http://creativecommons.org/licenses/by-nc-nd/4.0
date: 2026-07-20
publication date: 2026-07-20
starting page: 359
ending page: 368
doi: 10.11598/btb.2026.33.3.2687

YEAST EXTRACT ELICITATION ENHANCES METABOLITE PRODUCTION IN Alocasia cuprea CALLUS
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description: ARTICLE HIGLIGHTS Yeast extract boosts metabolites Enhances bioactive yield Best at moderate dose (150 mg/L) ABSTRACT Alocasia cuprea is a tropical ornamental plant species belonging to the family Araceae, widely recognized for its distinctive and aesthetically appealing foliage. Additionally, it represents a promising source of bioactive metabolites. This study aimed to evaluate the potential of yeast extract elicitation for enhancing metabolite production in A. cuprea callus culture established through in vitro propagation. The methods used in this study included callus induction, elicitation treatments, and phytochemical analysis of A. cuprea callus. The experiment was conducted using a completely randomized design (CRD) consisting of five yeast extract concentrations (0, 50, 100, 150, and 200 mg/L) with six replications. The results revealed that elicitation treatment did not affect callus biomass. Morphologically, the callus exhibited a friable texture and changed in color from off-white to yellowish-brown following elicitation. However, elicitation increased both the yield and diversity of metabolites in A. cuprea callus cultures. The optimal yeast extract concentration for enhancing the accumulation of major metabolites in A. cuprea was 150 mg/L. At this concentration, the levels of n-hexadecanoic acid, 9,12-octadecadienoic acid (Z,Z)-, and squalene increased by 6.67%, 82.49%, and 291.60%, respectively, compared with control. Nevertheless, the accumulation patterns of individual compounds varied across treatments, indicating that each metabolite exhibits a specific biosynthetic response to variations in yeast extract concentration.
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creator: Zozy Aneloi Noli
creator: Suwirmen
creator: Lily Ismaini
creator: Meza Rani Maudya
rights: Copyright (c) 2026 Zozy Aneloi Noli, Suwirmen, Lily Ismaini, Meza Rani Maudya http://creativecommons.org/licenses/by-nc-nd/4.0
date: 2026-07-21
publication date: 2026-07-21
starting page: 369
ending page: 377
doi: 10.11598/btb.2026.33.3.2649

ETHNOBOTANICAL STUDY OF MANGROVES FROM COASTAL COMMUNITIES OF BUNAKEN NATIONAL PARK, INDONESIA
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description: ARTICLE HIGLIGHTS Mangrove species like Acanthus ilicifolius and Avicennia marina aid food and health. Correlation and PCA reveal food, medicine, and firewood as key use dimensions. Location trends: Sondaken for firewood, Baboi for medicine, Mantehage for knowledge. This study supports mangrove ethnobotany and promotes community bioconservation. ABSTRACT Mangrove ecosystems play a vital ecological and socio-economic role along coastal regions. However, their sustainable utilization faces significant challenges due to habitat degradation and modernization. This study aimed to characterize the ethnobotanical utilization of mangroves by coastal communities within Bunaken National Marine Park (BNMP) and its surrounding small islands, while exploring their potential for food and medicinal bioprospecting. Data were collected through questionnaire surveys, in-depth interviews with informants, and field observations within coastal communities in four locations such as Bahoi, Likupang Barat District, North Minahasa, Sondaken, Tatapaan District, South, Alung Banua, Bunaken Archipelago District, Manado, and Mantehage, Wori District, North Minahasa Regency. The data were subsequently analyzed descriptively, followed by Principal Component Analysis (PCA) and heatmap visualization. The findings revealed that specific species such as Acanthus ilicifolius and Avicennia marina occupy central roles as sources of food and traditional medicine, emphasizing the close integration between subsistence needs and community health. Correlation analysis and PCA indicated that the categories of food, medicine, and firewood represent the dominant dimensions of utilization, while cultural functions and species knowledge serve as secondary differentiators. Location-based variations underscored the heterogeneity of utilization practices, with Sondaken being more prominent in firewood use, Bahoi in ethnomedicine, and Mantehage in biodiversity knowledge. These findings highlight the necessity of mang…
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creator: Nova Laurin Isye Mourein Ogi
creator: Fernando A Watung
creator: Mercy M Rampengan
creator: Meisa Tabita Rogahang
creator: Verawati Ida Yani Roring
creator: Mokosuli Yermia Semuel
rights: Copyright (c) 2026 Nova Laurin Isye Mourein Ogi, Fernando A Watung, Mercy M Rampengan, Meisa Tabita Rogahang, Verawati Ida Yani Roring, Mokosuli Yermia Semuel http://creativecommons.org/licenses/by-nc-nd/4.0
date: 2026-07-23
publication date: 2026-07-23
starting page: 389
ending page: 402
doi: 10.11598/btb.2026.33.3.2748

DIVERSITY OF SMALL NON-VOLANT MAMMALS IN THE CENTER FOR ECOLOGICAL DEVELOPMENT AND RECREATION (CEDAR), BUKIDNON, PHILIPPINES
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description: ARTICLE HIGLIGHTS - Six murid rodent species, including one endemic and five non-native species, were recorded in CEDAR, Bukidnon. - Semi-natural habitats were dominated by endemic (Rattus cf. everetti), while human-modified habitats were dominated by non-native rodents. - Human-modified habitats had higher rodent diversity than semi-natural habitats. - Rattus tanezumi and Rattus cf. exulans adapted to both habitat types. - Semi-natural habitats are vital for conserving native rodent diversity. ABSTRACT This study assessed the diversity and species composition of murid rodents in two habitat types: semi-natural and human-modified within the Center for Ecological Development and Recreation (CEDAR) in Impasug-ong, Bukidnon, Philippines. A total of 640 trap nights were conducted using live traps across both habitats, yielding six (6) rodent species: one (1) native (Rattus cf everetti) and five non-native species (Rattus cf exulans, Rattus cf norvegicus, Rattus tanezumi, Mus musculus, and an unidentermined Rattus species). Station 1 (semi-natural habitat) was dominated by the native species, while Station 2 (human-modified habitat) was dominated by non-native species. Diversity analysis using the Shannon-Wiener Index showed higher diversity in the human-modified habitat (H’ = 1.280) compared to the semi-natural habitat (H’ = 1.004), supporting the hypothesis that habitat modification influences rodent diversity. The exclusive presence of R. cf. everetti in the semi-natural habitat emphasizes the sensitivity of native species to disturbance while R. tanezumi and R. cf. exulans were found in both habitats, indicating their adaptability to varied environments. These findings support the hypothesis that habitat modification influences rodent diversity and human-modified habitat are more species rich dominated by non-native species. The study highlights the importance of conserving semi-natural habitats and highlights the utility of rodents as bioindicators for assessing eco…
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creator: Jasmin Rizalda
creator: Dave Buenavista
rights: Copyright (c) 2026 Jasmin Rizalda, Dave Buenavista http://creativecommons.org/licenses/by-nc-nd/4.0
date: 2026-07-28
publication date: 2026-07-28
starting page: 413
ending page: 423
doi: 10.11598/btb.2026.33.3.2567

HEPATOPROTECTIVE EFFECT OF Physalis angulata L. LEAF ETHANOL EXTRACT AGAINST BUSULFAN-INDUCED LIVER DAMAGE IN FEMALE RATS
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description: ARTICLE HIGLIGHTS Busulfan can damage the liver by triggering oxidative stress Physalis angulata leaves show natural liver protective effects Extract reduced oxidative damage in liver tissue Liver structure improved after extract treatment Early treatment provided stronger liver protection than delayed use ABSTRACT Liver disease is the leading cause of death worldwide, with drug-induced hepatotoxicity representing a major contributing factor. Busulfan, an anticancer drug used in chronic myeloid leukemia, is cytotoxic to proliferating cells and can induce liver injury. Physalis angulata L. (ground cherry) contains bioactive compounds with potential hepatoprotective properties. This study aimed to evaluate the protective effects of ethanol extract of Phyasalis angulata L. (EEPA) leaves on busulfan-induced liver damage in female Sprague–Dawley rats. Twenty-five rats were randomly divided into five groups: P1 (normal control), P2 (busulfan 40 mg/kg BW, single dose), P3 (extract 300 mg/kg BW, days 1–28), P4 (busulfan + extract, days 1–28), and P5 (busulfan + extract, days 14–28). Parameters assessed were alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the level of malondialdehyde (MDA) in liver, and liver histology. Data were analyzed using one-way analysis of variance (ANOVA) followed by a post hoc test. The results showed that Physalis angulata L. extract did not cause significant differences in the serum ALT and AST levels but significantly reduced the MDA concentrations and improved the liver histological architecture. Busulfan administration significantly decreased hepatocyte count and increased Kupffer and inflammatory cells, fatty degeneration, and necrosis. Early administration of the extract (P4) demonstrated stronger hepatoprotective effects compared with delayed administration (P5). These findings suggest that EEPA leaves exerts hepatoprotective effects against busulfan-induced liver injury in rats, likely through antioxidant activity, as …
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creator: Elsi Rahmadhani
creator: Aryani Sismin Satyaningtijas
creator: Damiana Rita Ekastuti
creator: Ronald Tarigan
creator: I Ketut Mudite Adnyane
creator: Kusdiantoro Mohamad
creator: Wahono Esthi Prasetyaningtyas
creator: Yanti Sulistyana
rights: Copyright (c) 2026 Elsi Rahmadhani, Aryani Sismin Satyaningtijas, Damiana Rita Ekastuti, Ronald Tarigan, I Ketut Mudite Adnyane, Kusdiantoro Mohamad, Wahono Esthi Prasetyaningtyas, Yanti Sulistyana http://creativecommons.org/licenses/by-nc-nd/4.0
date: 2026-07-23
publication date: 2026-07-23
starting page: 378
ending page: 388
doi: 10.11598/btb.2026.33.3.2588

RELATIONSHIP BETWEEN FIBER MORPHOLOGY AND PARALLEL-TO-GRAIN MECHANICAL STRENGTH IN FOUR BAMBOO SPECIES GROWNIN THE PHILIPPINES
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description: ARTICLE HIGLIGHTS The study evaluated the fiber morphology and mechanical properties of four bamboo species, revealing the species-specific relationships of fiber traits and mechanical performance. Cell wall thickness and lumen width influenced bamboo’s mechanical strength. Solid bamboo exhibits the highest compression despite weak fiber links; Yellow bamboo displays the highest shear with the longest fibers; Thailand bamboo demonstrates the strongest fiber traits-strength correlations. ABSTRACT The compression and shear strength parallel to the grain of four bamboo species namely Yellow bamboo (Bambusa vulgaris Schrad. ex J.C.Wendl.), Iron bamboo (Guadua angustifolia Kunt.), Solid bamboo [Dendrocalamus strictus (Roxb.) Nees], and Thailand bamboo (Thyrsostachys siamensis Gamble) were evaluated in relation to their fiber morphology. Results revealed distinct species-specific patterns. Thailand bamboo exhibited the strongest positive correlations, particularly between fiber length, fiber diameter, cell wall thickness, and both strength properties. Yellow bamboo recorded the longest fibers and the highest shear strength, with strong associations to cell wall thickness. In contrast, Solid bamboo showed the highest compression strength but weak correlations with fiber characteristics, while Iron bamboo demonstrated moderate associations between fiber morphology and mechanical properties. Among all fiber characteristics, cell wall thickness consistently showed a strong positive correlation with strength across species, whereas lumen width was negatively correlated, indicating that denser, more compact fiber structures enhance load-bearing capacity. Principal Component Analysis further confirmed the dominant influence of cell wall thickness, with variable contributions from fiber length and diameter depending on species. These findings highlight the anatomical basis of bamboo’s mechanical performance and emphasize the need for species-specific approaches in its structural …
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creator: Oliver S. Marasigan
creator: Mario Angelo M. Mundn
creator: Shereyl A. Daguinod
creator: Emmanuel P. Domingo
creator: Jayric F. Villareal
rights: Copyright (c) 2026 Oliver S. Marasigan, Mario Angelo M. Mundn, Shereyl A. Daguinod, Emmanuel P. Domingo, Jayric F. Villareal http://creativecommons.org/licenses/by-nc-nd/4.0
date: 2026-07-29
publication date: 2026-07-29
starting page: 424
ending page: 437
doi: 10.11598/btb.2026.33.3.2595