TY - JOUR AU - Poerba, Yuyu Suryasari AU - Martanti, Diyah AU - Ahmad, Fajarudin PY - 2019/01/24 Y2 - 2024/03/29 TI - GENETIC VARIATION OF WILD Musa acuminata COLLA FROM INDONESIA JF - BIOTROPIA JA - BIOTROPIA VL - 26 IS - 2 SE - Research Paper DO - 10.11598/btb.2019.26.2.896 UR - https://journal.biotrop.org/index.php/biotropia/article/view/896 SP - 115-126 AB - <p>Indonesia is the center of origin and diversity of <em>Musa acuminata</em> Colla, one of the progenitors of cultivated bananas today<em>.</em> However, the genetic variation of wild <em>M. acuminata</em> has not been studied extensively, thus this study aimed to assess the genetic variation of the Indonesian wild <em>M. acuminata</em> based on 10 RAPD (Random Amplified Polymorphic DNA) and 10 ISSR (Inter Simple Sequence Repeats) markers. The genetic properties of 17 populations of wild <em>M. acuminata</em> were analyzed by Popgene 1.32 based on Nei’s unbiased measures of genetic identity and genetic distance. Of the 443 DNA bands produced, 425 (95.94%) were polymorphic. Cluster analysis of the combined data of RAPD and ISSR produced a dendrogram which separated the population of <em>M. acuminata</em> (A genome) from <em>M. balbisiana</em> (B genome), but not from <em>M. schizocarpa</em> (S genome).  Nei’s genetic distance of the 17 populations of wild <em>M. acuminata</em> ranged from 0.3676 to 0.1634.  The highest genetic distance was observed between <em>M. acuminata</em> var <em>rutilifes </em>(from East Java) and <em>M. acuminata</em> var <em>sumatrana </em>(from West Sumatra). The percentages of polymorphic loci among the 17 populations of <em>M. acuminata </em>ranged from 9.93% to 39.73%. Nei’s gene diversity (h) ranged from 0.041 to 0.1418. <em>M. acuminata</em> var <em>malaccensis</em> population was the most diverse among the researched 17 <em>M. acuminata</em>. The high level of genetic diversity of the wild <em>M. acuminata</em> from Indonesia emphasizes the need for conservation and preservation of the natural population and its use in the banana breeding program.</p> ER -