TY - JOUR AU - SUKENDAH, SUKENDAH AU - CEDO, MA L. O PY - 2011/11/17 Y2 - 2024/03/29 TI - IN VITRO CONSERVATION OF COCONUT (Cocos nucifera L.) EMBRYOS IN CULTURE MEDIA JF - BIOTROPIA JA - BIOTROPIA VL - 0 IS - 25 SE - Research Paper DO - 10.11598/btb.2005.0.25.210 UR - https://journal.biotrop.org/index.php/biotropia/article/view/210 SP - AB - <br />The possibility of delaying the germination of mature coconut embryos  in vitro for medium-term <br />conservation of coconut was investigated.  Embryos of coconut was stored in sugar free, full-strength and <br />half-strength MS media for 3 months with minimal loss in viability.  Germinability of embryos decreased <br />with prolonged storage.  At the end of 9-month storage period, embryos kept in full-strength media were <br />all non-germinable, while those stored in half-strength media still gave 15% germination.  Addition of <br />mannitol up to 0.3M did show any significant effect  on germination of embryos stored for 3 months.  <br />However, longer period of storage helps to a certain extent in the preservation of embryo viability.  The <br />presence of mannitol in the storage medium also induced morphological abnormalities in seedlings <br />derived subsequently from the stored embryos.  Mannitol at 0.4M was totally deleterious to the embryo.  <br />Significance of these findings on coconut genetic resources conservation are discussed. <br /> <br />Keywords:  In vitro conservation/Coconut embryo. ER -