TY - JOUR AU - SUHARNA, N AU - KIKUCHI, Y. AU - FUKATSU, T. PY - 2011/11/17 Y2 - 2024/03/29 TI - MOLECULAR PHYLOGENETIC ANALYSIS OF MONASCUS FUNGI BASED ON INTERNAL TRANSCRIBED SPACER REGION JF - BIOTROPIA JA - BIOTROPIA VL - 0 IS - 24 SE - Research Paper DO - 10.11598/btb.2005.0.24.173 UR - https://journal.biotrop.org/index.php/biotropia/article/view/173 SP - AB - A molecular analysis of internal transcribed spacer region has been carried out to reveal the relationship among <br />16 strains of Monascus spp. A primer set comprised primer ITS1 and ITS4 was used to amplify this region in which they <br />were cloned and scqucnccd. We also compared the sequence result with M. purpureus AF458473, M.ruber AF458470, M. <br />kaoliang AF451859, M. araneous AF458471 and M. pilosus AF451856 and one outgroup species Thermoascus crustaceus <br />U18353. <br />The result showed that 16 Monascus spp. were divided into two large clades while M. ruber AF458470 was <br />basically separated from all those Monascus. One of the two large clades included the seven M. purpureus strains, M. <br />purpureus AF458473, M. araneosus AF458471 and M. kaoliang AF451859. Another large cladc included the six <br />Monascus sp. strains which typically have whitish colonies, the three M. ruber strains and M.pilosus AF451856. <br />However, even outstanding morphological differences possessed by several white Monascus  and one whitish M. <br />purpureus  strain, all Monascus  strains were suggested to be very closely related with similarity >99% almost 100%. <br />Although this ITS analysis could not discriminate cultural and morphological differentiation of Monascus strains studied, <br />yet there is still little genetic variation within these strains. <br />Key words : Molecular genetics/Monascus spp./fungi ER -