@article{SUNARTI_MERYANDINI_SOFIYANTO_RICHANA_2024, title={SACCHARIFICATION OF CORNCOB USING CELLULOLYTIC BACTERIA FOR BIOETHANOL PRODUCTION}, volume={17}, url={https://journal.biotrop.org/index.php/biotropia/article/view/80}, DOI={10.11598/btb.2010.17.2.80}, abstractNote={<!-- [if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> </xml><![endif]--><!-- [if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> </xml><![endif]--><!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} p.ListParagraph, li.ListParagraph, div.ListParagraph {mso-style-name:"List Paragraph"; margin-top:0cm; margin-right:0cm; margin-bottom:0cm; margin-left:36.0pt; margin-bottom:.0001pt; mso-add-space:auto; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} p.ListParagraphCxSpFirst, li.ListParagraphCxSpFirst, div.ListParagraphCxSpFirst {mso-style-name:"List ParagraphCxSpFirst"; mso-style-type:export-only; margin-top:0cm; margin-right:0cm; margin-bottom:0cm; margin-left:36.0pt; margin-bottom:.0001pt; mso-add-space:auto; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} p.ListParagraphCxSpMiddle, li.ListParagraphCxSpMiddle, div.ListParagraphCxSpMiddle {mso-style-name:"List ParagraphCxSpMiddle"; mso-style-type:export-only; margin-top:0cm; margin-right:0cm; margin-bottom:0cm; margin-left:36.0pt; margin-bottom:.0001pt; mso-add-space:auto; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} p.ListParagraphCxSpLast, li.ListParagraphCxSpLast, div.ListParagraphCxSpLast {mso-style-name:"List ParagraphCxSpLast"; mso-style-type:export-only; margin-top:0cm; margin-right:0cm; margin-bottom:0cm; margin-left:36.0pt; margin-bottom:.0001pt; mso-add-space:auto; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --><!-- [if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} </style> <![endif]--> <p class="ListParagraph" style="margin: 0cm 0cm 10pt; text-align: justify; text-indent: 21.25pt; line-height: 150%;">The use of cellulose degrading enzyme (cellulases) for hydrolysis of lignocellulosic material is a part of bioethanol production process.  In this experiment,  delignified corncob,  its cellulose fraction and alpha cellulose were used as s<span style="background: yellow none repeat scroll 0% 0%; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;">ubstrates</span> to produce <span lang="IN">fermentable</span> sugar by using three local isolates of celluloytic bacteria <span style="background: yellow none repeat scroll 0% 0%; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;">(</span>C5-1, C4-4, C11-1 and Cmix ; mixed cultures of three isolates), and <em>Saccharomyces cereviseae </em>to produce ethanol. The results <span style="background: yellow none repeat scroll 0% 0%; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;">showed</span> that all isolates of cellulolytic bacteria can grow on cellulose fraction better than on  delignified corncob, and alpha cellulose.  <span style="background: yellow none repeat scroll 0% 0%; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;">The</span> highest hydrolytic activity produced from cellulose fraction <span style="background: yellow none repeat scroll 0% 0%; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;">was by isolate C4-4, which</span> liberated 3.50 g/l of total sugar.  <span style="background: yellow none repeat scroll 0% 0%; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;">Ethanol can be produced by mixed culture of bacteria and yeast, but because of competitive growth, the fermentation only produced 0.39-0.47 g/l of ethanol</span>.</p> <p class="ListParagraph" style="margin: 0cm 0cm 10pt; text-align: justify; text-indent: 21.25pt; line-height: 150%;">Keywords:  Celluloses, delignified corncob</p>}, number={2}, journal={BIOTROPIA}, author={SUNARTI, TITI CANDRA and MERYANDINI, ANJA and SOFIYANTO, M. EDY and RICHANA, NUR}, year={2024}, month={Jan.} }