@article{YUHANA_HORATH_HANSELMANN_2011, title={BACTERIAL COMMUNITY SHIFTS OF A HIGH MOUNTAIN LAKE IN RESPONSE TO VARIABLE SIMULATED CONDITIONS: AVAILABILITY OF NUTRIENTS, LIGHT AND OXYGEN}, volume={13}, url={https://journal.biotrop.org/index.php/biotropia/article/view/219}, DOI={10.11598/btb.2006.13.2.219}, abstractNote={We studied bacterial population composition shifts by exposing natural water samples to variable simulated environmental conditions. <br />The samples were taken from Lake Jori XIII (2640 m a.s.l), an oligo-to mesotrophic cold freshwater lake, located in the eastern Swiss <br />Alps. The Jori lakes are characterized as remote, unpolluted high mountain lakes with a long period of ice cover and typically low <br />nutrient concentrations. Culture independent techniques (PCR-based analyses) were used for detection and molecular <br />characterization of a large number of bacteria most of which are still uncultivable. Bacterial community shifts over three ecological <br />conditions (nutrients, light and oxygen availability) were detected by using Temporal Temperature gradient Gel Electrophoresis (TTGE) <br />of a PCR-amplified part of the 16S rRNA gene. The bacterial populations responded differently to the variable conditions, as <br />revealed by TTGE pattern shifts during the experiment. <br />Key words: Temporal Temperature gradient Gel Electrophoresis (TTGE), ARB, small subunit ribosomal RNA gene (SSU rRNA gene), <br />alpine freshwater lake JOri XIII, PCR}, number={2}, journal={BIOTROPIA}, author={YUHANA, MUNTI and HORATH, THOMAS and HANSELMANN, KURT}, year={2011}, month={Aug.} }