@article{RATNADEWI_2011, title={LOCALIZATION OF GFDD4-1 EXPRESSED PROTEIN IN Physcomitrella patens CELLS}, volume={13}, url={https://journal.biotrop.org/index.php/biotropia/article/view/214}, DOI={10.11598/btb.2006.13.1.214}, abstractNote={The expression of a new dehydration-related gene of Physcomitrella patens, GFDD4-I, was traced for its localization <br />in the plant cells. This revelation is useful to predict the possible roles of the protein in plant tolerance to environmental <br />stress. This gene was fused to  gfp  marker gene and transfected into the plant protoplasts. Under a confocal laser <br />microscope, it was detected that the GFDD4-1 protein associated with the OFF started to generate at the cell periphery <br />and developed more intensively inwards to cytoplasm, forming vesicles and cystemal structures or network. The protein <br />might be membrane protein which may involve directly in membrane maintenance or cellular protection against stress <br />conditions. <br />Key words : Protoplast transformation, protein localization, dehydration-related gene, GFP, Physcomitrella patens}, number={1}, journal={BIOTROPIA}, author={RATNADEWI, DIAH}, year={2011}, month={Aug.} }