@article{WIDANARNI_SUWANTO_SUKENDA_LAY_2011, title={POTENCY OF VIBRIO ISOLATES FOR BIOCONTROL OF VIBRIOSIS IN TIGER SHRIMP (PENAEUS MONODON) LARVAE}, url={https://journal.biotrop.org/index.php/biotropia/article/view/180}, DOI={10.11598/btb.2003.0.20.180}, abstractNote={<br />This study was carried out to obtain Vibrio isolates able to function as biocontrol of vibriosis in shrimp hatchery. <br />Thirty one Vibrio isolates were isolated from tiger shrimp larvae and hatchery environments, i.e. Labuan, Pangandaran, and <br />Lampung, Indonesia. Pathogenic  V. harveyi MR5339 was obtained from Maros, South-Sulawesi and was made as a <br />rifampicin resistant mutant (RFR<br />) to screen for those 31 Vibrio isolates in in vitro assays and to allow us to monitor their <br />presence in shrimp larvae and larval rearing water. Almost all Vibrio  isolates could inhibit the growth of pathogenic V. <br />harveyi MR5339 RFR<br />. SKT-b isolate from Skeletonema was the most effective to inhibit the growth of V. harveyi MR5339 Rf* <br />and significantly reduced larval mortality in pathogen challenge assays. These prospective biocontrol bacteria, at concentration <br />of 10" CFU/ml, did not show pathogenicity to shrimp larvae. SKT-b was Gram negative, short rod-shape, exhibited yellow <br />colonies on TCBS and swarming on SWC-agar media, motile, utilized glucose and sucrose but not lactose: produced extra-<br />cellular protease and amylase, but did not produce chitmase. Partial sequencing of 16S-rRNA gene SKT-b showed <br />SKT-b similarity to Vibrio alginofyticus. <br />Keywords: shrimp larvae / biocontrol bacteria / vibriosis.}, number={20}, journal={BIOTROPIA}, author={WIDANARNI, WIDANARNI and SUWANTO, A. and SUKENDA, SUKENDA and LAY, B. W.}, year={2011}, month={Nov.} }