REGULATION OF ADIPOGENESIS AND KEY ADIPOGENIC GENE EXPRESSION BY MANGOSTEEN PERICARP EXTRACT AND XANTHONES IN 3T3-L1 CELLS

Authors

  • Wahyu Widowati Medical Research Center, Faculty of Medicine, Maranatha Christian University, Jl. Prof. Drg. Surya Sumantri No. 65 Bandung 40164, West Java, Indonesia
  • Lusiana Darsono Faculty of Medicine, Maranatha Christian University, Jl. Prof. drg. Suria Sumantri no 65, Bandung 40164, Indonesia
  • Jo Suherman Faculty of Medicine, Maranatha Christian University, Jl. Prof. drg. Suria Sumantri no 65, Bandung 40164, Indonesia
  • Ervi Afifah Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk 2 No. 9, Bandung 40163, Indonesia
  • Rizal Rizal Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk 2 No. 9, Bandung 40163, Indonesia
  • Yukko Arinta Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk 2 No. 9, Bandung 40163, Indonesia
  • Tjandrawati Mozef Research Center of Chemistry, Indonesian Institute of Sciences (LIPI) Bandung, Jl. Cisitu, Sangkuriang Bandung 40135, Indonesia
  • Tri Suciati School of Pharmacy Bandung Insitute of Technology, Bandung, West Java, Indonesia

DOI:

https://doi.org/10.11598/btb.2020.27.1.932

Keywords:

adipogenesis, atherosclerosis, gene expression, mangosteen, obesity

Abstract

Obesity is one of the risk factors for atherosclerosis and its fat occurrence and development are associated with fat accumulation and adipocyte differentiation.  Thus, the suppression of adipocyte differentiation can be a potential anti-obesity approach to this health concern. This study examined the effect of mangosteen pericarp extract (MPE) and xanthone (α-Mangostin (AM) and γ-Mangostin (GM)) on the expression of PPARγ, C/EBPα, SCD1, LPL, aP2, adipoQ, and FAS in 3T3-L1 cells. Concentrations of MPE and xanthones used were based on the cytotoxic assay on 3T3-L1 cells. Three different MPE concentrations (0, 25, and 50 µg/ml) and three different AM concentrations (0, 25 and 50 µM) and GM (0, 50, and 75 µM) were used in the experiment. The expressions of PPARγ, C/EBPα, SCD1, LPL, aP2, adipoQ, and FAS genes were measured using real-time quantitative PCR. The expression of the genes was down-regulated in the group of cells treated with 50 µg/ml of MPE and 50 µM of GM. However, the 25 µM and 50 µM of AM did not suppress PPARγ and SCD-1 expression. The 50 µM of AM also failed to reduce aP2 gene expression. Finally, MPE and GM showed potential anti-adipogenesis and anti-obesity effects by suppressing the expression of PPARγ, C/EBPα, SCD1, LPL, aP2, adipoQ and FAS genes in 3T3-L1 cells.

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Published

2019-08-28

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Section

Research Paper