REGULATION OF ADIPOGENESIS AND KEY ADIPOGENIC GENE EXPRESSION BY MANGOSTEEN PERICARP EXTRACT AND XANTHONES IN 3T3-L1 CELLS
Keywords:adipogenesis, atherosclerosis, gene expression, mangosteen, obesity
AbstractObesity is one of the risk factors for atherosclerosis and its fat occurrence and development are associated with fat accumulation and adipocyte differentiation.Â Thus, the suppression of adipocyte differentiation can be a potential anti-obesity approach to this health concern. This study examined the effect of mangosteen pericarp extract (MPE) and xanthone (Î±-Mangostin (AM) and Î³-Mangostin (GM)) on the expression of PPARÎ³, C/EBPÎ±, SCD1, LPL, aP2, adipoQ, and FAS in 3T3-L1 cells. Concentrations of MPE and xanthones used were based on the cytotoxic assay on 3T3-L1 cells. Three different MPE concentrations (0, 25, and 50 Âµg/ml) and three different AM concentrations (0, 25 and 50 ÂµM) and GM (0, 50, and 75 ÂµM) were used in the experiment. The expressions of PPARÎ³, C/EBPÎ±, SCD1, LPL, aP2, adipoQ, and FAS genes were measured using real-time quantitative PCR. The expression of the genes was down-regulated in the group of cells treated with 50 Âµg/ml of MPE and 50 ÂµM of GM. However, the 25 ÂµM and 50 ÂµM of AM did not suppress PPARÎ³ and SCD-1 expression. The 50 ÂµM of AM also failed to reduce aP2 gene expression. Finally, MPE and GM showed potential anti-adipogenesis and anti-obesity effects by suppressing the expression of PPARÎ³, C/EBPÎ±, SCD1, LPL, aP2, adipoQ and FAS genes in 3T3-L1 cells.
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