SCREENING OF THE WHOLE EGG WHITE PROTEINS IN VARIABLE TYPES OF BIRDS

Mohamed Baqur S. Al-Shuhaib, Hayder O. Hashim, Mufeed J. Ewadh

Abstract


The comparative heterogeneity of the detailed, in-parallel protein composition data analysis for wide varieties of birds’ egg white samples has not yet been fully defined. The main object of this research is to evaluate the extent of variability among more than 40 types of birds’ egg white. To improve the perception of these biological fluids, the main phenotypes variations of egg white were evaluated using the discontinuous denaturing polyacrylamide gel electrophoresis (SDS-PAGE), Gradient SDS-PAGE, Native-PAGE, cellulose acetate electrophoresis, and reverse phase high-performance liquid chromatography (RP-HPLC). Though the latest techniques didn’t show significant variability in terms of hydrophobicity, several electrophoretic differences of egg-white proteins were observed. As well, several unknown proteins in many egg white samples of different bird species were identified through electrophoretic experiments. So, it might be possible, as it shown in many cases of egg white samples, to provide a characterized assessment among birds only by using the available gel electrophoresis techniques. Also, this study provides a rapid snapshot for the initial identification of several unknown egg white protein components. According to our knowledge, this study constitutes the first large-scale comparative proteomics investigation performed among these largely variable types of egg white samples.


Keywords


Egg white, Gradient-PAGE, HPLC, Native-PAGE, SDS-PAGE

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DOI: http://dx.doi.org/10.11598/btb.2019.26.2.812

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