VIABILITY AND PLASMA MEMBRANE INTEGRITY OF THE SPOTTED BUFFALO EPIDIDYMAL SPERMATOZOA AFTER THAWING WITH THE ADDITION OF DEXTROSE INTO THE EXTENDER

YULNAWATI YULNAWATI, H. MAHESHWARI , HERDIS , M. RIZAL

Abstract


The objective of this study was to obtain the viability and plasma membrane integrity of the spotted buffalo epididymal sperm after addition of dextrose into AndromedÒ extender.  Spermatozoa that have been collected from cauda epididymis were diluted with AndromedÒ extender as control (K) and AndromedÒ + 0.2% dextrose (P1) and AndromedÒ + 0.4% dextrose (P2) as treatments.  The results showed that the quality of epididymal spermatozoa decreased during cryopreservation process.  The percentage of motility after thawing in P1 (46%) and P2 (46.67%) were significantly higher (P<0.05) compared to K (41%) as well as the percentage of live sperm in P1 (58.8%) and P2 (60%) compared to K (52.2%). The percentage of membrane integrity in P1, P2 and K were 67.4; 66.8 and 68 %, respectively.  In conclusion, the addition of 0.2 and 0.4% of dextrose into AndromedÒ acted as an extra cellular cryoprotectant and could maintain the viability and membrane integrity of the spotted buffalo epididymal spermatozoa after thawing.

 

Key words: epididymal sperm, cryopreservation, dextrose, spotted buffalo


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DOI: http://dx.doi.org/10.11598/btb.2009.16.1.63

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